Dose-Dependent and Preterm- Accentuated Diaplacental Transport of Nutritive Allergens in vitroEdelbauer M.a · Loibichler C.a · Witt A.b · Gerstmayr M.a · Putschögl B.a · Urbanek R.a · Szépfalusi Z.a
Departments of aPediatrics and bObstetrics and Gynecology, University of Vienna, Austria
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Article / Publication Details
Objective: The mechanisms by which nutritive allergens are transported from mother to fetus and the ensuing immunological response are incompletely understood. We investigated the role of different allergen concentrations in influencing the diaplacental allergen transport in preterm and term placentas. Method: Twenty-seven human term placentas and 12 preterm placentas were dually perfused in vitrofor up to 4 h by adding alternately two different nutritive allergens, β-lactoglobulin (BLG) or ovalbumin (OVA), at four different allergen concentrations (0.02, 0.2, 2 and 20 mg/ml) to the maternal perfusate medium. Allergen concentrations in fetal venous outflow samples collected during perfusion were measured by using specific ELISAs. Results: Perfusion of increasing allergen concentrations via the maternal circulation resulted in a concentration-dependent increase of fetal allergen uptake in all term and preterm placentas. A mean maternal-to-fetal ratio of 20,000/1 and 3,000/1 for BLG, and 40,000/1 and 5,000/1 for OVA was found in term and preterm placentas, respectively. Preterm placentas (27–36 weeks of gestation) were found to favor the diaplacental passage of nutritive allergens compared with placentas at term (>36 weeks of gestation). Conclusion: Maternal-to-fetal allergen transport occurs in a dose-dependent and molecular weight-dependent manner with clear accentuation in preterm placentas.
© 2003 S. Karger AG, Basel
Holt PG, O’Keeffe P, Holt BJ, Upham JW, Baron-Hay MJ, Suphioglu C, Knox B, Stewart GA, Thomas WR, Sly PD: T-cell ‘priming’ against environmental allergens in human neonates: Sequential deletion of food antigen reactivity during infancy with concomitant expansion of responses to ubiquitous inhalant allergens. Pediatr Allergy Immunol 1995;6/2:85–90.
Jones AC, Miles EA, Warner JO, Colwell BM, Bryant TN, Warner JA: Fetal peripheral blood mononuclear cell proliferative responses to mitogenic and allergenic stimuli during gestation. Pediatr Allergy Immunol 1996;7/3:109–116.
- Piastra M, Stabile A, Fioravanti G, Castagnola M, Pani G, Ria F: Cord blood mononuclear cell responsiveness to beta-lactoglobulin: T-cell activity in ‘atopy-prone’ and ‘non-atopy-prone’ newborns. Int Arch Allergy Immunol 1994;104:358–365.
- Piccinni MP, Mecacci F, Sampognaro S, Manetti R, Parronchi P, Maggi E, Romagnani S: Aeroallergen sensitization can occur during fetal life. Int Arch Allergy Immunol 1993;102:301–303.
- Szépfalusi Z, Loibichler C, Pichler J, Reisenberger K, Ebner C, Urbanek R: Direct evidence for transplacental allergen transfer. Pediatr Res 2000;48:404–407.
- Holloway JA, Warner JO, Vance GH, Diaper ND, Warner JA, Jones CA: Detection of house-dust-mite allergen in amniotic fluid and umbilical cord blood. Lancet 2000;356:1900–1902.
- Lin H, Mosmann TR, Guilbert L, Tuntipopipat S, Wegmann TG: Synthesis of T helper 2-type cytokines at the maternal-fetal interface. J Immunol 1993;151:4562–4573.
- Wegmann TG, Lin H, Guilbert L, Mosmann TR: Bidirectional cytokine interactions in the maternal-fetal relationship: Is successful pregnancy a TH2 phenomenon? Immunol Today 1993;14:353–356.
- Jones CA, Kilburn SA, Warner JA, Warner JO: Intrauterine environment and fetal allergic sensitization. Clin Exp Allergy 1998;28:655–659.
- Constant S, Pfeiffer C, Woodard A, Pasqualini T, Bottomly K: Extent of T cell receptor ligation can determine the functional differentiation of naive CD4+ T cells. J Exp Med 1995;182:1591–1596.
- Hosken NA, Shibuya K, Heath AW, Murphy KM, O’Garra A: The effect of antigen dose on CD4+ T helper cell phenotype development in a T cell receptor-αβ-transgenic model. J Exp Med 1995;182:1579–1584.
Hemmings DG, Lowen B, Sherburne R, Sawicki G, Guilbert LJ: Villous trophoblasts cultured on semi-permeable membranes form an effective barrier to the passage of high and low molecular weight particles. Placenta 2001;22/1:70–79.
- Schneider H, Huch A: Dual in vitro perfusion of an isolated lobe of human placenta: Method and instrumentation. Contrib Gynecol Obstet 1985;13:40–47.
- Panigel M: Past, present, and future of placental perfusion experiments. Contrib Gynecol Obstet 1985;13:132–136.
- Miller RK, Wier PJ, Maulik D, di Sant’Agnese PA: Human placenta in vitro: Characterization during 12 h of dual perfusion. Contrib Gynecol Obstet 1985;13:77–84.
Challier JC, Schneider H, Dancis J: In vitro perfusion of human placenta. V. Oxygen consumption. Am J Obstet Gynecol 1976;126/2:261–265.
Malek A, Sager R, Schneider H: Transport of proteins across the human placenta. Am J Reprod Immunol 1998;40:347–351.
- Leach L, Firth JA: Fine structure of the paracellular junctions of terminal villous capillaries in the perfused human placenta. Cell Tissue Res 1992;268:447–452.
Edwards D, Jones CJ, Sibley CP, Nelson DM: Paracellular permeability pathways in the human placenta: A quantitative and morphological study of maternal-fetal transfer of horseradish peroxidase. Placenta 1993;14/1:63–73.
- Brownbill P, Edwards D, Jones C, Mahendran D, Owen D, Sibley C, Johnson R, Swanson P, Nelson DM: Mechanisms of alphafetoprotein transfer in the perfused human placental cotyledon from uncomplicated pregnancy. J Clin Invest 1995;96:2220–2226.
- Leichtweiss HP, Carstensen M, Schröder H, Rachor D: Some physiological properties of the isolated human placenta. Contrib Gynecol Obstet 1985;13:70–76.
- Elliott BD, Schenker S, Langer O, Johnson R, Prihoda T: Comparative placental transport of oral hypoglycemic agents in humans: A model of human placental drug transfer. Am J Obstet Gynecol 1994;171:653–660.
- Paganelli R, Levinsky RJ: Solide phase radioimmunoassay for detection of circulating food protein antigens in human serum. J Immunol Methods 1980;37:333–341.
Husby S, Jesenius JC, Svehag SE: Passage of undegraded dietary antigen into the blood of healthy adults: Further characterization of the kinetics of uptake and the size distribution of the antigen. Scand J Immunol 1986;23:447–455.
Malek A, Sager R, Lang AB, Schneider H: Protein transport across the in vitro perfused human placenta. Am J Reprod Immunol 1997;38:263–271.
- Prescott SL, Holt PG, Jenmalm M, Bjorksten B: Effects of maternal allergen-specific IgG in cord blood on early postnatal development of allergen-specific T-cell immunity. Allergy 2000;55:470–475.
Firan M, Bawdon R, Radu C, Ober RJ, Eaken D, Antohe F, Ghetie V, Wards ES: The MHC class I-related receptor, FcRn, plays an essential role in the maternofetal transfer of γ-globulin in humans. Int Immunol 2001;8:993–1002.
- Berryman M, Rodewald R: β2-Microglobulin co-distributes with the heavy chain of the intestinal IgG-Fc receptor through the transepithelial transport pathway of the neonatal rat. J Cell Sci 1995;108:2347–2360.
- Dickinson BL, Badizadegan K, Wu Z, Ahouse JC, Zhu X, Simister NE, Blumberg RS, Lencer WI: Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line. J Clin Invest 1999;104:903–911.
Pathirana C, Goulding NJ, Gibney MJ, Pitts JM, Gallagher PJ, Taylor TG: Immune tolerance produced by pre- and postnatal exposure to dietary antigens. Int Arch Allergy Appl Immunol 1981;66/1:114–118.
Dahl GM, Telemo E, Westrom BR, Jakobsson I, Lindberg T, Karlsson BW: The passage of orally fed proteins from mother to foetus in the rat. Comp Biochem Physiol A 1984;77/2:199–201.
- Gammon G, Dunn K, Shastri N, Oki A, Wilbur S, Sercarz EE: Neonatal T-cell tolerance to minimal immunogenic peptides is caused by clonal inactivation. Nature 1986;319:413–415.
- Ridge JP, Fuchs EJ, Matzinger P: Neonatal tolerance revisited: Turning on newborn T cells with dendritic cells. Science 1996;271:1723–1726.
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