Cytogenetic and Genome Research

Original Article

Great Abundance of Satellite DNA in Proceratophrys (Anura, Odontophrynidae) Revealed by Genome Sequencing

da Silva M.J.a · Fogarin Destro R.a · Gazoni T.a · Narimatsu H.a · Pereira dos Santos P.S.a · Haddad C.F.B.b · Parise-Maltempi P.P.a

Author affiliations

aDepartamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Rio Claro, Brazil; bDepartamento de Zoologia e Centro de Aquicultura, Instituto de Biociências, Universidade Estadual Paulista - UNESP, Rio Claro, Brazil

Keywords: AmphibiaCytogeneticsFluorescence in situ hybridizationProceratophrys boieiRepeatExplorer

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Cytogenet Genome Res 2020;160:141-147
https://doi.org/10.1159/000506531

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: February 10, 2020
Published online: March 07, 2020
Issue release date: April 2020

Number of Print Pages: 7
Number of Figures: 2
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Most eukaryotic genomes contain substantial portions of repetitive DNA sequences. These are located primarily in highly compacted heterochromatin and, in many cases, are one of the most abundant components of the sex chromosomes. In this sense, the anuran Proceratophrys boiei represents an interesting model for analyses on repetitive sequences by means of cytogenetic techniques, since it has a karyotype with large blocks of heterochromatin and a ZZ/ZW sex chromosome system. The present study describes, for the first time, families of satellite DNA (satDNA) in the frog P. boiei. Its genome size was estimated at 1.6 Gb, of which 41% correspond to repetitive sequences, including satDNAs, rDNAs, transposable elements, and other elements characterized as non-repetitive. The satDNAs were mapped by FISH in the centromeric and pericentromeric regions of all chromosomes, suggesting a possible involvement of these sequences in centromere function. SatDNAs are also present in the W sex chromosome, occupying the entire heterochromatic area, indicating a probable contribution of this class of repetitive DNA to the differentiation of the sex chromosomes in this species. This study is a valuable contribution to the existing knowledge on repetitive sequences in amphibians. We show the presence of repetitive DNAs, especially satDNAs, in the genome of P. boiei that might be of relevance in genome organization and regulation, setting the stage for a deeper functional genome analysis of Proceratophrys.

© 2020 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: February 10, 2020
Published online: March 07, 2020
Issue release date: April 2020

Number of Print Pages: 7
Number of Figures: 2
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

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2020, Vol.160, No. 3

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