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Original Paper

Open Access Gateway

Impact of a pH 5 Oil-in-Water Emulsion on Skin Surface pH

Fürtjes T.a · Weiss K.T.a · Filbry A.b · Rippke F.b · Schreml S.a

Author affiliations

aDepartment of Dermatology, University Medical Center Regensburg, Regensburg, and bBeiersdorf AG, Hamburg, Germany

Corresponding Author

Stephan Schreml, MD

Department of Dermatology, University Medical Center Regensburg

Franz-Josef-Strauss-Allee 11

DE-93053 Regensburg (Germany)

E-Mail stephan@schreml.de

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Skin Pharmacol Physiol 2017;30:292-297

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Background: Human skin surface has a physiologically acidic pH (pHss). In cases of increased pHss, the acidity of the skin can be restored by topical formulations. We tested a pH 5 oil-in-water (O/W) emulsion for pHss regeneration and stabilization. Methods: We performed 2 experiments with 10 female study subjects in each. In both experiments, 2D imaging with luminescent sensor foils was used to determine pHss. Alkalization was reached by washing the volar forearm with a soap bar and warm running tap water for 20 min. Experiment 1: after defining the baseline pHss, we alkalized the respective area and measured pHss over a duration of 5 h, while applying emulsion every hour. Experiment 2: study subjects used the emulsion twice daily for 1 week. Then, pHss was measured before and after 5 min of washing a treated and an untreated area on the volar forearm. Results: (1) 5 h after alkalization, the treated arm showed a significantly lower pHss than the untreated one (5.87 ± 0.03 vs. 6.05 ± 0.03); (2) after washing, the treated area had a significantly lower pHss than controls (6.13 ± 0.03 vs. 6.27 ± 0.05). Conclusions: The tested pH 5 O/W emulsion seems to improve regeneration and stabilization of pHss.

© 2017 The Author(s) Published by S. Karger AG, Basel


  1. Lambers H, Piessens S, Bloem A, Pronk H, Finkel P: Natural skin surface pH is on average below 5, which is beneficial for its resident flora. Int J Cosmet Sci 2006;28:359-370.
  2. Schreml S, Meier RJ, Wolfbeis OS, Landthaler M, Szeimies R-M, Babilas P: 2D luminescence imaging of pH in vivo. Proc Natl Acad Sci USA 2011;108:2432-2437.
  3. Öhman H, Vahlquist A: In vivo studies concerning a pH gradient in human stratum corneum and upper epidermis. Acta Derm Venereol 1994;74:375-379.
    External Resources
  4. Behne MJ, Meyer JW, Hanson KM, Barry NP, Murata S, Crumrine D, Clegg RW, Gratton E, Holleran WM, Elias PM, Mauro TM: NHE1 regulates the stratum corneum permeability barrier homeostasis. Microenvironment acidification assessed with fluorescence lifetime imaging. J Biol Chem 2002;277:47399-47406.
  5. Fluhr JW, Kao J, Jain M, Ahn SK, Feingold KR, Elias PM: Generation of free fatty acids from phospholipids regulates stratum corneum acidification and integrity. J Invest Dermatol 2001;117:44-51.
  6. Haverkampf S, Heider J, Weiss KT, Berneburg M, Karrer S, Schreml S, Haubner F, Ettl T, Schreml J, Hedtrich S, Susskind-Schwendi M von, Dissemond J: NHE1 expression at wound margins increases time-dependently during physiological healing. Exp Dermatol 2017;26:124-126.
  7. Krien PM, Kermici M: Evidence for the existence of a self-regulated enzymatic process within the human stratum corneum -an unexpected role for urocanic acid. J Invest Dermatol 2000;115:414-420.
  8. Fluhr JW, Elias PM: stratum corneum pH: formation and function of the “acid mantle”. Exog Dermatol 2002;1:163-175.
  9. Öhman H, Vahlquist A: The pH gradient over the stratum corneum differs in X-linked recessive and autosomal dominant ichthyosis: a clue to the molecular origin of the “acid skin mantle”? J Invest Dermatol 1998;111:674-677.
  10. Takagi Y, Kaneda K, Miyaki M, Matsuo K, Kawada H, Hosokawa H: The long-term use of soap does not affect the pH-maintenance mechanism of human skin. Skin Res Technol 2015;21:144-148.
  11. Elias PM: Stratum corneum defensive functions: an integrated view. J Invest Dermatol 2005;125:183-200.
  12. Schreml S, Szeimies R-M, Karrer S, Heinlin J, Landthaler M, Babilas P: The impact of the pH value on skin integrity and cutaneous wound healing. J Eur Acad Dermatol Venereol 2010;24:373-378.
  13. Hachem J-P, Crumrine D, Fluhr J, Brown BE, Feingold KR, Elias PM: pH directly regulates epidermal permeability barrier homeostasis, and stratum corneum integrity/cohesion. J Invest Dermatol 2003;121:345-353.
  14. Schmid-Wendtner M-H, Korting HC: The pH of the skin surface and its impact on the barrier function. Skin Pharmacol Physiol 2006;19:296-302.
  15. Schreml S, Kemper M, Abels C: Skin pH in the elderly and appropriate skin care. Eur Med J Dermatol 2014;86-94.
  16. Ananthapadmanabhan KP, Moore DJ, Subramanyan K, Misra M, Meyer F: Cleansing without compromise: the impact of cleansers on the skin barrier and the technology of mild cleansing. Dermatol Ther 2004;17(suppl 1):16-25.
  17. Rippke F, Schreiner V, Schwanitz H-J: The acidic milieu of the horny layer: new findings on the physiology and pathophysiology of skin pH. Am J Clin Dermatol 2002;3:261-272.
  18. Wilhelm KP, Maibach HI: Factors predisposing to cutaneous irritation. Dermatol Clin 1990;8:17-22.
    External Resources
  19. Behm B, Kemper M, Babilas P, Abels C, Schreml S: Impact of a glycolic acid-containing pH 4 water-in-oil emulsion on skin pH. Skin Pharmacol Physiol 2015;28:290-295.
  20. Ali SM, Yosipovitch G: Skin pH: from basic science to basic skin care. Acta Derm Venereol 2013;93:261-267.
  21. Blaak J, Wohlfart R, Schürer NY: Treatment of aged skin with a pH 4 skin care product normalizes increased skin surface pH and improves barrier function: results of a pilot study. J Cosmet Dermatol Sci Appl 2011;01:50-58.
    External Resources
  22. Blaak J, Kaup O, Hoppe W, Baron-Ruppert G, Langheim H, Staib P, Wohlfart R, Lüttje D, Schürer NY: A long-term study to evaluate acidic skin care treatment in nursing home residents: impact on epidermal barrier function and microflora in aged skin. Skin Pharmacol Physiol 2015;28:269-279.
  23. Schreml S, Meier RJ, Albert MG, Seidl U, Zeller V, Behm B, Landthaler M, Abels C, Babilas P: The impact of 10% alpha-hydroxy acid emulsion on skin pH. Skin Pharmacol Physiol 2012;25:34-38.
  24. Meier RJ, Schreml S, Wang X-D, Landthaler M, Babilas P, Wolfbeis OS: Simultaneous photographing of oxygen and pH in vivo using sensor films. Angew Chem Int Ed Engl 2011;50:10893-10896.
  25. Voegeli D: The effect of washing and drying practices on skin barrier function. J Wound Ostomy Continence Nurs 2008;35:84-90.
  26. Gfatter R, Hackl P, Braun F: Effects of soap and detergents on skin surface pH, stratum corneum hydration and fat content in infants. Dermatology 1997;195:258-262.
    External Resources
  27. Luebberding S, Kolbe L, Kerscher M: Influence of sportive activity on skin barrier function: a quantitative evaluation of 60 athletes. Int J Dermatol 2013;52:745-749.
  28. Korting HC, Hübner K, Greiner K, Hamm G, Braun-Falco O: Differences in the skin surface pH and bacterial microflora due to the long-term application of synthetic detergent preparations of pH 5.5 and pH 7.0. Results of a crossover trial in healthy volunteers. Acta Derm Venereol 1990;70:429-431.
    External Resources
  29. Schreml S, Zeller V, Meier RJ, Korting HC, Behm B, Landthaler M, Babilas P: Impact of age and body site on adult female skin surface pH. Dermatology 2012;224:66-71.
  30. Williams S, Davids M, Reuther T, Kraus D, Kerscher M: Gender difference of in vivo skin surface pH in the axilla and the effect of a standardized washing procedure with tap water. Skin Pharmacol Physiol 2005;18:247-252.
  31. Fluhr JW, Dickel H, Kuss O, Weyher I, Diepgen TL, Berardesca E: Impact of anatomical location on barrier recovery, surface pH and stratum corneum hydration after acute barrier disruption. Br J Dermatol 2002;146:770-776.
  32. Ehlers C, Ivens UI, Moller ML, Senderovitz T, Serup J: Females have lower skin surface pH than men. A study on the surface of gender, forearm site variation, right/left difference and time of the day on the skin surface pH. Skin Res Technol 2001;7:90-94.
  33. Blaak J, Dahnhardt D, Dahnhardt-Pfeiffer S, Bielfeldt S, Wilhelm K-P, Wohlfart R, Staib P: A plant oil-containing pH 4 emulsion improves epidermal barrier structure and enhances ceramide levels in aged skin. Int J Cosmet Sci 2017;39:284-291.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 14, 2017
Accepted: August 13, 2017
Published online: October 04, 2017
Issue release date: Published online first

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 0

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: https://www.karger.com/SPP

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