Gene Expression of Neurotrophins and Their High-Affinity Trk Receptors in Cultured Human Müller CellsOku H.a · Ikeda T.a · Honma Y.b · Sotozono C.b · Nishida K.b · Nakamura Y.b · Kida T.a · Kinoshita S.b
aDepartment of Ophthalmology, Osaka Medical College, Osaka, and bDepartment of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
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Purpose: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells. Methods: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells. Results: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA. Conclusion: Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors.
© 2002 S. Karger AG, Basel
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