Caries Research

Research Article

Assessment of the Silver Penetration and Distribution in Carious Lesions of Deciduous Teeth Treated with Silver Diamine Fluoride

Li Y.a · Liu Y.a,b · Psoter W.J.c · Nguyen O.M.a · Bromage T.G.d · Walters M.A.e · Hu B.d · Rabieh S.d · Kumararaja F.C.a

Author affiliations

aDepartment of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY, USA
bDepartment of Cariology, Endodontology, and Operative Dentistry, Peking University School and Hospital of Stomatology, Beijing, China
cUniversity of Rochester Medical Center School of Medicine and Dentistry, Rochester, NY, USA
dDepartment of Biomaterials and Biomimetics, New York University College of Dentistry, New York, NY, USA
eDepartment of Chemistry, New York University College of Arts and Science, New York, NY, USA

Keywords: Early childhood cariesSilver diamine fluorideDeciduous toothEnamelDentin

Related Articles for ""
Caries Res 2019;53:431–440
https://doi.org/10.1159/000496210

Log in to MyKarger to check if you already have access to this content.




Forgot your password
Sign up to MyKarger

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more
CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!


If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates

Select
* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: July 10, 2018
Accepted: December 12, 2018
Published online: February 26, 2019
Issue release date: June 2019

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 0

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: https://www.karger.com/CRE

Abstract

The aim of this study was to determine the effects of 38% silver diamine fluoride (SDF) on carious lesions of human deciduous teeth. Ten extracted deciduous incisors with caries were collected and treated with SDF. After the treatment, the teeth were sectioned through the center of the carious lesion. The extent of sliver precipitation was examined using quantitative backscattered electron scanning electron microscopy (qBSE-SEM), energy-dispersive X-ray spectroscopy (EDX), and micro-computed tomography (micro-CT). The qBSE-SEM images revealed that the silver particles could penetrate through the pellicle complex, along with the rod sheaths into the demineralized enamel rods and the dentinal tubules, and form silver-enriched barriers surrounding the carious lesions at depths up to 2,490.2 μm (mean 744.7 ± 448.7 μm) within the dentinal tubules of the carious lesions, but less likely in the sound enamel. The EDX spectrum analysis revealed that carbon, oxygen, phosphorus, chlorine, silver, and calcium were the main elements detected in the lesions treated with SDF. Additionally, sodium, magnesium, aluminum, silicon, zinc, sulfur, and fluorine were detected as the minor elements within the SDF precipitation “zone.” The micro-CT analysis further showed that in the deep cavitated lesions, the silver precipitation could be observed in the pulp chamber. These findings provide new evidence defining the SDF mode of action for arresting caries and suggest that the application of a highly concentrated SDF solution on deciduous teeth should be used with caution for various carious lesions.

© 2019 S. Karger AG, Basel



Related Articles:

References

  1. Boyde A. Methodology of calcified tissue specimen preparation for scanning electron microscopy. In: Dickson GR, editor. Methods of Calcified Tissue Preparation. Amsterdam: Elsevier; 1984. p. 251–307.
  2. Boyde A, Davy KW, Jones SJ. Standards for mineral quantitation of human bone by analysis of backscattered electron images. Scanning. 1995;17 Suppl. V:6–7.
  3. Choi KY, Lee BS, Kim JH, Kim JJ, Jang Y, Choi JW, et al. Assessment of volatile sulfur compounds in adult and pediatric chronic tonsillitis patients receiving tonsillectomy. Clin Exp Otorhinolaryngol. 2018 Sep;11(3):210–5.
    External Resources
  4. Chu CH, Lo EC. Microhardness of dentine in primary teeth after topical fluoride applications. J Dent. 2008 Jun;36(6):387–91.
    External Resources
  5. Chu CH, Lo EC, Lin HC. Effectiveness of silver diamine fluoride and sodium fluoride varnish in arresting dentin caries in Chinese pre-school children. J Dent Res. 2002 Nov;81(11):767–70.
    External Resources
  6. De Menezes Oliveira MA, Torres CP, Gomes-Silva JM, Chinelatti MA, De Menezes FC, Palma-Dibb RG, et al. Microstructure and mineral composition of dental enamel of permanent and deciduous teeth. Microsc Res Tech. 2010 May;73(5):572–7.
    External Resources
  7. Fejerskov O. Pathology of dental caries. In: Fejerskov O, Nyvad B, Kidd EA, editors. Dental caries The disease and its clinical management. Oxford: John Wiley & Sons, Ltd.; 2015. p. 49–82.
  8. Fejerskov O, Larsen MJ. Demineralization and remineralization: the key to understanding clinical manifestations of dental caries. In: Fejerskov O, Nyvad B, Kidd EA, editors. Dental caries The disease and its clinical management. Oxford: John Wiley & Sons, Ltd.; 2015. p. 155–70.
  9. Gao SS, Zhang S, Mei ML, Lo EC, Chu CH. Caries remineralisation and arresting effect in children by professionally applied fluoride treatment – a systematic review. BMC Oral Health. 2016 Feb;16(1):12–21.
    External Resources
  10. Harris DC. Quantitative chemical analysis. 9th ed. New York (NY): W.H. Freeman and Co.; 2015.
  11. Horst JA, Ellenikiotis H, Milgrom PL. UCSF protocol for caries arrest using silver diamine fluoride: rationale, indications and consent. J Calif Dent Assoc. 2016 Jan;44(1):16–28.
    External Resources
  12. LeGeros RZ. Calcium phosphates in oral biology and medicine. Basel: Karger; 1991.
  13. Llodra JC, Rodriguez A, Ferrer B, Menardia V, Ramos T, Morato M. Efficacy of silver diamine fluoride for caries reduction in primary teeth and first permanent molars of schoolchildren: 36-month clinical trial. J Dent Res. 2005 Aug;84(8):721–4.
    External Resources
  14. Lo EC, Chu CH, Lin HC. A community-based caries control program for pre-school children using topical fluorides: 18-month results. J Dent Res. 2001 Dec;80(12):2071–4.
    External Resources
  15. Mei ML, Ito L, Zhang CF, Lo EC, Chu CH. Effect of laser irradiation on the fluoride uptake of silver diamine fluoride treated dentine. Lasers Med Sci. 2015 Apr;30(3):985–91.
    External Resources
  16. Mei ML, Lo ECM, Chu CH. Arresting dentine caries with silver diamine fluoride: what’s behind it? J Dent Res. 2018 Jul;97(7):751–8.
    External Resources
  17. Mei ML, Nudelman F, Marzec B, Walker JM, Lo EC, Walls AW, et al. Formation of Fluorohydroxyapatite with Silver Diamine Fluoride. J Dent Res. 2017 Sep;96(10):1122–8.
    External Resources
  18. Nanci A. Dentin-pulp complex. In: Ten Cate’s oral histology: development, structure, and function. St. Louis: Mosby; 2013a. p. 165–204.
  19. Nanci A. Enamel. In: Ten Cate’s oral histology: development, structure, and function. St. Louis: Mosby; 2013b. p. 122–64.
  20. Peng JJ, Botelho MG, Matinlinna JP. Silver compounds used in dentistry for caries management: a review. J Dent. 2012 Jul;40(7):531–41.
    External Resources
  21. Ren W, Xun Z, Wang Z, Zhang Q, Liu X, Zheng H, et al. Tongue coating and the salivary microbial communities vary in children with halitosis. Sci Rep. 2016 Apr;6(1):24481.
    External Resources
  22. Rosenblatt A, Stamford TC, Niederman R. Silver diamine fluoride: a caries “silver-fluoride bullet.” J Dent Res. 2009 Feb;88(2):116–25.
    External Resources
  23. Rossi G, Squassi A, Mandalunis P, Kaplan A. Effect of silver diamine fluoride (SDF) on the dentin-pulp complex: ex vivo histological analysis on human primary teeth and rat molars. Acta Odontol Latinoam. 2017 Apr;30(1):5–12.
    External Resources
  24. Rumble JR. Solubility product constants of inorganic salts. In: CRC handbook of chemistry and physics, ed 99 (Internet Version 2018). Boca Raton, FL: CRC Press/Taylor & Francis; 2018. Section 5 p. 1–8.
  25. Salako NO, Philip L. Comparison of the use of the Halimeter and the Oral Chroma™ in the assessment of the ability of common cultivable oral anaerobic bacteria to produce malodorous volatile sulfur compounds from cysteine and methionine. Med Princ Pract. 2011;20(1):75–9.
    External Resources
  26. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012 Jul;9(7):671–5.
    External Resources
  27. Shimooka S. On the penetration of silver nitrate and ammoniacal silver fluoride into microstructure of the sound dentin. Shigaku. 1972 Feb;59(6):534–66.
  28. Sinha N, Gupta A, Logani A, Shah N. Remineralizing efficacy of silver diamine fluoride and glass ionomer type VII for their proposed use as indirect pulp capping materials – Part II (A clinical study). J Conserv Dent. 2011 Jul;14(3):233–6.
    External Resources
  29. Suzuki T, Nishida M, Sobue S, Moriwaki Y. Effects of diammine silver fluoride on tooth enamel. J Osaka Univ Dent Sch. 1974 Sep;14:61–72.
    External Resources
  30. Takeshita T, Suzuki N, Nakano Y, Yasui M, Yoneda M, Shimazaki Y, et al. Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production. Sci Rep. 2012;2(1):215–22.
    External Resources
  31. Willershausen I, Schulte D, Azaripour A, Weyer V, Briseño B, Willershausen B. Penetration potential of a silver diamine fluoride solution on dentin surfaces. An ex vivo Study. Clin Lab. 2015;61(11):1695–701.
    External Resources
  32. Yamaga R, Nishino M, Yoshida S, Yokomizo I. Diammine silver fluoride and its clinical application. J Osaka Univ Dent Sch. 1972 Sep;12:1–20.
    External Resources
  33. Yee R, Holmgren C, Mulder J, Lama D, Walker D, van Palenstein Helderman W. Efficacy of silver diamine fluoride for Arresting Caries Treatment. J Dent Res. 2009 Jul;88(7):644–7.
    External Resources
  34. Zhi QH, Lo EC, Lin HC. Randomized clinical trial on effectiveness of silver diamine fluoride and glass ionomer in arresting dentine caries in preschool children. J Dent. 2012 Nov;40(11):962–7.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: July 10, 2018
Accepted: December 12, 2018
Published online: February 26, 2019
Issue release date: June 2019

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 0

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: https://www.karger.com/CRE

Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Article Tools
Article Details

2019, Vol.53, No. 4

June 2019

Article

Recommend This
TOP