Gynecologic and Obstetric Investigation

Original Article

Increased Toll-Like Receptor-Myeloid Differentiation Factor 88 Expression at the Maternal-Fetal Interface Is Associated with Spontaneous Abortion

He M.a · Zhou Y.a, b · Jiang M.a, c · Li F.a · Yang M.a · Fan Y.a · Deng D.a

Author affiliations

aDepartment of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, bDepartment of Reproductive Medical Center, Tangdu Hospital, The Fourth Military Medical University, Xi'an, and cDepartment of Gynecology and Obstetrics, Northern Jiangsu Province Hospital, Clinical Medical College, Yangzhou University, Yangzhou, PR China

Keywords: Recurrent spontaneous abortionToll-like receptorsMyeloid differentiation factor 88Innate immune systemMaternal–fetal interface

Related Articles for ""
Gynecol Obstet Invest 2017;82:553–562
https://doi.org/10.1159/000468931

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: July 04, 2016
Accepted: March 06, 2017
Published online: June 14, 2017
Issue release date: November 2017

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 1

ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)

For additional information: https://www.karger.com/GOI

Abstract

Aims: Toll-like receptors (TLRs) form a group of pattern recognition receptors that play a major role in maintaining innate host immunity. Myeloid differentiation factor 88 (MyD88) is an adaptor molecule that is essential for signaling via the TLR family. To analyze the mechanism of the imbalance in the innate immune system mediated by TLRs-MyD88 in spontaneous abortion, we detected the expression of TLR-2, TLR-4, TLR-7, and MyD88 in placentae and deciduas. Methods: The expression of TLR-2, TLR-4, TLR-7, and MyD88 in the placentae and deciduas of abortion-prone mice (n = 10) and normal pregnant mice (n = 10) were analyzed by immunochemistry, western blot, and real-time polymerase chain reaction. Results: There were higher protein expression levels of TLR-2, TLR-4, TLR-7, and MyD88 in the placentae and deciduas in the abortion-prone group than in the normal pregnant group. However, TLR-2, TLR-4, and TLR-7 showed lower gene expression, while MyD88 manifested higher gene expression in placentae and deciduas of abortion-prone mice matings. Conclusions: TLR-2, TLR-4, TLR-7, and MyD88 were expressed in the placenta and decidua of both the abortion-prone pregnant and normal pregnant mice. The overexpression of TLRs may excessively activate the innate immune system mediated by MyD88, which is considered to be related to the pathogenesis of spontaneous abortion.

© 2017 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: July 04, 2016
Accepted: March 06, 2017
Published online: June 14, 2017
Issue release date: November 2017

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 1

ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)

For additional information: https://www.karger.com/GOI

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2017, Vol.82, No. 6

November 2017

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M.H. and Y.Z. contributed equally to this work.
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