European Urology
Research Paper
Immunohistochemical Studies on the Expression of HLA Class I Antigens in Renal Cell Carcinoma: Comparison of Primary and Metastatic Tumor TissueBuszello H. · Ackermann R.Department of Urology, Heinrich Heine University, Düsseldorf, FRG
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Article / Publication Details
Published online: August 11, 2017
Issue release date: 1994
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 0302-2838 (Print)
eISSN: 1873-7560 (Online)
For additional information: https://www.karger.com/EUR
Abstract
HLA class I expression was studied in 43 specimens of normal renal tissue, 48 primary renal cell carciomas (RCCs), and 12 métastasés by immunofluorescence, using two different monoclonal antibodies (MoAbs; IOT2c, W6/32). Homogenous staining of all normal renal tissues was observed, as expected. From the panel of 48 renal tumors, 40 had a strong expression of HLA class I antigens when tested with both MoAbs, 7 tumors had only weak expression of HLA class I and were regarded as 'HLA class I negative', and 1 tumor showed homogenous staining when tested with one MoAb (IOT2c) but was negative when the other was used (W6/32). This was the only case in which different results with the two MoAbs were found. Reduced expression of HLA class I antigens was observed in 5/12 métastasés, and in 1 metastasis no expression of HLA class I could be seen. The staining chartacteristics of 10 métastasés in comparison with the respective primary tumors were as follows: same HLA class I score 7/10; loss of HLA class I 3/10; gain of HLA class 10/10. In RCC, a complete loss of HLA class I expression is a rare phenomena, which was observed in 0/48 primary tumors and in 1/12 métastasés. Reduced expression of HLA class I antigens occurs in about 15% of primary tumors but is more frequent (50%) in métastasés; however, if this is relevant for cytotoxicity by T lymphocytes is unknown.
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Article / Publication Details
Published online: August 11, 2017
Issue release date: 1994
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 0302-2838 (Print)
eISSN: 1873-7560 (Online)
For additional information: https://www.karger.com/EUR
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