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Original Paper

Expression of RhoC Is Associated with Metastasis of Gastric Carcinomas

Kondo T.a · Sentani K.a · Oue N.a · Yoshida K.b · Nakayama H.a · Yasui W.a

Author affiliations

aDepartment of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, and bDepartment of Surgical Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan

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Pathobiology 2004;71:19–25

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: January 17, 2003
Accepted: March 07, 2003
Published online: October 16, 2003
Issue release date: October 2003

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 1

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: https://www.karger.com/PAT

Abstract

Objectives:RhoC, a member of the ras-related small GTPase protein family, regulates cytoskeletal structures and has the potential to transform cultured cells. It has recently been reported that RhoC contributes to the metastatic phenotype of melanoma cells. The purpose of this study was to clarify its biological relevance to gastric carcinogenesis and metastasis. Methods: We examined the expression of RhoC by quantitative RT-PCR in 51 cases of gastric carcinoma tissues from prior surgical cases (intestinal type: 24 cases, diffuse type: 27 cases) and in 8 gastric carcinoma cell lines. Results:RhoC expression levels in primary tumors were significantly higher in cases with metastasis than in those without metastasis (p = 0.0202; Mann-Whitney U test). RhoC expression levels in primary tumor and their metastatic tumors were significantly higher than their corresponding nonneoplastic mucosa (p = 0.0357, and 0.0173, respectively; Wilcoxon signed rank test). RhoC mRNA expression was confirmed in the gastric carcinoma cell lines. Conclusion: Our findings suggest that elevated expression of the RhoC gene may be involved in the metastasis of gastric carcinomas and may be a good genetic marker for the prediction of a metastatic potential.

© 2004 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: January 17, 2003
Accepted: March 07, 2003
Published online: October 16, 2003
Issue release date: October 2003

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 1

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: https://www.karger.com/PAT


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