A canine cancer-gene microarray for CGH analysis of tumorsThomas R.a,b · Fiegler H.c · Ostrander E.A.d · Galibert F.e · Carter N.P.c · Breen M.b
aOncology Research Group, Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk (UK); bDepartment of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC (USA); cThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge (UK); dClinical Research and Human Biology Divisions, Fred Hutchinson Cancer Research Center, Seattle WA (USA); eUMR 6061 CNRS, Génétique et développement, Faculté de Médecine, Rennes (France)
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As with many human cancers, canine tumors demonstrate recurrent chromosome aberrations. A detailed knowledge of such aberrations may facilitate diagnosis, prognosis and the selection of appropriate therapy. Following recent advances made in human genomics, we are developing a DNA microarray for the domestic dog, to be used in the detection and characterization of copy number changes in canine tumors. As a proof of principle, we have developed a small-scale microarray comprising 87 canine BAC clones. The array is composed of 26 clones selected from a panel of 24 canine cancer genes, representing 18 chromosomes, and an additional set of clones representing dog chromosomes 11, 13, 14 and 31. These chromosomes were shown previously to be commonly aberrant in canine multicentric malignant lymphoma. Clones representing the sex chromosomes were also included. We outline the principles of canine microarray development, and present data obtained from microarray analysis of three canine lymphoma cases previously characterized using conventional cytogenetic techniques.
© 2003 S. Karger AG, Basel
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