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Melatonin Suppresses Reactive Oxygen Species in UV-Irradiated Leukocytes More than Vitamin C and Trolox

Fischer T.W. · Scholz G. · Knöll B. · Hipler U.-C. · Elsner P.

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Department of Dermatology and Allergology, Friedrich-Schiller University Jena, Jena, Germany

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Skin Pharmacol Appl Skin Physiol 2002;15:367–373

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: September 16, 2002
Issue release date: September – October

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: https://www.karger.com/SPP

Abstract

To prove the relative potency of melatonin as a radical scavenger in UV-irradiated leukocytes, it was compared to other antioxidative substances such as trolox and vitamin C. Human leukocytes were isolated from EDTA whole blood and incubated with melatonin, trolox and vitamin C. The experiments were performed in a wide concentration range from 0.1 nM to 1 mM and in a small concentration range from 0.5 to 2 mM (mel), 5 mM (trolox) and 10 mM (vit. C). Irradiation was performed with UV-light (280-360 nm) at a dose of 750 mJ/cm2. Radical formation was measured by the chemiluminescence technique. The maximum effect of radical suppression was seen at a concentration of 10 nM (p = 0.003) and 1 mM melatonin (p < 0.001) and vitamin C (p = 0.002; p < 0.001), respectively. ROS suppression by trolox was only significant at 1 mM (p < 0.001). In the small concentration range, a linear dose-response relationship was found and melatonin showed the strongest radical suppression (IC50 = 0.21 mM) followed by vitamin C (IC50 = 0.26 mM) and trolox (IC50 = 1.03 mM).

© 2002 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: September 16, 2002
Issue release date: September – October

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: https://www.karger.com/SPP


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