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Original Article

Cytogenetic Characterization of a Population of Acanthodactylus lineomaculatus Duméril and Bibron, 1839 (Reptilia, Lacertidae), from Southwestern Morocco and Insights into Sex Chromosome Evolution

Giovannotti M.a · Nisi Cerioni P.a · Slimani T.c · Splendiani A.a · Paoletti A.a · Fawzi A.c · Olmo E.a · Caputo Barucchi V.a, b

Author affiliations

aDipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, and bIstituto di Scienze Marine Sezione Pesca Marittima, Consiglio Nazionale delle Ricerche, Ancona, Italy; cLaboratory of Biodiversity and Ecosystem Dynamics, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco

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Cytogenet Genome Res

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: August 28, 2017
Published online: November 29, 2017

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Acanthodactylus lineomaculatus is now regarded as an ecotype of A. erythrurus with which it has been recently synonymized. Despite the wide range of A. erythrurus, karyological data for this species are scarce and limited to classical cytogenetic studies carried out in individuals from only 2 locations (central Spain and Spanish enclave of Melilla on the northwestern Mediterranean Moroccan coast). Here, for the first time, we cytogenetically characterized individuals of A. lineomaculatus from the southwestern Moroccan Atlantic coast with the aim to increase the karyological knowledge of this wide-ranging species and to assess if any chromosomal changes can be found in this ecotype in comparison to other populations of this species. The diploid number of the individuals investigated is 2n = 38 which is typical of most lacertids. Active NORs were located telomerically in a medium-small pair of chromosomes, and no inactive NORs were detected. C-banding revealed an intensely heterochromatic W chromosome composed of AT-rich (centromere and long arm telomeric region) and GC-rich (most of the long arm) regions, with extended interstitial telomeric sequences. These telomere-like repeats occupy the GC-rich heterochromatin of the W. The DNA composition of the W represents a trait distinguishing A. lineomaculatus (southwestern Morocco) from A. erythrurus from Spain that possess a DAPI-positive (AT-rich) W chromosome. In conclusion, these results add further evidence to the remarkable karyotype conservation in lacertid lizards, although differences in NOR location and in W chromosome structure among populations could suggest an incipient speciation mediated by chromosome changes in this wide-ranging lizard species.

© 2017 S. Karger AG, Basel


References

  1. Altmanová M, Rovatsos M, Kratochvíl L, Johnson Pokorná M: Minute Y chromosomes and karyotype evolution in Madagascan iguanas (Squamata: Iguania: Opluridae). Biol J Linnean Soc 118:618-633 (2016).
  2. Arnold EN, Arribas O, Carranza S: Systematics of the Palaearctic and Oriental lizard tribe Lacertini (Squamata: Lacertidae: Lacertinae), with descriptions of eight new genera. Zootaxa 1430:1-86 (2007).
  3. Arribas OJ, Carranza S, Odierna G: Description of a new endemic species of mountain lizard from northwestern Spain: Iberolacerta galani sp. nov. (Squamata: Lacertidae). Zootaxa 1240:1-55 (2006).
  4. Balajee AS, Oh HJ, Natarajan AT: Analysis of restriction enzyme-induced chromosome aberrations in the interstitial telomeric repeat sequences of CHO and CHE cells by FISH. Mutat Res 307:307-313 (1994).
  5. Bons J, Geniez P: Contribution to the systematics of the lizard Acanthodactylus erythrurus (Sauria, Lacertidae) in Morocco. Herpetol J 5:271-280 (1995).
  6. Capriglione T, Cardone A, Odierna G, Olmo E: Evolution of a centromeric satellite DNA and phylogeny of lacertid lizards. Comp Biochem Physiol B 100:641-645 (1991).
  7. Caputo V, Giovannotti M, Nisi Cerioni P, Splendiani A, Olmo E: Chromosomal study of native and hatchery trouts from Italy (Salmo trutta complex, Salmonidae): conventional and FISH analysis. Cytogenet Genome Res 124:51-62 (2009).
  8. Fonseca MM, Brito JC, Paulo OS, Carretero MA, Harris DJ: Systematic and phylogeographical assessment of the Acanthodactylus erythrurus group (Reptilia: Lacertidae) based on phylogenetic analyses of mitochondrial and nuclear DNA. Mol Phylogenet Evol 51:131-142 (2009).
  9. Giovannotti M, Nisi Cerioni P, Caputo V, Olmo E: Characterisation of a GC-rich telomeric satellite DNA in Eumecesschneideri Daudin (Reptilia, Scincidae). Cytogenet Genome Res 125:272-278 (2009).
  10. Giovannotti M, Rojo V, Nisi Cerioni P, González-Tizón AM, Martínez-Lage A, et al: Isolation and characterization of two satellite DNAs in some Iberian rock lizards (Squamata, Lacertidae). J Exp Zool B Mol Dev Evol 322:13-26 (2014).
  11. Giribet G, Carranza S, Baguñà J, Ruitort M, Ribera C: First molecular evidence for the existence of a Tardigrada + Arthropoda clade. Mol Biol Evol 13:76-84 (1996).
  12. Gorman GC: The chromosomes of the Reptilia: a cytotaxonomic interpretation, in Chiarelli B, Capanna E (eds): Cytotaxonomy and Vertebrate Evolution, pp 349-424 (Academic Press, London 1973).
  13. Harris DJ, Arnold EN: Elucidation of the relationships of spiny-footed lizards, Acanthodactylus spp. (Reptilia: Lacertidae) using mitochondrial DNA sequence, with comments on their biogeography and evolution. J Zool 252:351-362 (2000).
  14. Harris DJ, Batista V, Carretero MA: Assessment of genetic diversity within Acanthodactyluserythrurus (Reptilia: Lacertidae) in Morocco and the Iberian Peninsula using mitochondrial DNA sequence data. Amphibia-Reptilia 25:227-232 (2004).
  15. Hipsley CA, Himmelmann L, Metzler D, Müller J: Integration of Bayesian molecular clock methods and fossil-based soft bounds reveals early Cenozoic origin of African lacertid lizards. BMC Evol Biol 9:151 (2009).
  16. Howell WM, Black DA: Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014-1015 (1980).
  17. King M: Chromosome change and speciation in lizards, in Atchley WR, Woodruff D (eds): Evolution and Speciation: Essays in Honor of M.J.D. White, pp 262-285 (Cambridge University Press, Cambridge 1981).
  18. Kubat Z, Hobza R, Vyskot B, Kejnovsky E: Microsatellite accumulation on the Y chromosome in Silene latifolia. Genome 51:350-356 (2008).
  19. Mateo JA, Cano J: On the karyotype of three lacertid species: Acanthodactylus erythrurus (Schinz), Lacerta schreiberi Bedriaga and Lacerta perspicillata (Dum. and Bib.). Rev Esp Herp 5:141-147 (1991).
  20. Matsubara K, Uno Y, Srikulnath K, Matsuda Y, Miller E, Olsson M: No interstitial telomeres on autosomes but remarkable amplification of telomeric repeats on the W sex chromosome in the sand lizard (Lacerta agilis). J Hered 106:753-757 (2015).
  21. Matsuda Y, Chapman VM: Application of fluorescence in situ hybridization in genome analysis of the mouse. Electrophoresis 16:261-272 (1995).
  22. Mayer W, Benyr G: Albumin-Evolution und Phylogenese in der Familie Lacertidae (Reptilia: Sauria). Ann Naturhist Mus Wien 96B:621-648 (1994).
  23. Meneveri R, Agresti A, Rocchi M, Marozzi A, Ginelli E: Analysis of GC-rich repetitive nucleotide sequences in great apes. J Mol Evol 40:405-412 (1995).
  24. Meyne J, Baker RJ, Hobart HH, Hsu TC, Ryder OA, et al: Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma 99:3-10 (1990).
  25. Miller OL Jr: The nucleolus, chromosomes, and visualization of genetic activity. J Cell Biol 91:15s-27s (1981).
  26. Nunn GB, Theisen BF, Christensen B, Arctander P: Simplicity-correlated size growth of the nuclear 28S ribosomal RNA D3 expansion segment in the crustacean order Isopoda. J Mol Evol 42:211-223 (1996).
  27. Odierna G, Olmo E, Cobror O: Taxonomic implications of NOR-localization in lacertid lizards. Amphibia-Reptilia 8:373-381 (1987).
  28. Odierna G, Kupriyanova LA, Capriglione T, Olmo E: Further data on sex chromosomes of Lacertidae and a hypothesis on their evolutionary trend. Amphibia-Reptilia 14:1-11 (1993).
  29. Odierna G, Aprea G, Arribas OJ, Capriglione T, Caputo V, Olmo E: The karyology of the Iberian rock lizards. Herpetologica 52:542-550 (1996).
  30. Olmo E, Signorino G: Chromorep: A reptiles chromosomes database (2005). http://chromorep.univpm.it (accessed October 26, 2016).
  31. Olmo E, Odierna G, Capriglione T: Evolution of sex-chromosomes in lacertid lizards. Chromosoma 96:33-38 (1987).
  32. Olmo E, Odierna G, Capriglione T, Cardone A: DNA and chromosome evolution in lacertid lizards, in Olmo E (ed): Cytogenetics of Amphibians and Reptiles (Advances in Life Sciences), pp 181-204 (Birkhäuser, Basel 1990).
  33. Olmo E, Odierna G, Capriglione T: The karyology of Mediterranean lacertid lizards, in Valakos ED, Boehme W, Pérez-Mellado V, Maragou P (eds): Lacertids of the Mediterranean Region: A Biological Approach, pp 61-84 (Hellenic Zoological Society, Athens 1993).
  34. Petrović V, Pérez-García C, Pasantes JJ, Šatovic E, Prats E, Plohl M: A GC-rich satellite DNA and karyology of the bivalve mollusk Donaxtrunculus: a dominance of GC-rich heterochromatin. Cytogenet Genome Res 124:63-71 (2009).
  35. Plohl M, Luchetti A, Meštrović N, Mantovani B: Satellite DNAs between selfishness and functionality: structure, genomics and evolution of tandem repeats in centromeric (hetero)chromatin. Gene 409:72-82 (2008).
  36. Pokorná M, Giovannotti M, Kratochvíl L, Kasai F, Trifonov VA, et al: Strong conservation of the bird Z chromosome in reptilian genomes is revealed by comparative painting despite 275 million years divergence. Chromosoma 120:455-468 (2011).
  37. Pokorná M, Rens W, Rovatsos M, Kratochvíl L: A ZZ/ZW sex chromosome system in the thick-tailed gecko (Underwoodisaurus milii; Squamata: Gekkota: Carphodactylidae), a member of the ancient gecko lineage. Cytogenet Genome Res 142:190-196 (2014).
  38. Rens W, Fu B, O'Brien PCM, Ferguson-Smith MA: Cross-species chromosome painting. Nat Protoc 1:783-790 (2006).
  39. Rojo V, Giovannotti M, Naveira H, Nisi Cerioni P, González-Tizón AM, et al: Karyological characterization of the endemic Iberian rock lizard, Iberolacerta monticola (Squamata, Lacertidae): insights into sex chromosome evolution. Cytogenet Genome Res 142:28-39 (2014).
  40. Rojo V, Martínez-Lage A, Giovannotti M, González-Tizón AM, Nisi Cerioni P, et al: Evolutionary dynamics of two satellite DNA families in rock lizards of the genus Iberolacerta (Squamata, Lacertidae): different histories but common traits. Chromosome Res 23:441-461 (2015).
  41. Rovatsos M, Kratochvíl L, Altmanová M, Johnson Pokorná M: Interstitial telomeric motifs in squamate reptiles: when the exceptions outnumber the rule. PLoS One 10:e0134985 (2015).
  42. Rovatsos M, Vukić J, Altmanová M, Johnson Pokorná M, Moravec J, Kratochvíl L: Conservation of sex chromosomes in lacertid lizards. Mol Ecol 25:3120-3126 (2016).
  43. Ruiz-Herrera A, Nergadze SG, Santagostino M, Giulotto E: Telomeric repeats far from the ends: mechanisms of origin and role in evolution. Cytogenet Genome Res 122:219-228 (2008).
  44. Sahar E, Latt SA: Energy transfer and binding competition between dyes used to enhance staining differentiation in metaphase chromosomes. Chromosoma 79:1-28 (1980).
  45. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, ed 2 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989).
  46. Schartl M, Schmid M, Nanda I: Dynamics of vertebrate sex chromosome evolution: from equal size to giants and dwarfs. Chromosoma 125: 553-571 (2016).
  47. Sindaco R, Jeremčenko V: The Reptiles of the Western Palearctic (Edizioni Belvedere, Latina 2008).
  48. Slimani T, Mateo Miras JA, Joger U, El Mouden EH, Geniez P, Martínez-Solano I: Acanthodactylus erythrurus. The IUCN Red List of Threatened Species. e.T61456A12470495 (2009).
  49. Srikulnath K, Matsubara K, Uno Y, Nishida C, Olsson M, Matsuda Y: Identification of the linkage group of the Z sex chromosomes of the sand lizard (Lacerta agilis, Lacertidae) and elucidation of karyotype evolution in lacertid lizards. Chromosoma 123:563-575 (2014).
  50. Sumner AT: A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304-306 (1972).
  51. Tamar K, Carranza S, Sindaco R, Moravec J, Trape J-F, Meiri S: Out of Africa: Phylogeny and biogeography of the widespread genus Acanthodactylus (Reptilia: Lacertidae). Mol Phylogenet Evol 103:6-18 (2016).
  52. Trifonov VA, Paoletti A, Caputo Barucchi V, Kalinina T, O'Brien PCM, et al: Comparative chromosome painting and NOR distribution suggest a complex hybrid origin of triploid Lepidodactylus lugubris (Gekkonidae). PLoS One 10:e0132380 (2015).
  53. Young MJ, O'Meally D, Sarre SD, Georges A, Ezaz T: Molecular cytogenetic map of the central bearded dragon, Pogona vitticeps (Squamata: Agamidae). Chromosome Res 21:361-374 (2013).

Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: August 28, 2017
Published online: November 29, 2017

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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