Chemotherapy
Experimental Chemotherapy
Ionic Binding of 3H-Gentamicin and Short-Time Bactericidal Activity of Gentamicin against Pseudomonas aeruginosa Isolates with Different Lipopolysaccharide StructuresSaika T. · Hasegawa M. · Kobayashi I. · Nishida M.Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo, Japan
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Article / Publication Details
Published online: June 25, 1999
Issue release date: July – August
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 1
ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)
For additional information: https://www.karger.com/CHE
Abstract
The clinical isolates of Pseudomonas aeruginosa can be roughly classified into long- and short-LPS strains and LPS-deficient strains. Ionic binding of 3H-gentamicin was the highest in the long-LPS strains followed in descending order by short-LPS and LPS-deficient strains. However, PAC605 strain, completely lacking in the repeated units of O-polysaccharide and also lacking in some of the neutral sugar residues of the core oligosaccharide region, highly bound to 3H-gentamicin and it is suggested that the negatively charged sites on the deep-core oligosaccharide region and/or on lipid A participated in the binding to 3H-gentamicin. This manner of binding may be also applied to P. aeruginosa No. 45 (LPS-deficient), a clinical isolate.
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References
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Article / Publication Details
Published online: June 25, 1999
Issue release date: July – August
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 1
ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)
For additional information: https://www.karger.com/CHE
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