Developmental Neuroscience

Original Paper

NMR Spectroscopic Analysis of Rat Brain Development: In vitro Proton and Carbon Studies of Whole Tissue and Its Phospholipid Fraction

Zancanaro C. · Bolner A. · Righetti C.

Author affiliations

Magnetic Resonance Laboratory, DSMB, Section of Anatomy and Histology, University of Verona, Italy

Keywords: Folch extractionChromatographyUnsaturationPolyunsaturationC18 fatty acidsPerchloric acid extractionAgeing

Related Articles for ""
Dev Neurosci 2001;23:107–112
https://doi.org/10.1159/000048702

Log in to MyKarger to check if you already have access to this content.




Forgot your password
Sign up to MyKarger

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more
CHF 9.00 *
EUR 8.00 *
USD 9.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!


If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates

Select
* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: August 17, 2001
Issue release date: March – April

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 3

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

Abstract

The developmental patterns of the rat brain at several postnatal time points were investigated in Folch (chloroform-methanol) extracts. The chloroform- (lipid-containing) and water-soluble (cytosolic) fractions of whole-tissue extracts and the phospholipid fraction separated from the organic fraction by chromatography were analysed by means of high-resolution 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. Analysis of the cytosolic fraction showed the changing patterns of several brain metabolites during postnatal maturation, in full agreement with data obtained from perchloric acid extracts. 1H NMR spectroscopy of the phospholipid fraction allowed for quantitative evaluation of fatty acid acyl chain length, mean unsaturation and mean polyunsaturation. It was found that both mean unsaturation and polyunsaturation are lower in adult brain phospholipids than during the first 3 postnatal weeks. 13C NMR spectroscopy of the same fraction showed that the molar percentage of C18 fatty acids (oleic, linoleic, linolenic) in brain phospholipids is similar at all the investigated time points. These results indicate that the combination of Folch extraction with simple chromatographic procedures and NMR analysis yields useful data to define the chemical maturation of the brain.

© 2001 S. Karger AG, Basel



Related Articles:

References

  1. Alling C, Karlsson I (1973): Changes in lipid concentrations and fatty acid composition in rat cerebrum during maturation. J Neurochem 21:1051–1057.
  2. Bates TE, Williams SR, Gadian DG, Bell JD, Small RK; Iles RA (1989): 1H NMR study of cerebral development in the rat. NMR Biomed 2:225–229.
  3. Benjamins JA, McKhann GM (1972): Neurochemistry of development; in Albers WR, Siegel GJ, Katzman R, Agranoff BW (eds): Basic Neurochemistry. Boston, Little, Brown & Co, pp 269–298.
  4. Burri R, Bigler P, Straehl P, Posse S, Colombo J-P, Herschkowitz N (1990): Brain development: 1H magnetic resonance spectroscopy of rat brain extracts compared with chromatographic methods. Neurochem Res 15:1009–1016.
  5. Burri R, Lazeyras F, Aue WP, Strahel P, Bigler P, Althaus U, Herschkowitz N (1988): Correlation between 31P NMR phosphomonoester and biochemically determined phosporylethanolamine during development of the rat brain. Dev Neurosci 10:213–221.
  6. Chi Y, Gupta RK (1998): Alteration in membrane fatty acid unsaturation and chain length in hypertension as observed by 1H NMR spectroscopy. Am H Hypertens 11:340–348.
  7. Florian C-L, Williams SR, Bhakoo KK, Noble MD (1996): Regional and developmental variations in metabolite concentration in the rat brain and eye: A study using 1H NMR spectroscopy and high performance liquid chromatography. Neurochem Res 21:1065–1074.
  8. Folch J, Lees M, Sloane Stanley GH (1957): A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509.
  9. Kaluzny MA, Duncan LA, Mezzit MV, Epps DE (1985): Rapid separation of lipid classes in high yield and purity using bonded phase columns. J Lipid Res 26:135–140.
  10. Norton WT, Poduslo SE (1973): Myelination in rat brain: Changes in myelin composition during brain maturation. J Neurochem 21:759–773.
  11. O’Brien JS (1970): Lipids and myelination; in Himwich WA (ed): Developmental Neurobiology. Springfield, Thomas.
  12. Sinclair AJ, Crawford MA (1972): The incorporation of linolenic acid and docosahexaenoic acid into liver and brain lipids of developing rats. FEBS Lett 26:127–129.
  13. Wollemberg K (1991): Quantitative triacylglycerol analysis of whole vegetable seeds by 1H and 13C magic angle sample spinning NMR. J Am Oil Chem Soc 68:391–400.
  14. Yao FS, Caserta MT, Wyrwicz M (1999): In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57B1/6J) brain development. NMR Biomed 12:463–470.
  15. Zancanaro C (1994): Magnetic resonance spectroscopy investigations of brown adipose tissue and isolated brown adipocytes. J Lipid Res 35:2191–2199.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: August 17, 2001
Issue release date: March – April

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 3

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: https://www.karger.com/DNE

Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Article Tools
Article Details

2001, Vol.23, No. 2

March – April

Article

Recommend This
TOP