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Short Communication

Cellular Proliferation during Compensatory Renal Growth in Neonatal Rats Using Flow Cytometry

Han S.W. · Rha K.H. · Choi S.K. · Lee M.S.

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Department of Urology, Yonsei University College of Medicine, Seoul, Korea

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Nephron 2002;90:224–226

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Article / Publication Details

First-Page Preview
Abstract of Short Communication

Published online: January 30, 2002
Issue release date: February 2002

Number of Print Pages: 3
Number of Figures: 2
Number of Tables: 0

ISSN: 1660-8151 (Print)
eISSN: 2235-3186 (Online)

For additional information: https://www.karger.com/NEF

Abstract

Background: Compensatory renal growth consists of cellular enlargement and a small but consistent increase in DNA content. It has been assumed that the increase in the total renal DNA content was due to new cell formation. Methods: To test the hypothesis of whether cellular hyperplasia is the cause of the compensatory renal growth after the loss of the renal parenchyma and the timing of the DNA increase in neonatal rats, we performed cell cycle analysis using flow cytometry. Results: Following unilateral nephrectomy, the maximum increases of neonatal cortical cells entering the S phase occurred at 72 and 120 h (9.4 and 9.6% compared to 7.0 and 6.1% of the sham-operated group). Peak increases of neonatal kidney cortical cells entering the G2M phase occurred at 48 and 72 h (4.3 and 4.6% compared to 3.3 and 3.9% of the sham-operated group). Conclusion: DNA synthesis and replication occurs during compensatory renal growth following unilateral nephrectomy in neonatal rats as evidenced by an increase in cells entering both the S and G2M phases. In neonatal rats these events appear to be completed within 48–120 h after nephrectomy.

© 2002 S. Karger AG, Basel


References

  1. Johnson HA: Cytoplasmic response to overwork; in Nowinski WW, Gross RJ (eds): Compensatory Renal Hypertrophy. New York, Academic Press, 1969, pp 9–25.
  2. Skraastad O: Compensatory cell proliferation in the kidney after unilateral nephrectomy in mice. Virchows Arch 1987;53:97–101.
  3. Sands J, Dobbing J, Gratix CA: Cell number and cell size: Organ growth and development and the control of catch-up growth in rats. Lancet 1979;ii:503–505.
  4. Karp R, Brasel JA, Winick M: Compensatory kidney growth after uninephrectomy in adult and infant rats. J Dis Child 1971;121:186–189.
    External Resources
  5. Malt RA: Compensatory growth of the kidney. N Engl J Med 1996;280:1446–1449.
  6. Fleck C, Braulich H: Kidney function after unilateral nephrectomy. Exp Pathol 1984;25:3–8.
    External Resources
  7. Ring KS, Benson MC, Bandyk MG, Sawczuk IS: Detection of cellular proliferation during compensatory renal growth using flow cytometry. Nephron 1992;61:200–203.
  8. Ikeda M, Suzuki M, Miyazaki K, Takeuchi Y, Yamaguchi Y, Sakai S, Kawaguchi Y, Sakai O: Glomerular growth in the remnant kidney after a contralateral nephrectomy. Nephron 1993;65:95–99.
  9. Rollason HD: Compensatory hypertrophy of the kidney of the young rat with special emphasis on the role of cellular hyperplasia. Anat Rec 1949;104:263–285.

Article / Publication Details

First-Page Preview
Abstract of Short Communication

Published online: January 30, 2002
Issue release date: February 2002

Number of Print Pages: 3
Number of Figures: 2
Number of Tables: 0

ISSN: 1660-8151 (Print)
eISSN: 2235-3186 (Online)

For additional information: https://www.karger.com/NEF


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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