Intracellular Signaling Pathways in Human Eosinophil Activation: Role of a β2 Integrin, αMβ2Kato M.a · Kimura H.b · Tachibana A.a · Motegi Y.a · Tokuyama K.a · Kita H.c · Morikawa A.a
aDepartment of Pediatrics, Gunma University School of Medicine, bGunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, Japan; cDepartment of Immunology and Internal Medicine, Mayo Clinic and Foundation, Rochester, Minn., USA
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Cellular adhesion is crucial roles for eosinophil effector functions such as degranulation. Here, we focused on the role of a β2 integrin, αMβ2, in intracellular signaling pathways of human eosinophil activation. We found that the ligation of αMβ2 triggers the activation of an intracellular signaling cascade, including protein tyrosine phosphorylation and phosphoinositide turnover, and subsequent cellular degranulation in human eosinophils. These signaling events may play important roles in adhesion-dependent cellular functions of eosinophils.
Butterfield JH, Leiferman KM, Gleich GJ: Eosinophil-associated diseases; in Frank MM, Austen KF, Claman HN, Unanue ER (eds): Samuter’s Immunologic Diseases. Boston, Little, Brownand, 1995, pp 501–527.
- Butterworth AE, Remold HG, Houba V, David JR, Franks D, David PH, Sturrock RF: Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: Mediation by IgG, and inhibition by antigen-antibody complexes. J Immunol 1977;118:2230–2236.
- Springer TA: Adhesion receptors of the immune system. Nature 1990;346:425–434.
- Hartnell A, Moqbel R, Walsh GM, Bradley B, Kay AB: Fcγ and CD11/CD18 receptor expression on normal density and low density human eosinophils. Immunology 1990;69:264–270.
- Kaneko M, Horie S, Kato M, Gleich GJ, Kita H: A crucial role for β2 integrin in the activation of eosinophils stimulated by IgG. J Immunol 1995;155:2631–2641.
Horie S, Kita H: CD11b/CD18 (Mac-1) is required for degranulation of human eosinophils induced by human recombinant granulocyte-macrophage colony-stimulating factor and platelet-activating factor. J Immunol 1994;152:5427–5467.
- Laudanna C, Melotti P, Bonizzato C, Piacentini G, Boner A, Serra MC, Berton G: Ligation of members of the β1 or the β2 subfamilies of integrins by antibodies triggers eosinophil respiratory burst and spreading. Immunology 1993;80:273–280.
- Kato M, Abraham RT, Okada S, Kita H: Ligation of the β2 integrin triggers activation and degranulation of human eosinophils. Am J Respir Cell Mol Biol 1998;18:675–686.
- Kita H, Horie S, Gleich GJ: Extracellular matrix proteins attenuate activation and degranulation of stimulated eosinophils. J Immunol 1996;156:1174–1181.
- Kato M, Tokuyama K, Morikawa A, Kuroume T, Barnes PJ: Platelet-activating factor-induced enhancement of superoxide anion generation in guinea pigs. Eur J Pharmacol 1993;232:7–12.
- Nakamura M, Honda Z, Izumi T, Sakanaka C, Mutoh H, Minami M, Bito H, Sayama Y, Matsumoto T, Noma M, Simizu T: Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. J Biol Chem 1991;266:20400–20405.
- Lub M, van Kooyk Y, Fridor CG: Ins and outs of LFA-1. Immunol Today 1995;16:479–483.
- Canonica GW, Ciprandi G, Pesce GP, Buscaglia S, Paolieri F, Bagnasco M: ICAM-1 on epithelial cells in allergic subjects: A hallmark of allergic inflammation. Int Arch Allergy Immunol 1995;107:99–102.
Wegner CD, Gundel R, Churchill L, Letts LG: Adhesion glycoproteins as regulators of airway inflammation: Emphasis on the role of ICAM-1; in Holgate ST, Austen KF, Lichtenstein LM, Kay AB (eds): Asthma: Physiology, Immunopharmacology, and Treatment. London, Academic Press, 1993, pp 227–241.
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.