Ophthalmologica

Original Paper · Travail original · Originalarbeit

RPE in Perfusion Tissue Culture and Its Response to Laser Application

Preliminary Report

Framme C. · Kobuch K. · Eckert E. · Monzer J. · Roider J.

Author affiliations

University Eye Hospital Regensburg, Germany

Keywords: EyeExperimentalPathologyRetinal pigment epitheliumPerfusion tissue cultureLaser photocoagulationCell response

Related Articles for ""
Ophthalmologica 2002;216:320–328
https://doi.org/10.1159/000066184

Log in to MyKarger to check if you already have access to this content.




Forgot your password
Sign up to MyKarger

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more
CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!


If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates

Select
* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Original Paper · Travail original · Originalarbeit

Received: June 29, 2001
Accepted: February 22, 2002
Published online: November 08, 2002
Issue release date: September – October

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 1

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: https://www.karger.com/OPH

Abstract

Purpose: To study the effects of conventional laser application on the retinal pigment epithelium (RPE) in a perfusion tissue culture model of porcine retinal pigment epithelium without overlying neurosensory retina. Methods: RPE with underlying choroid was prepared from enucleated porcine eyes and fixed in a holding ring (Minusheet®). Specimens were then placed in two-compartment tissue culture containers (MinuCell & Minutissue, Bad Abbach, Germany) and were cultured during continuous perfusion with culture medium at both sides of the entire specimen, the upper RPE and the lower choroid (12 specimens out of 6 eyes). Cultures were kept for 1, 3, 7 and 14 days and were examined histologically. Laser treatment was performed on each tissue ring by application of 3 × 3 laser burns one day after culture began (argon ion laser, wavelength: 514 nm, pulse duration: 100 ms; spot size: 200 µm) using different energy levels (400–1,000 mW); (16 specimens out of 8 eyes). Results: During laser treatment a marked lightening of the RPE with centrifugal spreading was observed. Using higher levels of energy, a contraction of the RPE towards the center of the laser spot was noticed. One day after laser photocoagulation histology revealed destruction of RPE; within 3–7 days of culture, migration and proliferation of neighboring cells was observed in several lesions. After 7 days the initial defect of the irradiated area was covered with dome shaped RPE cells and after 14 days multilayered RPE cells were showing ongoing proliferation. However, there were also cases without proliferation after laser treatment. The non-treated, continuously perfused RPE showed regular appearance in histological sections: during the first 7 days of culture, light microscopy revealed a normal matrix with a well-differentiated RPE monolayer. Subsequently proliferation even without treatment was observed and after 14 days the RPE became multilayered. Conclusion: It was possible to study the early healing response to the effect of laser treatment using the permanently perfused tissue culture system. A marked proliferation and repair of the laser defect could be observed in several but not all lesions. After 14 days even without laser treatment a proliferative multilayered RPE was present. Although this limits the use of the system for longer than 7 days, it seems to be useful for investigation of RPE-related disorders.

© 2002 S. Karger AG, Basel



Related Articles:

References

  1. Glaser BM, Campochiaro PA, Davis JL, Jerdan JA: Retinal pigment epithelium cells release inhibitors of neovascularization. Ophthalmology 1987;94:780–784.
    External Resources
  2. Marshall J: Interactions between sensory cells, glia cells and the retinal pigment epithelium and their response to photocoagulation. Dev Ophthalmol 1981;2:308–317.
    External Resources
  3. Pollack A, Korte GE: Repair of retinal pigment epithelium and its relationship with capillary endothelium after krypton photocoagulation. Invest Ophthalmol Vis Sci 1990;31:890–898.
    External Resources
  4. Tso MO, Cunha-Vaz JG, Shih C, Jones CW: Clinicopathologic study of blood retinal barrier in experimental diabetes mellitus. Arch Ophthalmol 1980;98:2032–2040.
    External Resources
  5. Wallow I: Long-term changes in photocoagulation burns. Dev Ophthalmol 1981;2:318–327.
    External Resources
  6. Wallow IH, Tso MO: Repair after xenon arc photocoagulation. II. A clinical and light microscopic study of the evolution of retinal lesions in the rhesus monkey. Am J Ophthalmol 1973;75:610–626.
    External Resources
  7. Wallow IH: Repair of the pigment epithelial barrier following photocoagulation. Arch Ophthalmol 1984;102:126–135.
    External Resources
  8. Wolbarsht MT, Landers MB: The rationale of photocoagulation therapy for proliferative diabetic retinopathy: A review and model. Ophthalmic Surg 1980;11:235–245.
    External Resources
  9. Gabel VP, Birngruber R, Hillenkamp F: Visible and near infrared light absorption in pigment epithelium and choroid; in Shimizu K (ed): International Congress Series No.450, XXIII Concilium Ophthalmologicum, Kyoto. Princeton, Excerpta Medica, 1978, pp 658–662.
  10. Goldhar SW, Basu PK, Ranadive NS: Phagocytosis by retinal pigment epithelium: Evaluation of modulating agents with an organ culture model. Can J Ophthalmol 1984;19:33–35.
    External Resources
  11. Rosenstock T, Basu R, Basu PK, et al: Quantitative assay of phacocytosis by retinal pigment epithelium: An organ culture model. Exp Eye Res 1980;30:719–729.
    External Resources
  12. Tsuboi S, Pederson JE, Toris CB: Functional recovery of retinal pigment epithelial damage in experimental retinal detachment. Invest Ophthalmol Vis Sci 1987;28:1788–1794.
    External Resources
  13. Hay ED: Collagen and embryonic development; in Hay ED (ed): Cell Biology of Extracellular Matrix. New York, Plenum Press, 1981, pp 379–409.
  14. Reh TA, Nagy T, Gretton H: Retinal pigmented epithelial cells induced to transdifferentiate to neurons by laminin. Nature 1987;330:68–71.
    External Resources
  15. Whittaker JR: Translational competition as a possible basis of modulation in retinal pigment cell cultures. J Exp Zool 1968;169:143–159.
    External Resources
  16. Del Priore LV, Glaser BM, Quigley HA, Dorman ME, Green R: Morphology of pig retinal pigment epithelium maintained in organ culture. Arch Ophthalmol 1988;106:1286–1290.
    External Resources
  17. Del Priore LV, Glaser BM, Quigley HA, Green R: Response of pig retinal pigment epithelium to laser photocoagulation in organ culture. Arch Ophthalmol 1989;107:119–122.
    External Resources
  18. Herrmann WA, Kobuch K, Kloth S, Framme C, Roider J: Full-thickness adult retina in perfusion culture. A new organotypical model for toxicity tests. Invest Ophthalmol Vis Sci 1999;40(suppl):S989.
  19. Kobuch K, Kloth S, Herrmann W, Eckert E, Roider J, Gabel VP: Adulte Netzhaut-Pigmentepithel-Präparate in der Perfusionskultur – Ein neues organtypisches in-vitro-Modell für physiologische und pharmako-toxikologische Studien. Ophthalmologe 1999;96(suppl 1):64.
  20. Minuth WW, Kloth S, Aigner J, Sittinger M, Rockl W: Organo-typical environment for cultured cells and tissues. Bioforum 1994;17:412–416.
  21. Minuth WW: US-patent No.5, 190, 878 – Apparatus for cultivating cells (1993).
  22. Barr-Nea L, Barishak YR: Behaviour of retinal pigment epithelium in organ culture conditions. Ophthalmic Res 1972/1973;4:321–327.
  23. Feeney L, Mixon RN: An in vitro model of phagocytosis in bovine and human retinal pigment epithelium: An organ culture model. Exp Eye Res 1980;30:719–729.
    External Resources
  24. Tso MOM, Albert D, Zimmerman LE: Organ culture of human retinal pigment epithelium and choroid: A model for the study of cytologic behaviour of RPE in vitro. Invest Ophthalmol Vis Sci 1973;12:554–566.
  25. Smiddy WE, Fine SL, Quigley HA, et al: Cell proliferation after laser photocoagulation in primate retina: An autoradiographic study. Arch Ophthalmol 1986;104:1065–1069.
    External Resources
  26. Smiddy WE, Fine SL, Quigley HA, et al: Comparison of krypton and argon laser photocoagulation: Results of simulated clinical treatment of primate retina. Arch Ophthalmol 1984;102:1086–1092.
    External Resources
  27. Smiddy WE, Fine SL, Quigley HA, et al: Simulated treatment of recurrent choroidal neovascularization in primate retina: Comparative histopathologic findings. Arch Ophthalmol 1985;103:428–433.
    External Resources
  28. Marshall J, Bird AC: A comparative histopathologic study of argon and krypton laser irradiations of the human retina. Br J Ophthalmol 1979;63:657–668.
    External Resources
  29. Marshall J, Clover G, Rothery S: Some new findings on retinal irradiation by krypton and argon lasers. Doc Ophthalmol 1984;36:21–37.
  30. Matsumoto M, Yoshimura N, Honda Y: Increased production of transforming growth factor-b2 from cultures human retinal pigment epithelial cells by photocoagulation. Invest Ophthalmol Vis Sci 1994;35:4245–4252.
    External Resources
  31. Pollack A, Heriot WJ, Henkind P: Cellular processes causing defects in Bruch’s membrane following krypton laser photocoagulation. Ophthalmology 1986;93:1113–1119.
    External Resources
  32. Pollack A, Korte GE: Repair of retinal pigment epithelium and choriocapillaries after laser photocoagulation: Correlations between scanning electron transmission and light microscopy. Ophthalmic Res 1997;29:393–404.
    External Resources
  33. Roider J, Michaud NA, Flotte TJ, Birngruber R: Response of the retinal pigment epithelium to selective photocoagulation. Arch Ophthalmol 1992;110:1786–1792.
    External Resources
  34. Campochiaro PA, Hackett SF: Corneal endothelial cell matrix promotes expression of differentiated features of retinal pigmented epithelial cells: Implication of laminin and basic fibroblast growth factor as active components. Exp Eye Res 1993;57:539–547.
    External Resources
  35. Martini B, Pandey R, Ogden TE, Ryan SJ: Cultures of human retinal pigment epithelium. Invest Ophthalmol Vis Sci 1992;33:516–521.
    External Resources
  36. Ozaki S, Kita M, Yamana T, Negi A, Honda Y: Influence of the sensory retina on healing of the rabbit retinal pigment epithelium. Curr Eye Res 1997;16:349–358.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Original Paper · Travail original · Originalarbeit

Received: June 29, 2001
Accepted: February 22, 2002
Published online: November 08, 2002
Issue release date: September – October

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 1

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: https://www.karger.com/OPH

Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Article Tools
Article Details

2002, Vol.216, No. 5

September – October

Article

Recommend This
Additional Information

Presented in part on: ARVO Annual Meeting, May 9–14, 1999, Fort Lauderdale, Florida, USA, and Annual Meeting of the German Ophthalmologic Society, DOG, September 23–26, 1999, Berlin, Germany.
TOP