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Original Article

Multitude multicolor chromosome banding (mMCB) – a comprehensive one-step multicolor FISH banding method

Weise A.a · Heller A.a · Starke H.a · Mrasek K.a · Kuechler A.a,b · Pool-Zobel B.L.c · Claussen U.a · Liehr T.a

Author affiliations

aInstitute of Human Genetics and Anthropology and Departments of bRadiation Oncology, cNutritional Toxicology, Institute of Nutrition, University of Jena, Jena (Germany)

Related Articles for ""

Cytogenet Genome Res 103:34–39 (2003)

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: August 26, 2003
Accepted: October 16, 2003
Published online: March 30, 2004
Issue release date: 2003

Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Multicolor chromosome banding (MCB) using one single chromosome-specific MCB probe set per experiment was previously reported as powerful tool in molecular cytogenetics for the characterization of all kinds of human marker chromosomes. However, a quick analysis of karyotypes with highly complex chromosomal changes was hampered by the problem that up to 24 MCB experiments were necessary for a comprehensive karyotype description. To overcome that limitation the 138 available region-specific microdissection-derived libraries for all human chromosomes were combined to one single probe set, called multitude MCB (mMCB). A typical fluorescence banding pattern along the human karyotype is produced, which can be evaluated either by transforming these profiles into chromosome region-specific pseudo-colors or more reliably by studying the fluorescence profiles. The mMCB probe set has been applied on chromosomes of normal male and female probands, two primary myelodysplastic syndromes and two solid tumor cell lines. Additionally, a cell line of Gorilla gorilla (GGO) studied previously by single chromosome-specific MCB was reevaluated by the mMCB method. All results were in concordance with those obtained in parallel or by other cytogenetic and molecular cytogenetic approaches indicating that mMCB is a powerful multicolor FISH banding tool for fast characterization of complex karyotypes.   

© 2003 S. Karger AG, Basel


References

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    External Resources
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Article / Publication Details

First-Page Preview
Abstract of Original Article

Received: August 26, 2003
Accepted: October 16, 2003
Published online: March 30, 2004
Issue release date: 2003

Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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