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Original Paper

Analysis of Epidermal Lipids of the Healthy Human Skin: Factors Affecting the Design of a Control Population

De Paepe K.a · Weerheim A.c · Houben E.a · Roseeuw D.b · Ponec M.c · Rogiers V.a

Author affiliations

Departments of aToxicology and Dermato-Cosmetology and bDermatology, Vrije Universiteit Brussel, Brussels, Belgium; cSkin Research Laboratory, Leiden University Medical Center, Leiden, The Netherlands

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Skin Pharmacol Physiol 2004;17:23–30

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 30, 2003
Accepted: December 11, 2003
Published online: December 22, 2003
Issue release date: January – February

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 4

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: https://www.karger.com/SPP

Abstract

The intervariability of studies on the lipids of human epidermis and stratum corneum is high because of the different origin of the skin samples and the variety of extraction methods used. In the present work, a high-performance thin-layer chromatographic technique has been used to study the parameters age, sex, and anatomical site for their effects on the lipid profiles recovered from healthy epidermal skin biopsy specimens. It was found that sex-related differences were seen at the level of the total ceramide concentration. Observed decreases in lipid concentration, due to ageing, depended on the anatomical site. Therefore, these variables should be controlled in a reproducible and standardized way in order to be able to study the direct relationship between skin condition and barrier lipid composition. Only when this relation is established, results of topical treatment can be scientifically evaluated.

© 2004 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 30, 2003
Accepted: December 11, 2003
Published online: December 22, 2003
Issue release date: January – February

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 4

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: https://www.karger.com/SPP


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