Cellular Distribution of Platelet-Derived Growth Factor, Transforming Growth Factor-β, Basic Fibroblast Growth Factor, and Their Receptors in Normal Bone MarrowYoon S.-Y.b · Tefferi A.a · Li C.-Y.b
Divisions of aHematology and Internal Medicine and bHematopathology, Mayo Clinic and Mayo Foundation, Rochester, Minn., USA
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We performed an immunohistochemical study on 5 normal marrow samples and 3 fibrotic marrow samples to investigate the cellular distribution of various isoforms of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and their corresponding receptors. Immature hematopoietic precursors strongly expressed PDGF-B, all the TGF-β isoforms, PDGFRβ, TGFβRI and II, and FGFR1, 3, and 4. Megakaryocytes stained primarily for PDGF-B, TGF-β2–3, and PDGFRβ. Histiocytes displayed intense TGF-β1, bFGF, and FGFR2 expression. Fibroblasts and endothelial cells carried receptors for all the aforementioned cytokines. The last 2 cell types also expressed the ligand cytokines to varied degrees.
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