Microbial Physiology
Research Article
The Life Cycle of Dictyostelium discoideum Is Accelerated via MAP Kinase Cascade by a Culture Extract Produced by a Synthetic Microbial ConsortiumKuwayama H.a · Higashinakagawa T.b,caGraduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
bInternational Center for Molecular, Cellular and Immunological Research, Tokyo Women’s Medical University, Tokyo, Japan cEM Research Organization, Okinawa, Japan |
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Article / Publication Details
Received: August 29, 2019
Accepted: October 27, 2019
Published online: December 05, 2019
Issue release date: July 2020
Number of Print Pages: 8
Number of Figures: 7
Number of Tables: 1
ISSN: 2673-1665 (Print)
eISSN: 2673-1673 (Online)
For additional information: https://www.karger.com/MIP
Abstract
A cellular slime mold, Dictyostelium discoideum, is an amoeboid organism that has a unique life cycle consisting of distinctly separated vegetative and developmental phases. Thus, this organism presents a rare opportunity in which to examine the effects of bioactive substances on separate cellular activities. In this research, we investigated the effect of a culture extract, termed EMXG, produced by a synthetic microbial consortium. EMXG promoted proliferative response of amoeba cells. It further accelerated the developmental phase, leading to the preferred fruiting body formation from fewer cells. Furthermore, EMXG modulated biological rhythm of this organism, that is, interval of oscillation of cAMP level observed in suspension starvation was significantly shortened. Concomitantly, the level of ERKB, a MAP kinase, was found to oscillate in a similar fashion to that of cAMP. Additionally, ErkB-deficient mutant amoeboid cells did not respond to proliferative stimulation by EMXG. These lines of evidence point to a likelihood that MAP kinase cascade is involved and further that ErkB could be the molecular target of EMXG.
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Article / Publication Details
Received: August 29, 2019
Accepted: October 27, 2019
Published online: December 05, 2019
Issue release date: July 2020
Number of Print Pages: 8
Number of Figures: 7
Number of Tables: 1
ISSN: 2673-1665 (Print)
eISSN: 2673-1673 (Online)
For additional information: https://www.karger.com/MIP
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