International Archives of Allergy and Immunology

Original Paper

Cytokine Production, Lymphocyte Proliferation and T-Cell Receptor Vβ Expression in Primary Peripheral Blood Mononuclear Cell Cultures from Nickel-Allergic Individuals

Cederbrant K.a,c · Anderson C.b · Andersson T.b,† · Marcusson-Ståhl M.c · Hultman P.a

Author affiliations

aDepartment of Molecular and Clinical Medicine, Division of Molecular and Immunological Pathology (AIR), bDepartment of Biomedicine and Surgery, Division of Dermatology, Linköping University, Linköping, and cAstraZeneca R&D Södertälje, Safety Assessment, Södertälje, Sweden

Keywords: Nickel allergyLymphocyte transformation testInterleukin-10T-cell receptor Vβ17

Related Articles for ""
Int Arch Allergy Immunol 2003;132:373–379
https://doi.org/10.1159/000074905

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: April 08, 2003
Accepted: August 14, 2003
Published online: January 14, 2004
Issue release date: December 2003

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: Clinical history and patch test constitute the two cornerstones in the diagnosis of nickel (Ni) allergy. Due to technical and interpretative limits of the patch test, the in vitro lymphocyte transformation test (LTT) has been developed for confirming contact allergy; however, most studies show an overlap in lymphocyte proliferation between Ni-allergic and nonallergic subjects using the LTT. The aim of this study was to see if the secretion of cytokines, especially interleukin (IL)-10 and IL-17, or the use of T-cell receptor (TCR) Vβ families in Ni-stimulated primary peripheral blood mononuclear cell (PBMC) cultures might be more useful for discriminating between allergic and nonallergic subjects. Methods: Ni2+-stimulated primary PBMC cultures derived from female subjects diagnosed as Ni-allergic (n = 5) or nonallergic (n = 5) on the basis of a positive or negative patch test were assessed for cell proliferation by tritiated thymidine incorporation and for production of interferon-γ, IL-4, IL-10 and IL-17 in the culture supernatant by ELISA. The immunophenotype and TCR-Vβ family affiliation of the Ni2+-induced lymphoblasts were determined by flow cytometry. Results: Lymphocytes from Ni-allergic individuals challenged with a high and a low concentration of Ni showed significantly higher cell proliferation than lymphocytes from nonallergic individuals, but all subjects showed a positive LTT result (stimulation index >2). We found a significantly higher release of IL-10 in Ni2+-treated cultures from Ni-allergic compared with nonallergic subjects that provided better separation between individuals in the two groups than did lymphocyte proliferation. The proliferating lymphoblasts were predominantly CD4+, and in 2 of the 5 Ni-allergic subjects, but in none of the 5 nonallergic subjects, the CD4+ lymphoblasts showed a dominance of TCR-Vβ17. Conclusions: Determination of IL-10 production in primary PBMC cultures is a potentially promising in vitro method for discrimination of Ni allergy in females, as compared with cell proliferation.

© 2003 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: April 08, 2003
Accepted: August 14, 2003
Published online: January 14, 2004
Issue release date: December 2003

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

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2003, Vol.132, No. 4

December 2003

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†Dr. Andersson died on January 30, 2003.
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