Acta Haematologica

 

Immune Reconstitution following Transplantation of Autologous Peripheral CD34+ Cells

Sica S. · Laurenti L. · Sorà F. · Menichella G. · Rumi C. · Leone G. · Rutella S.

Author affiliations

Department of Haematology, Catholic University School of Medicine, Rome, Italy

Keywords: Immune reconstitutionAutologous transplantationImmunoselected CD34+ progenitors

Related Articles for ""
Acta Haematol 2001;105:179–187
https://doi.org/10.1159/000046562

Log in to MyKarger to check if you already have access to this content.




Forgot your password
Sign up to MyKarger

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more
CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!


If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates

Select
* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: July 18, 2001
Issue release date: July 2001

Number of Print Pages: 9
Number of Figures: 2
Number of Tables: 3

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: https://www.karger.com/AHA

Abstract

The recovery of lymphocyte count, CD4+ and CD8+ T-cell subsets, natural killer (NK) cells and CD19+ B cells has been evaluated during the first 4 months after the infusion of autologous CD34+ peripheral blood progenitor cells (PBPC; group A; 33 patients) or autologous unselected PBPC (group B; 36 patients) for hematological malignancies. Lymphocyte count promptly recovered in both patient cohorts, although the repopulation of CD3+ T cells occurred more rapidly in group B compared with group A. The count of CD4+ T lymphocytes remained <200/µl during the study period in patients transplanted with CD34+ PBPC, being significantly lower compared with group B (p = 0.0019 and p = 0.0035 on days 30 and 60, respectively). CD8+ T cells rapidly increased both in group A and B and CD4 to CD8 ratio was severely reduced. CD4+ and CD8+ T cells displayed an activated phenotype in both groups of patients, coexpressing the HLA-DR antigen throughout the study period. No differences in the repopulation kinetics of NK cells and CD19+ B cells were observed. Further investigations are encouraged to characterize T cell competence following transplantation of CD34+ PBPC.

© 2001 S. Karger AG, Basel



Related Articles:

References

  1. Schmitz N, Bacigalupo A, Labopin M, Majolino I, Laporte J, Brinch L, Cook G, Lambertenghi Deliliers G, Lange A, Rozman C, Garcia-Conde J, Finke J, Domingo-Albos A, Gratwohl A: Transplantation of peripheral blood progenitor cells from HLA-identical sibling donors. Br J Haematol 1996;95:715–723.
    External Resources
  2. Jin NR, Lum LG, Buren EV, Lerman SP, Walker EL, June CH: Signal transduction by B and T cells early after bone marrow transplantation: B cells calcium flux responses are intact whereas lack of CD4 cells accounts for impaired T cell responses. Bone Marrow Transplant 1995;16:103–109.
    External Resources
  3. Talmadge JE, Reed E, Ino K, Kessinger A, Kuszynski C, Heimann D, Varney M, Jackson J, Vose JM, Bierman PJ: Rapid immunologic reconstitution following transplantation with mobilized peripheral blood stem cells as compared to bone marrow. Bone Marrow Transplant 1997;19:161–172.
    External Resources
  4. Storek J, Witherspoon RP, Storb R: T cell reconstitution after bone marrow transplantation into adult patients does not resemble T cell development in early life. Bone Marrow Transplant 1995;16:413–425.
    External Resources
  5. Ottinger HD, Beelen DW, Scheulen B, Schaefer UW, Grosse Wilde H: Improved immune reconstitution after allotransplantation of peripheral blood stem cells instead of bone marrow. Blood 1996;88:2775–2779.
    External Resources
  6. Koehne G, Zeller W, Stockschlaeder M, Zander AR: Phenotype of lymphocyte subsets after autologous peripheral blood stem cell transplantation. Bone Marrow Transplant 1997;19:149–156.
    External Resources
  7. Fegan C, Thomas H, Bailey-Wood R, Coleman S, Phillips S, Hoy T, Whittekar JA: In vitro LAK activity following autologous peripheral blood stem cell is significantly greater than that following autologous bone marrow and allogeneic bone marrow transplantation. Bone Marrow Transplant 1995;16:277–281.
    External Resources
  8. Hebib NC, Deas O, Rouleau M, Durrbach A, Charpentier B, Beaujean F, Vernant JP, Senik A: Peripheral blood T cells generated after allogeneic bone marrow transplantation: Lower levels of bcl-2 protein and enhanced sensitivity to spontaneous and CD95-mediated apoptosis in vitro. Abrogation of the apoptotic phenotype coincides with the recovery of normal naive/primed T cell profiles. Blood 1999;94:1803–1813.
    External Resources
  9. Lin M, Tseng L, Frangoul H, Gooley T, Pei J, Barsoukov A, Akatsuka Y, Hansen JA: Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation. Blood 2000;95:3832–3839.
    External Resources
  10. Hakim FT, Cepeda R, Kaimei S, Mackall CL, McAtee N, Zujewski JA, Cowan K, Gress RE: Constraints on CD4 recovery postchemotherapy in adults: Thymic insufficiency and apoptotic decline of expanded peripheral CD4 cells. Blood 1997;90:3789–3798.
    External Resources
  11. Guillaume T, Rubinstein DB, Symann M: Immune reconstitution and immunotherapy after autologous hematopoietic stem cell transplantation. Blood 1998;92:1471–1490.
    External Resources
  12. Laurenti L, Sica S, Sorà F, Piccirillo N, Ortu La barbera E, Chiusolo P, Salutari P, Rumi C, Rutella S, Leone G: Long-term immune recovery after CD34+ immunoselected and unselected peripheral blood progenitor cell transplantation: A case-control study. Haematologica 1999;84:1100–1103.
    External Resources
  13. Tricot G, Gazitt Y, Leemhuis T, Jagannath S, Desikan KR, Siegel D, Fassas, A, Tindle S, Nelson J, Juttner C, Tsukamoto A, Hallagan J, Atkinson K, Reading C, Hoffman R, Barlogie B: Collection, tumor contamination and engraftment kinetics of highly purified hematopoietic progenitor cells to support high dose therapy in multiple myeloma. Blood 1998;91:4489–4495.
    External Resources
  14. Vescio R, Schiller G, Stewart AK, Ballester O, Noga S, Rugo H, Freytes C, Stadtmauer E, Tarantolo S, Sahebi F, Stiff P, Meharchard J, Schlossman R, Brown R, Tully H, Benyunes M, Jacobs C, Berenson R, Di Persio J, Anderson K, Berenson J: Multicenter phase III trial to evaluate CD34+ selected versus unselected autologous peripheral blood progenitor cell transplantation in multiple myeloma. Blood 1999;93:1858–1868.
    External Resources
  15. Bomberger C, Singh-Jairam M, Rodey G, Guerriero A, Yeager AM, Fleming WH, Holland HK, Waller EK: Lymphoid reconstitution after autologous PBSC transplantation with FACS-sorted CD34+ hematopoietic progenitors. Blood 1998;91:2588–2600.
    External Resources
  16. Diviné M, Boutolleau D, Delfau-Larue MH, Beaujean F, Jouault H, Reyes F, Kuentz M, Bensussan A, Farcet JP, Boumsell L: Poor lymphocyte recovery following CD34-selected autologous peripheral blood stem cell transplantation for non-Hodgkin’s lymphoma. Br J Haematol 1999;105:349–360.
    External Resources
  17. Douek DC, Vescio RA, Betts MR, et al: Assessment of thymic output in adults after haematopoietic stem cell transplantation and prediction of T cell reconstitution. Lancet 2000;355:1875–1881.
    External Resources
  18. Rutella S, Rumi C, Laurenti L, Pierelli L, Sorà F, Sica S, Leone G: Immune reconstitution following transplantation of autologous peripheral CD34+ cells: Analysis of predictive factors and comparison with unselected progenitor transplants. Br J Haematol 2000;108:105–115.
    External Resources
  19. Martinez C, Urbano-Ispizua A, Rozman C, Marin P, Rovira M, Sierra J, Montfort N, Carreras E, Montserrat E: Immune reconstitution following allogeneic peripheral blood progenitor cell transplantation: Comparison of recipients of positive CD34+ selected grafts with recipients of unmanipulated grafts. Exp Hematol 1999;27:561–568.
    External Resources
  20. MacDonald HR, Blanc C, Lees RK, Sordat B: Abnormal distinction of T cell subsets in athymic mice. J Immunol 1986;136:4337–4339.
    External Resources
  21. Pavletic ZS, Joshi SS, Pirruccello SJ, Tarantolo SR, Kollath J, Reed EC, Bierman PJ, Vose JM, Warkentin PI, Gross TG, Nasrati K, Armitage JO, Kessinger A, Bishop MR: Lymphocyte reconstitution after allogeneic blood stem cell transplantation for hematologic malignancies. Bone Marrow Transplant 1998;21:33–41.
    External Resources
  22. Laurenti L, Sica S, Salutari P, Rutella S, Serafini R, D’Onofrio G, Rumi C, Leone G: Assessment of hematological and immunological function during long-term follow-up after peripheral blood stem cell transplantation. Haematologica 1998;83:138–142.
    External Resources
  23. Leino L, Lilius EM, Nikoskelainen J, Pelliniemi TT, Rajamaki A: The reappearance of 10 differentiation antigens on peripheral blood lymphocytes after allogeneic bone marrow transplantation. Bone Marrow Transplant 1991;8:339–344.
    External Resources
  24. Miyamoto T, Gondo H, Miyoshi Y, Shigematsu H, Minematsu T, Takenaka K, Tanimoto K, Horiuchi T, Asano Y, Inaba S, Minamishima Y, Niho Y: Early viral complications following CD34-selected autologous peripheral blood stem cell transplantation for non-Hodgkin’s lymphoma. Br J Haematol 1998;100:348–350.
    External Resources
  25. Small TN, Papadopoulos EB, Boulad F, Black P, Castro-Malaspina H, Childs BH, Collins N, Gillio A, George D, Jakubowski A, Heller G, Fazzari M, Kernan N, MacKinnon S, Szabolcs P, Young JW, O’Reilly RJ: Comparison of immune reconstitution after unrelated and related T-cell-depleted bone marrow transplantation: effect of patient age and donor lymphocyte infusions. Blood 1999;93:467–480.
    External Resources
  26. Sica S, Sorà F, Chiusolo P, Laurenti L, Cattani P, Giordano G, Piccirillo N, Leone G: Early viral complications after autologous CD34-selected peripheral blood stem cell transplantation. Bone Marrow Transplant 2000;26:587–588.
    External Resources
  27. Holmberg LA, Boeckh M, Hooper H, Leisenring W, Rowley S, Heimfeld S, Press O, Maloney DG, McSweeney P, Corey L, Mariarz RT, Appelbaum FR, Bensinger W: Increased incidence of cytomegalovirus disease after autologous CD34-selected peripheral blood stem cell transplantation. Blood 1999;94:4029–4035.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: July 18, 2001
Issue release date: July 2001

Number of Print Pages: 9
Number of Figures: 2
Number of Tables: 3

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: https://www.karger.com/AHA

Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Article Tools
Article Details

2001, Vol.105, No. 3

July 2001

Article

Recommend This
TOP