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Detection of Inflammation- and Neoplasia-Associated Alterations in Human Large Intestine Using Plant/Invertebrate Lectins, Galectin-1 and Neoglycoproteins

Brinck U.a · Korabiowska M.b · Bosbach R.a · Gabius H.-J.c

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aDepartment of Gastroenterologic Pathology and bCenter of Pathology, Faculty of Medicine, University of Göttingen, and cInstitute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany

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Acta Anat 1998;161:219–233

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: October 12, 1998
Issue release date: 1998

Number of Print Pages: 15
Number of Figures: 5
Number of Tables: 5

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO

Abstract

Commonly, plant and invertebrate lectins are accepted glycohistochemical tools for the analysis of normal and altered structures of glycans in histology and pathology. Mammalian lectins and neoglycoproteins are recent additions to this panel for the detection of lectin-reactive carbohydrate epitopes and glycoligand-binding sites. The binding profiles of these three types of probes were comparatively analyzed in normal, inflamed and neoplastic large intestine. In normal colonic mucosa the intracellular distribution of glycoconjugates and carbohydrate ligand-binding sites in enterocytes reveals a differential binding of lectins with different specificity and of neoglycoproteins to the Golgi apparatus, the rough and smooth endoplasmic reticulum and the apical cell surface. The accessible glycoligand-binding sites and the lectin-reactive carbohydrate epitopes detected by galectin-1 show the same pattern of intracellular location excluding the apical cell surface. Lectin-reactive carbohydrate epitopes detected by plant lectins of identical monosaccharide specificity as the endogenous lectin [Ricinus communis agglutinin-I (RCA-I), Viscum album agglutinin (VAA)], however, clearly differ with respect to their intracellular distribution. Maturation-associated differences and heterogeneity in glycohistochemical properties of epithelial cells and non-epithelial cells (macrophages, dendritic cells, lymphocytes) are found. Dissimilarities in the fine structural ligand recognition of lectins with nominal specificity to the same monosaccharide have been demonstrated for the galactoside-specific lectins RCA-I, VAA and galectin-1 as well as the N-acetylgalactosamine (GalNAc)-specific lectins Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA) and Helix pomatia agglutinin in normal mucosa and in acute appendicitis. Acute inflammation of the intestinal mucosa found in acute phlegmonous appendicitis is associated with selective changes of glycosylation of mucin in goblet cells mainly of lower and middle crypt segments resulting in an increase of DBA- and SBA-binding sites in the goblet cell population. Appendicitis causes no detectable alteration of neoglycoprotein binding. In contrast, tumorigenesis of colonic adenoma is characterized by increases in lectin-reactive galactose (Gal; Gal-β1, 3-GalNAc), fucose and N-acetylglucosamine moieties and by enhanced presentation of respective carbohydrate ligand-binding capacity. This work reveals that endogenous lectins and neoglycoproteins are valuable glycohistochemical tools supplementing the well-known analytic capacities of plant lectins in the fields of gastrointestinal anatomy and gastroenteropathology.


References

  1. Abramenko, I.V., D.F. Gluzman, E.Y. Korchagina, T.V. Zemlyanukhina, N.V. Bovin (1992) Oligosaccharide-binding molecules on the surface of human hemopoietic and lymphoid cells. FEBS Lett 307: 283–286.
  2. Ahnen, D.J., G.H. Warren, L.J. Greene, J.W. Singleton, W.R. Brown (1987) Search for a specific marker of mucosal dysplasia in chronic ulcerative colitis. Gastroenterology 91: 1356–1355.
  3. André, S., C. Unverzagt, S. Kojima, X. Dong, C. Fink, K. Kayser, H.-J. Gabius (1997) Neoglycoproteins with the synthetic complex biantennary nonasaccharide or its α2, 3/α2, 6-sialylated derivatives: Their preparation, assessment of their ligand properties for purified lectins, for tumor cells in vitro, and in tissue sections, and their biodistribution in tumor-bearing mice. Bioconjug Chem 8: 845–855.
  4. Beranek, J.T., R. Masseyeff (1986) Commentary. Hyperplastic capillaries and their possible involvement in fibrosis. Histopathology 10: 543–546.
  5. Bockman, D.E. (1983) Functional histology of appendix. Arch Histol Jpn 46: 271–292.
    External Resources
  6. Boland, C.R., C.K. Montogomery, Y.S. Kim (1982) A cancer-associated mucin alteration in benign colonic polyps. Gastroenterology 82: 664–672.
    External Resources
  7. Bovin, N.V., H.-J. Gabius (1995) Polymer-immobilized carbohydrate ligands: Versatile chemical tools for biochemistry and medical sciences. Chem Soc Rev 24: 413–421.
  8. Bresalier, R.S., J.C. Byrd, L. Wang, A. Raz (1996) Colon cancer mucin; A new ligand for the β-galactoside-binding protein galectin-3. Cancer Res 56: 4354–4357.
  9. Brinck, U., R. Bosbach, M. Korabiowska, A. Schauer, H.-J. Gabius (1995) Lectin-binding sites in the epithelium of normal human appendix vermiformis and in acute appendicits. Histol Histopathol 10: 61–70.
  10. Brinck, U., R. Rosbach, M. Korabiowska, A. Schauer, H.-J. Gabius (1996) Histochemical study of expression of lectin-reactive carbohydrate epitopes and glycoligand-binding sites in normal human appendix vermiformis, colonic mucosa, acute appendicitis and colonic adenoma. Histol Histopathol 11: 919–930.
  11. Brockhausen, I., I. Schutzbach, W. Kuhns (1998) Glycoproteins and their relationship to human disease. Acta Anat 161: 36–78.
  12. Campo, E., E. Condom, A. Palacin, E. Quesada, A Cardesa (1988) Lectin binding patterns in normal and neoplastic colonic mucosa. A study in Dolichos biflorus agglutinin, peanut agglutinin, and wheat germ agglutinin. Dis Colon Rectum 31: 892–899.
    External Resources
  13. Dall’Olio, F., D. Trere (1993) Expression of α 2, 6-sialylated sugar chains in normal and neoplastic colon tissues. Detection by digoxigenin-conjugated Sambucus nigra agglutinin. Eur J Histochem 37: 257–265.
  14. Danguy, A., F. Akif, B. Pajak, H.-J. Gabius (1994) Contribution of carbohydrate histochemistry to glycobiology. Histol Histopathol 9: 155–171.
  15. Danguy, A., C. Decaestecker, F. Genten, I. Salmon, R. Kiss (1998) Applications of lectins and neoglycoconjugages in histology and pathology. Acta Anat 161: 206–218.
  16. Danguy, A., K. Kayser, N.V. Bovin, H.-J. Gabius (1995) The relevance of neoglycoconjugates for histology and pathology. Trends Glycosci Glycotechnol 7: 261–275.
  17. Doyle, R.J., M. Slifkin (eds) (1994) Lectin-Microorganism Interactions. New York, Dekker.
  18. Etzler, M.E. (1979) Lectins as probes in studies of intestinal glycoproteins and glycolipids. Am J Clin Nutr 32: 133–138.
    External Resources
  19. Filipe, M.I. (1979) Mucins in the human gastrointestinal epithelium: A review. Invest Cell Pathol 2: 195–216.
  20. Forstner, J., N. Taichman, V. Kalnins, G. Forstner (1973) Intestinal goblet cell mucus: Isolation and identification by immunofluorescence of a goblet cell glycoprotein. J Cell Sci 12: 586–602.
  21. Fucci L., A.M. Valentini, M.L. Caruso (1993) Can peanut agglutinin distinguish between pseudo and true invasion in colonic adenomas? Eur J Histochem 37: 335–344.
  22. Fuhrman, C., J. Breiter-Hahn /1984) Coincidence of endoplasmic reticulum pattern as visualized by FITC-Con A-fluorescence and electron microscopy. Histochemistry 80: 153–156.
  23. Gabius, H.-J. (1987) Endogenous lectins in tumors and the immune system. Cancer Invest 5: 39–46.
  24. Gabius, H.-J. (1988) Tumor lectinology: At the intersection of carbohydrate chemistry, biochemistry, cell biology and oncology. Angew Chem Int Ed 27: 1267–1276.
  25. Gabius, H.-J. (1991) Detection and functions of mammalian lectins – With emphasis on membrane lectins. Biochim Biophys Acta 1071: 1–18.
  26. Gabius, H.-J. (1997a) Animal lectins. Eur J Biochem 243: 543–576.
  27. Gabius, H.-J. (1997b) Concepts of tumor lectinology. Cancer Invest 15: 454–464.
  28. Gabius, H.-J., S. Bodanowitz, A. Schauer (1988) Endogenous sugar-binding proteins in human breast tissue and benign and malignant breast lesions. Cancer 61: 1125–1131.
    External Resources
  29. Gabius, H.-J., R. Brehler, A. Schauer, F. Cramer (1986) Localization of endogenous lectins in normal human breast, benign breast lesions and mammary carcinomas. Virchows Arch 52: 107–115.
  30. Gabius, H.-J., P.L. Debbage, N. Lang, W. Lange (1989) Malignant and normally developing trophoblastic cells of human placenta display different characteristics defined by histochemical and biochemical mapping of endogenous lectins. Histochemistry 92: 283–289.
    External Resources
  31. Gabius; H.-J., R. Engelhardt, F. Cramer, R. Bätge, G.A. Nagel (1985) Pattern of endogenous lectins in a human epithelial tumor. Cancer Res 45: 253–257.
    External Resources
  32. Gabius, H.-J., S. Gabius (1997) Glycosciences: Status and Perspectives. Weinheim, Chapman & Hall.
  33. Gabius, H.-J., S. Gabius, T.V. Zemlyanukhina, N.V. Bovin, U. Brinck, A. Dangui, S.S. Joshi, K. Kayser, J. Schottelius, F. Sinowatz, L.F. Tietze, F. Vidal-Vanaclocha, J.-P. Zanetta (1993) Reverse lectin histochemistry: Design and application of glycoligands for detection of cell and tissue lectins. Histol Histopathol 8: 369–383.
  34. Gabius, H.-J., T. Grote, S. Gabius, U. Brinck, L.F. Tietze (1991) Neoglycoprotein binding to colorectal tumor cells: Comparison between primary and secondary lesions. Virchows Arch 419: 217–222.
  35. Gabius, S., N. Yamazaki, W. Hanewacker, H.-J. Gabius (1990) Regulation of distribution, amount and ligand affinity of sugar receptors in human colon carcinoma cells by treatment with sodium butyrate, retinoic acid and phorbol ester. Anticancer Res 10: 1005–1012.
  36. Galanina, O.E., H. Kaltner, L.S. Khraltsova, N.V. Bovin, H.-J. Gabius (1997) Further refinement of the description of the ligand-binding characteristics for the galactoside-binding mistletoe lectin, a plant aggulutinin with immunomodulatory potency. J Mol Recognit 10: 139–147.
  37. Geyer, H., R. Geyer (1998) Strategies for glycoconjugate analysis. Acta Anat 161: 18–35.
  38. Gitler, C., M. Mogyoros, E. Calef, I. Rosenberg (1985) Lethal recognition between Entamoeba histolytica and the host tissues. Trans R Soc Trop Med Hy 79: 581–586.
    External Resources
  39. Grillon, C., M. Monsigny, C. Kieda (1990) Cell surface lectins of human granulocytes: Their expression is modulated by mononuclear cells and granulocyte/macrophage colony-stimulating factor. Glycobiology 1: 33–38.
  40. Hakomori, S. (1998) Cancer-associated glycosphingolipid antigens: Their structure, organization and function. Acta Anat 161: 79–90.
  41. Ho, S.B., N.W. Toribara, R.S. Bresalier, J.S. Kim (1988) Biochemical and other markers of colon cancer. Gastroenterol Clin North Am 17: 811–836.
    External Resources
  42. Hubert, I., A.P. Seve, P. Facy, M. Monsigny (1989) Are nuclear lectins and nuclear glycoproteins involved in the modulation of nuclear functions? Cell Differ Dev 27: 69–81.
    External Resources
  43. Irimura, T., Y. Matsushita, R.C. Sutton, D. Carralero, D.W. Ohannesian, K.R. Cleary, D.M. Ota, G.L. Nicolson, R. Lotan (1991) Increased content of an endogenous lactose-binding lectin in human colorectal carcinoma progressed to metastatic stages. Cancer Res 51: 387–393.
    External Resources
  44. Jass, J.R., Allison, S.M. Stewart, M.R. Lane (1993) Ulex europaeus agglutinin-1 binding in hereditary bowel cancer. Pathology 25: 114–119.
  45. Kaltner, H., K.S. Lips, G. Reuter, S. Lippert, F. Sinowatz, H.-J. Gabius (1997) Quantitation and histochemical localization of galectin-1 and galectin-1-reactive glycoconjugates in fetal development of bovine organs. Histol Histopathol 12: 945–960.
  46. Kaltner, H., B. Stierstorfer (1998) Animal lectins as cell adhesion molecules. Acta Anat 161: 162–179.
  47. Kayser, K., N.V. Bovin, E.Y. Korchagina, C. Zeilinger, F.-Y. Zeng, H.-J. Gabius (1994) Correlation of expression of binding sites for synthetic blood group A-, B-, and H-trisaccharides and for sarcolectin with survival of patients with bronchial carcinoma. Eur J Cancer 30A: 653–657.
  48. Kayser, K., J. Bubenzer, G. Kayser, S. Eichhorn, T.V. Zemlyanukhina, N.V. Bovin, S. André, J. Koopmann, H.-J. Gabius (1995) Expression of lectin-, interleukin-2-, and histo-blood group-binding sites in prostate cancer and its correlation to integrated optical and syntactic structure analysis. Anal Quant Cytol Histol 17: 135–142.
  49. Kayser, K., H.-J. Gabius (1997) Graph theory and the entropy concept in histochemistry. Prog Histochem Cytochem 32/2: 1–106.
  50. Kayser, K., M. Heil, H.-J. Gabius (1989) Is the profile of binding of a panel of neoglycoproteins useful as diagnostic marker in human lung cancer? Pathol Res Pract 184: 621–629.
    External Resources
  51. Kopitz, J., C. von Reitzenstein, M. Burchert, M. Cantz, H.-J. Gabius (1998) Galectin-1 is a major receptor for ganglioside GM1, a product of the growth-controlling activity of a cell surface ganglioside sialidase, on human neuroblastoma cells in culture. J Biol Chem 273: 11205–11211.
  52. Laine, R.A. (1997) The information-storing potential of he sugar code; in Gabius H-J, S Gabius (eds): Glycosciences: Status and Perspectives. Weinheim, Chapman & Hall, pp 1–14.
  53. Laurila, P., J. Virtanen, J. Wartiovaara, S. Stenman (1978) Fluorescent antibodies and lectins stain intracellular structures in fixed cells treated with nonionic detergent. J Histochem Cytochem 26: 251–257.
    External Resources
  54. Lee, R.T., H.-J. Gabius, Y.C. Lee (1992) Ligand binding characteristics of the major misteletoe lectin. J Biol Chem 267: 23722–23727.
  55. Lee, R.T., H.-J. Gabius, Y.C. Lee (1994) The sugar-combining area of galactose-specific toxic lectin of mistletoe extends beyond the terminal sugar residue: Comparison with a homologous toxic lectin, ricin. Carbohydr Res 254: 269–276.
  56. Lee, R.T., Y.C. Lee (1997) Neoglycoconjugates; in Gabius H-J, S Gabius (eds): Glycosciences: Status and Perspectives. Weinheim, Chapman & Hall, pp 55–77.
  57. Lee, Y. (1987) Lectin reactivity in human large bowel. Pathology 19: 397–401.
    External Resources
  58. Lee, YC, Lee RT (eds) (1994) Neoglycoconjugates. Preparation and Applications. Orlando, Academic Press.
  59. Lee, Y.S. (1988) Lectin expression in neoplastic and non-neoplastic lesions of the rectum. Pathology 20: 157–165.
    External Resources
  60. Mann, P.L., R.E. Waterman (1998) Glycocoding as an information management system in embryonic development. Acta Anat 161: 153–161.
  61. McGarrity, T.J., L.P. Pfeifer, A.B. Abt (1989) Lectin histochemistry of adenomatous polyps. Not a predictor of metachronous lesions. Cancer 64: 170–1713.
  62. Neutra, M.R., T.L. Philips, E.L. Mayer, D.J. Fishkind (1987) Transport of membrane-bound macromolecules by M cells in follicle-associated epithelium of rabbit Peyer’s patch. Cell Tissue Res 247: 537–546.
    External Resources
  63. Ofek, I., N. Sharon (1988) Lectinophagocytosis: A molecular mechanism of recognition between cell surface sugars and lectins in the phagocytosis of bacteria. Infect Immunol 56: 539–547.
  64. Ohannesian, D.W., D. Lotan, P. Thomas, J.M. Jessup, M. Fukuda, H.-J. Gabius, R. Lotan (1995) Carcinoembryonic antigen and other glycoconjugates act as ligands for galectin-3 in human colon carcinoma cells. Cancer Res 55: 2191–2199.
    External Resources
  65. Orntoft, T.F., N.C. Langkilde, H. Wiener, P.D. Ottosen (1991) Cellular localization of PNA binding in colorectal adenomas: Comparison with differentiation, nuclear: Cell height Ratio and effect of desialylation. APMIS 99: 274–281.
  66. Ota H., J. Nakayama, T. Katsuyama, M. Kanai (1988) Histochemical comparison of specificity of three bowel carcinoma-reactive lectins, Griffonia simplicifolia agglutinin, peanut agglutinin and Ulex europaeus agglutinin-I. Acta Pathol Jpn 38: 1547–1549.
    External Resources
  67. Petrie, W.A., R.D. Smith, P.H. Schlesinger, C.F. Murphy, J.L. Ravidin (1987) Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. J Clin Invest 80: 1238–1244.
  68. Plendl, J., F. Sinowatz (1998) Glycobiology of the olfactory system. Acta Anat 161: 234–253.
  69. Podolsky, D.K. (1989) The colonic goblet cell and glycoprotein heterogeneity. Immunol Invest 18: 485–497.
    External Resources
  70. Radzun, H.J., M.R. Parwaresch, A.C. Feller, M.L. Hansmann (1984) Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells. Am J Pathol 117: 441–450.
    External Resources
  71. Rhodes, J.M., R.R. Black, A. Savage (1986) Glycoprotein abnormalities in colonic carcinomata, adenomata, and hyperplastic polyps shown by lectin peroxidase histochemistry. J Clin Pathol 39: 1331–1334.
    External Resources
  72. Rüdiger, H. (1998) Plant lectins – more than just tools for glycoscientists. Occurrence, structure and possible functions of plant lectins. Acta Anat 161: 130–152.
  73. Sata, T., J. Roth, C. Zuber, B. Stamm, S.J. Rinderle, I.J. Goldstein, P.U. Heitz (1992) Studies on the Thomsen-Friedenreich antigen in human colon with the lectin Amaranthin. Normal and neoplastic epithelium express only cryptic T antigen. Lab Invest 66: 175–186.
  74. Schneller, M., S. André, J. Cihak, H. Kaltner, H. Merkle, G.J. Rademaker, J. Haverkamp, J. Thomas-Oates, U. Lösch, H.-J. Gabius (1995) Differential binding of two chicken β-galactoside-binding lectins to homologous lymphocyte subpopulations and evidence for inhibitory activity of the dimeric lectin on stimulated T cells. Cell Immunol 166: 35–43.
    External Resources
  75. Schoeppner, H.L., A. Raz, S.B. Ho, R.S. Bresalier (1994) Expression of an endogenous galactose-binding lectin correlates with neoplastic progression in the colon. Cancer 75: 2818–2826.
  76. Sharma, A., R.A. DiCioccio, H.J. Allen (1992) Identification and synthesis of a novel 15 kDa β-galactoside-binding lectin in human leukocytes. Glycobiology 2: 285–292.
  77. Sharon, N. (1987) Bacterial lectins, cell-ceel recognition and infectious disease. FEBS Lett 217: 145–157.
  78. Sharon, N. (1998) Glycoproteins now and then. Acta Anat 161: 7–17.
  79. Smetana, K., Jr., Slavik, E. Vancova, I. Fischer, F.-T. Liu, M. Burchert, X. Dong, H.-J. Gabius (1998) Fusion of macrophages on an implant surface is associated with down-regulated expression of ligands for galectin-1 and -3 in the rat. Biomaterials, in press.
  80. Solís, D., A. Romero, H. Kaltner, H.-J. Gabius, T. Díaz-Mauriño (1996) Different architecture of the combining site of the two chicken galectins revealed by chemical mapping studies with synthetic ligand derivatives. J Biol Chem 271: 12744–12748.
  81. Varki, A. (1994) Selectin ligands. Proc Natl Acad Sci USA 91: 7390–7397.
  82. Villalobo, A., H.-J. Gabius (1998) Signaling pathways for transduction of the initial message of the glycocode into cellular response. Acta Anat 161: 110–129.
  83. von der Lieth, C.-W., H.-C. Siebert, T. Kozár, M. Burchert, M. Frank, M. Gilleron, H. Kaltner, G. Kayser, E. Tajkhorshid, N.V. Bovin, J.F.G. Vliegenhart, H.-J. Gabius (1998) Lectin Ligands: New insights into their conformations and their dynamic behavior and the discovery of conformer selection by lectins. Acta Anat 161: 91–109.
  84. von Rosen, L., B. Podjaski, I. Bettman, H.F. Ott (1981) Observations on the ultrastructure and function of the so-called ‘microfold’ or ‘membranous’ cells (M cells) by means of peroxidase as a tracer. An experimental study with special attention to the physiological parameters of resorption. Virchows Arch 390: 289–312.
  85. Vyakarnam, A., S.F. Dagher, J.L. Wang, R.J. Patterson (1997) Evidence for a role for galectin-1 in pre-mRNA splicing. Mol Cell Biol 17: 4730–4737.
    External Resources
  86. Wasano, K., Y. Hirakawa (1997) Recombinant galectin-1 recognizes mucin and epithelial cell surface glycocalyces of gastrointestinal tract. J Histochem Cytochem 45: 275–283.
  87. Wolf, J.L., W.A. Bye (1984) The membranous epithelial (M) cell and the mucosal immune system. Annu Rev Med 35: 95–112.
  88. Yoshioka, H., M. Inada, K. Ogawa, G. Ohshio, H. Yamabe, Y. Hamashima, T. Miyake (1989) Lectin histochemistry in ulcerative colitis and Crohn disease. J Exp Pathol 4: 69–78.
    External Resources
  89. Zanetta, J.-P. (1998) Structure and functions of lectins in the central and peripheral nervous system. Acta Anat 161: 180–195.
  90. Zschäbitz, A. (1998) Glycoconjugate expression and cartilage development in the cranial skeleton. Acta Anat 161: 254–274.

Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: October 12, 1998
Issue release date: 1998

Number of Print Pages: 15
Number of Figures: 5
Number of Tables: 5

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: https://www.karger.com/CTO


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