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Molecules and Cells in Allergy - Original Paper

Editor's Choice - Free Access

Comparison of the Performance of Skin Prick, ImmunoCAP, and ISAC Tests in the Diagnosis of Patients with Allergy

Griffiths R.L.M. · El-Shanawany T. · Jolles S.R.A. · Selwood C. · Heaps A.G. · Carne E.M. · Williams P.E.

Author affiliations

Department of Immunology, University Hospital of Wales, Cardiff, UK

Corresponding Author

Correspondence to: Dr. Paul E. Williams

Department of Immunology, University Hospital of Wales

Heath Park

Cardiff CF14 4XW (UK)

E-Mail williamspe@cardiff.ac.uk

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Int Arch Allergy Immunol 2017;172:215-223

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Background: Allergy is diagnosed from typical symptoms, and tests are performed to incriminate the suspected precipitant. Skin prick tests (SPTs) are commonly performed, inexpensive, and give immediate results. Laboratory tests (ImmunoCAP) for serum allergen-specific IgE antibodies are usually performed more selectively. The immuno-solid phase allergen chip (ISAC) enables testing for specific IgE against multiple allergen components in a multiplex assay. Methods: We retrospectively analysed clinic letters, case notes, and laboratory results of 118 patients attending the National Adult Allergy Service at the University Hospital of Wales who presented diagnostic difficulty, to evaluate which testing strategy (SPT, ImmunoCAP, or ISAC) was the most appropriate to use to confirm the diagnosis in these complex patients, evaluated in a “real-life” clinical service setting. Results: In patients with nut allergy, the detection rates of SPTs (56%) and ISAC (65%) were lower than those of ImmunoCAP (71%). ISAC had a higher detection rate (88%) than ImmunoCAP (69%) or SPT (33%) in the diagnosis of oral allergy syndrome. ImmunoCAP test results identified all 9 patients with anaphylaxis due to wheat allergy (100%), whereas ISAC was positive in only 6 of these 9 (67%). Conclusions: In this difficult diagnostic group, the ImmunoCAP test should be the preferred single test for possible allergy to nuts, wheat, other specific foods, and anaphylaxis of any cause. In these conditions, SPT and ISAC tests give comparable results. The most useful single test for oral allergy syndrome is ISAC, and SPT should be the preferred test for latex allergy.

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Article / Publication Details

First-Page Preview
Abstract of Molecules and Cells in Allergy - Original Paper

Received: January 09, 2017
Accepted: February 21, 2017
Published online: April 29, 2017
Issue release date: June 2017

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 1

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

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