Nephron Experimental Nephrology

Original Paper

Increased Apoptosis in Acute Puromycin Aminonucleoside Nephrosis

Fernandez L.b · Romero M.a · Soto H.a · Mosquera J.a

Author affiliations

aInstituto de Investigaciones Clínicas ‘Dr Américo Negrette’ y bCentro de Cirugía Experimental, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela

Keywords: ApoptosisPuromycinNephrosisProliferationInfiltration

Related Articles for ""
Exp Nephrol 2001;9:99–108
https://doi.org/10.1159/000052600

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: January 11, 2001
Issue release date: March – April

Number of Print Pages: 10
Number of Figures: 7
Number of Tables: 2


eISSN: 1660-2129 (Online)

For additional information: https://www.karger.com/NEE

Abstract

Acute puromycin aminonucleoside nephrosis (PAN) in rats is characterized by heavy proteinuria associated with renal hypercellularity. The role of apoptosis in the resolution of renal hypercellularity was investigated in PAN. To study the participation of apoptosis in PAN, renal tissues were collected from nephrotic and control rats on weeks 1, 2 and 7 after a single puromycin aminonucleoside injection. Apoptosis was evaluated by light and electron microscopy. Apoptotic DNA fragmentation was detected by TUNEL staining. Renal tissues were also evaluated by the presence of leukocyte common antigen (LCA), ED1 (macrophages) and proliferating cell nuclear antigen (PCNA) with corresponding monoclonal antibodies. An increased number of apoptotic (TUNEL+) cells was observed in the glomerulus at week 1. Electron microscopy analysis showed glomerular apoptosis mainly in endothelial cells. In the interstitium and tubules, increased apoptosis was observed at weeks 1 and 2. Increased apoptosis was accompanied with increased LCA+, ED1+ and PCNA+ cells in the interstitium and with increased PCNA+ cells in tubules. There was a high significant correlation between the number of apoptotic cells and the number of interstitial LCA+, ED1+ and PCNA+ cells. Tubular PCNA expression was correlated with tubular apoptosis. We also observed significant correlation between glomerular, interstitial and tubular apoptosis with proteinuria during the nephrosis. Double staining analysis showed that about 13% of interstitial or tubular apoptotic cells were positive for PCNA. All these values returned to normal by week 7. These results indicate that apoptosis is involved in the repairing process of this disease model.

© 2001 S. Karger AG, Basel



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References

  1. Diamond JR, Pesek I: Glomerular tumor necrosis factor and interleukin-1 during acute aminonucleoside nephrosis. Lab Invest 1991;64:21–28.
    External Resources
  2. Diamond JR, Ding G, Frye J, Pesek-Diamond I: Glomerular macrophages and the mesangial proliferative response in the experimental nephrotic syndrome. Am J Pathol 1992;141:887–894.
    External Resources
  3. Eddy AA, Michael AF: Acute tubulointerstitial nephritis associated with aminonucleoside nephrosis. Kidney Int 1988;33:14–23.
    External Resources
  4. Jones CL, Buch S, Post M, McCulloch L, Liu E, Eddy AA: Renal extracellular matrix accumulation in acute puromycin aminonucleoside nephrosis in rats. Am J Pathol 1992;141:1381–1396.
    External Resources
  5. Mampaso FM, Egido J, Martinez-Quintero JC, Bricio T, Gonzalez E, Cobo ME, Pirotzky E, Braquet P, Hernando L: Interstitial mononuclear cell infiltrates in experimental nephrosis: Effect of PAF antagonist. Nephrol Dial Transplant 1989;4:1037–1044.
    External Resources
  6. Kerr JFR: Shrinkage necrosis. A distinct mode of cellular death. J Pathol 1971;105:13–20.
    External Resources
  7. Searle J, Kerr JFR, Bishop CJ: Necrosis and apoptosis: Distinct modes of cellular death with fundamentally different significance. Pathol Annu 1982;17:229–259.
    External Resources
  8. Wyllie AH, Kerr JFR, Currie AR: Cell death: The significance of apoptosis. Int Rev Cytol 1980;68:251–306.
    External Resources
  9. Bhathal PS, Gall JAM: Deletion of hyperplastic billiary epithelial cells by apoptosis following removal of the proliferative stimulus. Liver 1985;5:311–325.
    External Resources
  10. Bursch W, Lauer B, Timmermann-Trosiener I, Barthel G, Souppler J, Schulte-Hermann R: Controlled death (apoptosis) of normal and putative preneoplastic cells in rat liver following withdrawal of tumor promoters. Carcinogenesis 1984;5:453–458.
    External Resources
  11. Columbano A, Ledda-Columbano GM, Coni PP, Faa G, Liguori C, Santacruz GS, Pani P: Occurrence of cell death (apoptosis) during the involution of liver hyperplasia. Lab Invest 1985;52:670–675.
    External Resources
  12. Ledda-Columbano GM, Columbano A, Coni P, Faa G, Pani P: Cell detection by apoptosis during regression of renal hyperplasia. Am J Pathol 1989;135:657–662.
    External Resources
  13. Savill J: Apoptosis: A mechanism for regulation of the cell complement of inflamed glomeruli. Kidney Int 1992;41:607–612.
    External Resources
  14. Soto H, Mosquera J, Rodríguez-Iturbe B, Henriquez C, Pinto A: Apoptosis in proliferative glomerulonephritis: Decreased apoptosis expression in lupus nephritis. Nephrol Dial Transplant 1997;12:273–280.
    External Resources
  15. Takemura T, Murakami K, Miyazato H, Yagi K, Yoshioka K: Expression of Fas antigen and Bcl-2 in human glomerulonephritis. Kidney Int 1995;48:1886–1892.
    External Resources
  16. Shimizu A, Kitamura H, Masuda Y, Ishizaki M, Sugiga K, Yamanaka M: Apoptosis in the repair process of experimental proliferative glomerulonephritis. Kidney Int 1995;47:114–121.
    External Resources
  17. Baker AJ, Mooney A, Hughes J, Lombardi D, Johnson RJ, Savill J: Mesangial cells apoptosis: The major mechanism for resolution of glomerular hypercellularity in experimental mesangial proliferative nephritis. J Clin Invest 1994;94:2105–2116.
    External Resources
  18. Wijsman JH, Jonker RR, Keuzer R, Velde CJH, Cornelisse CJ, Dierendonck JH: New method to detect apoptosis in paraffin sections: In situ end-labeling of fragmented DNA. J Histochem Cytochem 1993;41:7–12.
    External Resources
  19. Gravrieli Y, Sherman Y, Ben-Sasson SA: Identification of programed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 1992;119:493–501.
    External Resources
  20. Nouwen EJ, Verstrepen WA, Buyssens N, Zhu MQ, De Broe ME: Hyperplasia, hypertrophy and phenotypic alterations in the distal nephron after acute proximal tubular injury in the rat. Lab Invest 1994;70:479–493.
    External Resources
  21. Eddy AA, McCulloch L, Liu E, Adams J: A relationship between proteinuria and acute interstitial disease in rats with experimental nephrosis syndrome. Am J Pathol 1991;138:1111–1123.
    External Resources
  22. Diamond JR, Anderson S: Irreversible tubulointerstitial damage associated with chronic aminonucleoside nephrosis. Amelioration by angiotensin I converting enzyme inhibition. Am J Pathol 1990;137:1323–1332.
    External Resources
  23. Arends MJ, Wyllie AH: Apoptosis: Mechanisms and role in pathology. Int Rev Exp Pathol 1991;32:223–254.
    External Resources
  24. Bravo R, MacDonald-Bravo H: Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: Association with DNA replication sites. J Cell Biol 1987;105:1549–1554.
    External Resources
  25. Savill J, Fadok V, Herison P, Haslett C: Phagocyte recognition of cells undergoing apoptosis. Immunol Today 1992;14:131–136.
  26. Eddy AA: Experimental insights into the tubulointerstitial disease accompanying primary glomerular lesions. J Am Soc Nephrol 1994;5:1273–1287.
    External Resources
  27. Rossmann P, Bukovcky A, Matousovic K, Holub M, Kral J: Puromycin aminonucleoside nephropathy: Ultrastructure, glomerular polyanion and cell surface markers. J Pathol 1986;148:337–348.
    External Resources
  28. Savill J, Mooney A, Hughes J: Apoptosis and renal scarring. Kidney Int 1996;49:S14–S17.
  29. Amore A, Cirina P, Iavarone A, Fiorucci GC, Coppo R: Proteinuria induces apoptosis in cultured tubular cells. J Am Soc Nephrol 1996;7:1691.
  30. Thomas GL, Yang B, Wagner BE, Savill J, Nahas ME: Cellular apoptosis and proliferation in experimental renal fibrosis. Nephrol Dial Transplant 1998;13:2216–2226.
    External Resources
  31. Coers W, Huitema S, van der Horst ML, Weening JJ: Puromycin aminonucleoside and Adriamycin disturb cytoskeletal and extracellular matrix protein organization, but not protein synthesis of cultured glomerular epithelial cells. Exp Nephrol 1994;2:40–50.
    External Resources
  32. Fishman JA, Karnovsky MJ: Effects of the aminonucleoside of puromycin on glomerular epithelial cells in vitro. Am J Pathol 1985;118:398–407.
    External Resources
  33. Shiiki H, Sasaki Y, Nishino T, Kimura T, Kirioka H, Fujimoto S, Dohi K: Cell proliferation and apoptosis of the glomerular epithelial cells in rats with puromycin aminonucleoside nephrosis. Pathology 1998;66:221–229.
  34. Davidoff AN, Mendelow BV: Puromycin-elicited c-myc mRNA superproduction precedes apoptosis in HL-60 leukemic cells. Anticancer Res 1993;13:2257–2260.
    External Resources
  35. Sugita M, Morita T, Yonesaki T: Puromycin induces apoptosis of developing chick sympathetic neurons in a similar manner to NGF deprivation. Zool Sci 1995;12:419–425.
    External Resources
  36. Natori Y, Igawa Y, Nakao N, Natori Y: Cytotoxicity of sera from rats with puromycin aminonucleoside nephrosis. Nephron 1996;73:258–263.
    External Resources
  37. Diamond JR, Karnovsky MJ: Focal and segmental glomerulosclerosis following a single intravenous dose of puromycin aminonucleoside. Am J Pathol 1986;122:481–487.
    External Resources
  38. Sugiyama H, Kashihara N, Yamasaki Y, Serikawa T, Okamoto K, Kanao K, Maeshima Y, Makino H, Ota Z: Reactive oxygen species induce apoptosis in cultured mesangial cells (abstract). J Am Soc Nephrol 1993;6:796.
  39. Olbricht CJ, Cannon JK, Tisher CC: Cathepsin B and L in nephron segment of the rats with puromycin aminonucleoside nephrosis. Kidney Int 1987;32:354–361.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: January 11, 2001
Issue release date: March – April

Number of Print Pages: 10
Number of Figures: 7
Number of Tables: 2


eISSN: 1660-2129 (Online)

For additional information: https://www.karger.com/NEE

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2001, Vol.9, No. 2

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