Complement and Inflammation
Original Papers
Further Evidence for Dilution-Dependent Dissociation of Clq in Human SerumHosoi S. · Borsos T.Laboratory of Immunobiology, National Cancer Institute, National Institutes of Health, FCRF, Frederick, Md., USA
Keyword: ComplementClq dissociation |
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Article / Publication Details
Published online: August 11, 2017
Issue release date: 1989
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 1012-8204 (Print)
eISSN: 2504-2416 (Online)
For additional information: https://www.karger.com/CID
Abstract
Dissociation of the Clq subcomponent in native Cl upon dilution was reexamined by using ultracentrifugation in a sucrose gradient and high performance liquid chromatography system with a size exclusion column for separating the dissociated Clq fractions. The antigenic content of Clq in each fraction was detected by ELISA and Western blotting; binding to erythrocyte antibody was also determined. The results confirmed a previous claim that Clq in native Cl dissociated as a function of dilution: up to 14.5% of Clq antigen was in the low molecular weight form (approximate S value: 4-5). Commercial preparations of purified Clq also contained Clq antigen in the low molecular weight form.
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Article / Publication Details
Published online: August 11, 2017
Issue release date: 1989
Number of Print Pages: 11
Number of Figures: 0
Number of Tables: 0
ISSN: 1012-8204 (Print)
eISSN: 2504-2416 (Online)
For additional information: https://www.karger.com/CID
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