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Characteristics of Human Intestinal Acid SialidaseTandt W. R. D.a · Adriaenssens K.b · Scharpé S.a(a)Department of Medicine, Universitaire Instelling Antwerpen, Wilrijk, Belgium;
(b) Provincial Institute, Antwerp, Belgium
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Article / Publication Details
Published online: August 11, 2017
Issue release date: 1987
Number of Print Pages: 4
Number of Figures: 0
Number of Tables: 0
ISSN: 0013-9432 (Print)
eISSN: 2504-2564 (Online)
For additional information: https://www.karger.com/EZD
Abstract
With methylumbelliferyl-N-acetyl-neuraminic acid (MU-NANA) as substrate, acid sialidase was determined in intestinal biopsies of children. The enzyme has an acid pH optimum, a K(m) value of 4 mmol/1 and a pronounced thermal lability which can be partially prevented by the addition of albumin. N-acetyl-neuraminic acid (NANA) and derivatives as well as other glycoprotein and oligosaccharide sialidase substrates inhibit sialidase whereas gangliosides have no effect. This could be an indication that intestinal MU-NANA sialidase is different from ganglioside sialidase as has been reported for many other tissues.
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Article / Publication Details
Published online: August 11, 2017
Issue release date: 1987
Number of Print Pages: 4
Number of Figures: 0
Number of Tables: 0
ISSN: 0013-9432 (Print)
eISSN: 2504-2564 (Online)
For additional information: https://www.karger.com/EZD
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