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Animal Cytogenetics and Comparative Mapping

Seven-fluorochrome mouse M-FISH for high-resolution analysis of interchromosomal rearrangements

Jentsch I.a,b · Geigl J.c · Klein C.A.c · Speicher M.R.a,b

Author affiliations

aInstitut für Humangenetik, Technische Universität München, München (Germany); bInstitut für Humangenetik, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg (Germany); cInstitut für Immunologie, Ludwig-Maximilians-Universität München, München (Germany)

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Cytogenet Genome Res 103:84–88 (2003)

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Article / Publication Details

First-Page Preview
Abstract of Animal Cytogenetics and Comparative Mapping

Published online: March 30, 2004
Issue release date: 2003

Number of Print Pages: 5
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

The mouse has evolved to be the primary mammalian genetic model organism. Important applications include the modeling of human cancer and cloning experiments. In both settings, a detailed analysis of the mouse genome is essential. Multicolor karyotyping technologies have emerged to be invaluable tools for the identification of mouse chromosomes and for the deciphering of complex rearrangements. With the increasing use of these multicolor technologies resolution limits are critical. However, the traditionally used probe sets, which employ 5 different fluorochromes, have significant limitations. Here, we introduce an improved labeling strategy. Using 7 fluorochromes we increased the sensitivity for the detection of small interchromosomal rearrangements (700 kb or less) to virtually 100%. Our approach should be important to unravel small interchromosomal rearrangements in mouse models for DNA repair defects and chromosomal instability.   

© 2003 S. Karger AG, Basel


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    External Resources
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Article / Publication Details

First-Page Preview
Abstract of Animal Cytogenetics and Comparative Mapping

Published online: March 30, 2004
Issue release date: 2003

Number of Print Pages: 5
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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