International Archives of Allergy and Immunology

Original Paper

Roles of CD4+ and CD8+ T Cells in Adjuvant Activity of Diesel Exhaust Particles in Mice

Fujimaki H.a · Ui N.b · Ushio H.a · Nohara K.a · Endo T.b

Author affiliations

aNational Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, and bJikei University School of Medicine, Nishishinbashi, Minato-ku, Tokyo, Japan

Keywords: AdjuvantDiesel exhaust particlesAllergyTh1/Th2

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Int Arch Allergy Immunol 2001;124:485–496
https://doi.org/10.1159/000053784

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: April 27, 2001
Issue release date: April 2001

Number of Print Pages: 12
Number of Figures: 7
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Through an imbalance in Th1 and Th2 cytokine profiles, diesel exhaust particles (DEP) are thought to induce Th2-dominated IgE and IgG1 production. However, the roles of CD4+ and CD8+ T-cell subtypes in the increased immune responses to antigen in mice exposed to DEP are unclear. In the present study, we investigated whether treatment with anti-CD4 or anti-CD8 mAb abrogated the adjuvant activity of DEP. On day –1 and day 1, each group of mice was injected intraperitoneally with anti-CD4, anti-CD8, or rat IgG (vehicle). On day 0, the mice were immunized with ovalbumin (OVA) or OVA plus DEP. After 3 weeks, each mouse was boosted with 10 µg of OVA alone. On day 7 after the first injection with OVA+DEP or OVA alone, the numbers of total, IA+, CD80+/IA+ and CD86+/IA+ cells in peritoneal exudate cells (PEC) were higher in OVA+DEP-immunized mice than in OVA-immunized mice. Depletion of CD8+ cells resulted in a modulation of the production of granulocyte-macrophage colony-stimulating factor, IL-12 and PGE2 in peritoneal exudate fluid from OVA+DEP-immunized mice. On day 28, DEP injection markedly increased IL-4 production in the culture supernatants of spleen cells from CD4+ or CD8+-depleted mice. Depletion of CD8+ cells in OVA+DEP-immunized mice resulted in a decrease in IFN-γ production compared with that in OVA-immunized mice. Adjuvant activity of DEP was observed in anti-OVA IgE, anti-OVA IgG1, anti-OVA IgG3, and total IgE production. Depletion of CD4+ T cells abrogated the adjuvant effect of DEP on anti-OVA IgE, and anti-OVA IgG1 production in plasma. However, depletion of CD8+ T cell inhibited the upregulated anti-OVA IgG3 production. These findings suggest that DEP injection may affect not only the function of CD4+ cells but also that of CD8+ T-cell subsets to modulate the synthesis of proinflammatory cytokine in PEC and type-1 and type-2 cytokine production in spleens.

© 2001 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: April 27, 2001
Issue release date: April 2001

Number of Print Pages: 12
Number of Figures: 7
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

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2001, Vol.124, No. 4

April 2001

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