Analysis of Epidermal Lipids of the Healthy Human Skin: Factors Affecting the Design of a Control PopulationDe Paepe K.a · Weerheim A.c · Houben E.a · Roseeuw D.b · Ponec M.c · Rogiers V.a
Departments of aToxicology and Dermato-Cosmetology and bDermatology, Vrije Universiteit Brussel, Brussels, Belgium; cSkin Research Laboratory, Leiden University Medical Center, Leiden, The Netherlands
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Article / Publication Details
The intervariability of studies on the lipids of human epidermis and stratum corneum is high because of the different origin of the skin samples and the variety of extraction methods used. In the present work, a high-performance thin-layer chromatographic technique has been used to study the parameters age, sex, and anatomical site for their effects on the lipid profiles recovered from healthy epidermal skin biopsy specimens. It was found that sex-related differences were seen at the level of the total ceramide concentration. Observed decreases in lipid concentration, due to ageing, depended on the anatomical site. Therefore, these variables should be controlled in a reproducible and standardized way in order to be able to study the direct relationship between skin condition and barrier lipid composition. Only when this relation is established, results of topical treatment can be scientifically evaluated.
© 2004 S. Karger AG, Basel
- Wilhelm KP, Anastasia B, Maibach HI: Skin ageing. Arch Dermatol 1991;127:1806–1809.
Rawlings AV, Rogers J, Mayo AM: Changes in lipids in the skin aging process. Biocosmet Skin Aging 1993;1:31–45.
- Imokawa G, Akasaki S, Minematsu Y, Kawai M: Importance of intercellular lipids in water-retention properties of the stratum corneum: Induction and recovery study of surfactant dry skin. Arch Dermatol Res 1989;281:45–51.
- Denda M, Koyama J, Hori J, Horii I, Takahashi M, Hara M, Tagami H: Age- and sex-dependent change in stratum corneum sphingolipids. Arch Dermatol Res 1993;285:415–417.
- Ghadially RG, Brown BE, Sequeira-Martin SM, Feingold KR, Elias PM: The aged epidermal permeability barrier: Structural, functional, and lipid biochemical abnormalities in humans and a senescent murine model. J Clin Invest 1995;95:2281–2290.
- Yamamoto A, Serizawa S, Ito M, Sato Y: Stratum corneum lipid abnormalities in atopic dermatitis. Arch Dermatol Res 1991;283:219–223.
- Motta S, Monti M, Sesana S, Mellesi L, Ghidoni R, Caputo R: Abnormality of water barrier function in psoriasis: Role of ceramide fractions. Arch Dermatol 1994;130:452–456.
- Paige DG, Morse-Fischer N, Harper JI: Quantification of stratum corneum ceramides and lipid envelope ceramides in the hereditary ichthyoses. Br J Dermatol 1994;131:23–27.
- Di Nardo A, Wertz P, Giannetti A, Seidenari S: Ceramide and cholesterol composition of the skin of patients with atopic dermatitis. Acta Derm Venereol 1998;78:27–30.
De Paepe K, Rogiers V: Analytical techniques for skin lipids; in Förster T (ed): Cosmetic Lipids and the Skin Barrier. London, Marcel Dekker, 2001, pp 149–184.
- Zellmer S, Lasch J: Individual variation of human plantar SC lipids, determined by automated multiple development of high-performance thin-layer chromatography plates. J Chromatogr 1997;691:321–329.
Hood HL, Wickett RR: Racial differences in epidermal structure and function. Cosmet Toiletries 1992;107:47–48.
- Lavrijsen AP, Higounec IM, Weerheim A, Oestmann E, Tuinenburg EE, Boddé HE, Ponec M: Validation of an in vivo extraction method for stratum corneum ceramides. Arch Dermatol Res 1994;286:495–503.
- Conti A, Rogers J, Verdejo P, Harding CR, Rawlings AV: Seasonal influences on stratum corneum ceramide 1 fatty acids and the influence of topical essential fatty acids. Int J Cosmet Sci 1996;18:1–12.
- Wefers H, Melnik BC, Flür M, Bluhm C, Lehmann P, Plewig G: Influence of UV irradiation on the composition of human SC lipids. J Invest Dermatol 1991;96:959–962.
- Lampe MA, Burlingame AL, Whitney J, Williams ML, Brown BE, Roitman E, Elias PM: Human SC lipids: Characterization and regional variations. J Lipid Res 1983;24:120–130.
- Lampe MA, Williams ML, Elias PM: Human epidermal lipids: Characterization and modulation during differentiation. J Lipid Res 1983;24:131–140.
- Williams ML, Elias PM: N-alkanes in normal and pathological human scale. Biochem Biophys Res Commun 1982;107:322–328.
Bonté F, Pinguet P, Chevalier J, Meybeck A: Analysis of all stratum corneum lipids by automated multiple development high-performance thin-layer chromatography. J Chromatogr B Biomed Appl 1995;664:311–316.
- Melnik BC, Hollmann J, Erler E, Verhoeven B, Plewig G: Micro-analytical screening of all major stratum corneum lipids by sequential high-performance thin-layer chromatography. J Invest Dermatol 1989;92:231–234.
- Fulmer AW, Kramer GJ: Stratum corneum lipid abnormalities in surfactant-induced dry scaly skin. J Invest Dermatol 1986;86:598–602.
- Norlén L, Nicander I, Lundsjö A, Cronholm T, Forslind B: A new HPLC-based method for the quantitative analysis of inner stratum corneum lipids with special reference to the free fatty acid fraction. Arch Dermatol Res 1998;290:508–516.
- Ponec M, Weerheim A: Retinoids and lipid changes in keratinocytes. Methods Enzymol 1990;190:30–41.
- Lavrijsen AP, Bouwstra JA, Gooris GS, Weerheim A, Boddé HE, Ponec M: Reduced skin barrier function parallels abnormal stratum corneum lipid organization in patients with lamellar ichthyosis. J Invest Dermatol 1995;106:619–624.
- Robson KJ, Stewart ME, Michelsen S, Lazo ND, Downing DT: 6-Hydroxy-4-sphingenine in human epidermal ceramides. J Lipid Res 1994;35:2060–2068.
- Rawlings AV, Watkinson A, Rogers J, Mayo AM, Hope J, Scott IR: Abnormalities in stratum corneum structure, lipid composition, and desmosome degradation in soap-induced winter xerosis. J Soc Cosmet Chem 1994;45:203–220.
- Kiistala U: The influence of age, sex and body region on suction blister formation in human skin. Ann Clin Res 1972;4:236–246.
- Michiyuki K: Epidermal grafting using the tops of suction blisters in the treatment of vitiligo. Arch Dermatol 1988;124:1656–1658.
- Röpke EM, Augustin W, Gollnick H: Improved method for studying skin lipid samples from cyanoacrylate strips by high-performance thin-layer chromatography. Skin Pharmacol 1996;9:381–387.
- Rogers J, Harding CR, Mayo AM, Banks J, Rawlings AV: Stratum corneum lipids: The effect of ageing and the seasons. Arch Dermatol Res 1996;288:765–770.
- Zettersten E, Ghadially R, Feingold K, Crumrine D, Elias PM: Optimal ratios of topical stratum corneum lipids improve barrier recovery in chronologically aged skin. J Am Acad Dermatol 1997;37:403–408.
- Reed JT, Ghadially R, Elias PM: Skin type, but neither race nor gender, influence epidermal permeability barrier function. Arch Dermatol 1995;131:1134–1138.
- Kompaore F, Marty JP, Dupont C: In vivo evaluation of the stratum corneum barrier function in blacks, Caucasians and Asians with two non-invasive methods. Skin Pharmacol 1993;6:200–207.
Rawlings AV, Harding CR, Schilling KM: Ceramides and the skin; in Baran R, Maibach HI (eds): Textbook of Cosmetic Dermatology, ed 2. London, Dunitz, 1998, pp 99–112.
- Jass HE, Elias PM: The living stratum corneum: Implications for cosmetic formulation. Cosmet Toiletries 1991;106:47–53.
- Dreher F, Arens A, Hostynek JJ, Mudumba S, Ademola J, Maibach HI: Colorimetric method for quantifying human stratum corneum removed by adhesive-tape stripping. Acta Derm Venereol 1998;78:186–189.
Schaefer H, Redelmeier TE (eds): Skin Barrier: Principles of Percutaneous Absorption. Basel, Karger, 1996, pp 165–183, 198–207.
- Weerheim A, Ponec M: Determination of stratum corneum lipid profile by tape stripping in combination with high-perfomance thin-layer chromatography. Arch Dermatol Res 2001;293:191–199.
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