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Original Article

Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes)

Viana P.F.a · Ezaz T.d · Marajó L.a · Ferreira M.a · Zuanon J.a · Cioffi M.B.c · Bertollo L.A.C.c · Gross M.C.b · Feldberg E.a

Author affiliations

aInstituto Nacional de Pesquisas da Amazônia, Coordenação de Biodiversidade, and bInstituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, and cDepartamento de Genética e Evolucão, Universidade Federal de São Carlos, São Carlos, Brazil; dInstitute for Applied Ecology, University of Canberra, Canberra, ACT, Australia

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Cytogenet Genome Res 2017;153:96-104

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: August 08, 2017
Published online: November 30, 2017
Issue release date: January 2018

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family.

© 2017 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: August 08, 2017
Published online: November 30, 2017
Issue release date: January 2018

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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