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Gene Mapping, Cloning and Sequencing

Genomic organization of the human NFAT5 gene: exon-intron structure of the 14-kb transcript and CpG-island analysis of the promoter region

Dalski A. · Schwinger E. · Zühlke C.

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Institut für Humangenetik, Universität Lübeck, Lübeck (Germany)

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Cytogenet Cell Genet 93:239–241 (2001)

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Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning and Sequencing

Published online: August 23, 2001
Issue release date: 2001

Number of Print Pages: 3
Number of Figures: 4
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

NFAT5, also known as tonicity enhancer binding protein (TonEBP) or NFATL1, is a new member of the immunologically important NFAT protein family. Despite its obvious relationship to this transcription factor family, NFAT5 shows distinct ways of regulation and function. The complete coding sequence and its alternative splice forms have been described previously. This sequence only refers to less than half of the total mRNA length. High conservation of this gene was shown among man, mouse, and pig. Here we report the cloning of the complete 14-kb cDNA sequence, its genomic organization, and a possible fourth isoform of the corresponding protein. Additionally, we describe the promoter region by CpG-island methylation analysis.   

© 2001 S. Karger AG, Basel


References

  1. Dalski A, Wagner H-J, Schwinger E, Zühlke Ch: Quantitative PCR analysis of different splice forms of NFAT5 revealed specific gene expression in fetal and adult brain. Mol Brain Res 83:125–127 (2000).
    External Resources
  2. Frohman MA: Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE. Meth Enzym 218:340–356 (1993).
  3. Hebinck A, Dalski A, Engel H, Mattei MG, Hawken R, Schwinger E, Zühlke C: Assignment of transcription factor NFAT5 to human chromosome 16q22.1, murine chromosome 8D and porcine chromosome 6p1.4 and comparison of the polyglutamine domains. Cytogenet Cell Genet 90:68–70 (2000).
  4. López-Rodríguez C, Aramburu J, Rakeman AS, Copeland NG, Gilbert DJ, Thomas S, Disteche C, Jenkins NA, Rao A: NFAT5: The NF-AT family of transcription factors expands in a new direction. Cold Spring Harbor Symp 64:517–526 (1999).
    External Resources
  5. Miyakawa H, Woo SK, Dahl SC, Handler JS, Kwon HM: Tonicity-responsive enhancer binding protein, a Rel-like protein that stimulates transcription in response to hypertonicity. Proc natl Acad Sci, USA 96:2538–2542 (1999).
  6. Serfling E, Berberich-Siebelt F, Chucpilo S, Jankevics E, Klein-Hessling S, Twardzik T, Avots A: The role of NF-AT transcription factors in T cell activation and differentiation. Biochem biophys Acta 1498:1–18 (2000).
  7. Trama J, Lu Q, Hawley RG, Ho SN: The NFAT-related protein NFATL1 (TonEBP/NFAT5) is induced upon T cell activation in a calcineurin-dependent manner. J Immun 165:4884–4894 (2000).

Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning and Sequencing

Published online: August 23, 2001
Issue release date: 2001

Number of Print Pages: 3
Number of Figures: 4
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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