Gynecologic and Obstetric Investigation
Original Article
Effects of Diazeniumdiolates (NONOates) and Methylene Blue on the Reduction of Postoperative Adhesion in RatsCetin M. · Duran B. · Demirkoprulu N. · Guvenal T. · Erden O. · Cetin A.Department of Obstetrics and Gynecology, Cumhuriyet University School of Medicine, Sivas, Turkey
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Article / Publication Details
Received: October 31, 2003
Accepted: December 09, 2003
Published online: May 13, 2004
Issue release date: May 2004
Number of Print Pages: 5
Number of Figures: 1
Number of Tables: 1
ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)
For additional information: https://www.karger.com/GOI
Abstract
We investigated the effects of spermine NONOate (SPER/NO), diethylenetriamine NONOate (DETA/NO) and methylene blue for preventing postoperative adhesion in a rat uterine horn model. Before operations, rats were randomly assigned into 6 groups, each composed of 12 rats. These were the sham, control, normal saline, SPER/NO, DETA/NO and methylene blue groups. Each rat was anesthetized with ketamine hydrochloride (40 mg/kg i.v.). The abdominal wall was shaved; the surgical site was scrubbed with povidone iodine and rinsed with sterile saline 3 times before surgery. Under sterile conditions, a 3-cm vertical midline incision was made in all groups. In the sham group, we closed the abdominal wall without any procedure. In the other groups, a 2-cm segment of each uterine horn was injured in 10 spots on the antimesenteric surface using unipolar cautery. Before the final abdominal closure, no adjuvant therapy was administered intraperitoneally to the rats in the control group; 2 ml of normal saline solution, 1% methylene blue solution, SPER/NO (0.5 mg/ml) and DETA/NO (0.1 mg/ml) were instilled into the uterine horns of the rats in the respective groups. The incision was closed with a running 4-0 monofilament delayed absorbable suture in a single layer of muscle and fascia in a running pattern, excluding the peritoneum, and in the covering layer of skin in an interrupted pattern, in all groups. Two weeks after the surgery, all animals were killed, second laparotomies were performed, and the extent and severity of adhesions were determined by a blinded examiner. The adhesion scores of the sham group were significantly lower than those of the other groups (p < 0.05). In the methylene blue, SPER/NO and DETA/NO groups, adhesion scores were significantly lower than in the normal saline and control groups (p < 0.05). However, there were no significant differences related to the extent and severity scores of adhesions between the methylene blue, SPER/NO and DETA/NO groups (p > 0.05). This study showed that SPER/NO, DETA/NO and methylene blue administered at the end of surgery reduced the adhesion formation in a rat uterine horn model.
© 2004 S. Karger AG, Basel
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References
-
Monk BJ, Berman ML, Montz FJ: Adhesions after extensive gynecologic surgery: Clinical significance, etiology, and prevention. Am J Obstet Gynecol 1994;170(5, pt 1):1396–1403.
-
Menzies D: Postoperative adhesions: Their treatment and relevance in clinical practice. Ann R Coll Surg Engl 1993;75:147–153.
External Resources
-
Holtz G: Prevention and management of peritoneal adhesions. Fertil Steril 1984;41:497–507.
External Resources
- Thompson JN, Whawell SA: Pathogenesis and prevention of adhesion formation. Br J Surg 1995;82:3–5.
- Yaacobi Y, Israel AA, Goldberg EP: Prevention of postoperative abdominal adhesions by tissue precoating with polymer solutions. J Surg Res 1993;55:422–426.
- Linsky CB, Diamond MP, Cunningham T, Constantine B, De Cherney AH, di Zerega GS: Adhesion reduction in the rabbit uterine horn model using an absorbable barrier TC-7. J Reprod Med 1987;32:17–20.
-
Guvenal T, Cetin A, Ozdemir H, Yanar O, Kaya T: Prevention of postoperative adhesion formation in rat uterine horn model by nimesulide: A selective COX-2 inhibitor. Hum Reprod 2001;16:1732–1735.
External Resources
- Sahin Y, Saglam A: Synergistic effects of carboxymethylcellulose and low molecular weight heparin in reducing adhesion formation in the rat uterine horn model. Acta Obstet Gynecol Scand 1994;73:70–73.
-
Steinleitner A, Kazensky C, Lambert H: Calcium channel blockade prevents postsurgical reformation of adnexal adhesions in rabbits. Obstet Gynecol 1989;74:796–804.
External Resources
- Galili Y, Ben-Abraham R, Rabau M, Klausner J, Kluger Y: Reduction of surgery-induced peritoneal adhesions by methylene blue. Am J Surg 1998;175:30–32.
-
Ozden A, Bostanci B, Sarioglu A, Taskiran D, Tetik C: Effect of nitric oxide on postoperative adhesion formation. Eur Surg Res 1999;31:465–470.
External Resources
-
Duran B, Ak D, Cetin A, Guvenal T, Cetin M, Imir AG: Reduction of postoperative adhesions by N,O-carboxymethylchitosan and spermine NONOate in rats. Exp Anim Tokyo 2003;52:267–272.
- Homer K, Wanstall J: In vitro comparison of two NONOates (novel nitric oxide donors) on rat pulmonary arteries. Eur J Pharmacol 1998;356:49–57.
- Lomniczi A, Cebral E, Canteros G, McCann SM, Rettori V: Methylene blue inhibits the increase of inducible nitric oxide synthase activity induced by stress and lipopolysaccharide in the medial basal hypothalamus of rats. Neuroimmunomodulation 2000;8:122–127.
- Rodeberg DA, Chaet MS, Bass RC, Arkovitz MS, Garcia VF: Nitric oxide: An overview. Am J Surg 1995;170:292–303.
- Billiar TR: Nitric oxide: Novel biology with clinical relevance. Ann Surg 1995;221:339–349.
- Masini E, Bianchi S, Mugnai L, Gambassi F, Lupini M, Pistelli A, Mannaioni PF: The effect of nitric oxide generators on ischemia reperfusion injury and histamine release in isolated perfused guinea-pig heart. Agents Actions 1991;33:53–56.
- Ellis H: The cause and prevention of postoperative intraperitoneal adhesions. Surg Gynecol Obstet 1971;133:497–511.
- Vipond MN, Whawell SA, Thompson JN, Dudley HA: Peritoneal fibrinolytic activity and intra-abdominal adhesions. Lancet 1990;335:1120–1122.
- Liebman SM, Langer JC, Marshall JS, Collins SM: Role of mast cells in peritoneal adhesion formation. Am J Surg 1993;165:127–130.
- Chegini N, Gold LI, Williams RS, Masterson BJ: Localization of transforming growth factor beta isoforms TGF-beta 1, TGF-beta 2, and TGF-beta 3 in surgically induced pelvic adhesions in the rat. Obstet Gynecol 1994;83:449–454.
-
Ekerhovd E, Brannstrom M, Delbro D, Norstrom A: Nitric oxide mediated inhibition of contractile activity in the human uterine cervix. Mol Hum Reprod 1998;4:915–920.
External Resources
- Du ZY, Hicks M, Jansz P, Rainer S, Spratt P, Macdonald P: The nitric oxide donor, diethylamine NONOate, enhances preservation of the donor rat heart. J Heart Lung Transplant 1998;17:1113–1120.
- Salaris SC, Babbs CF, Voorhees WD 3rd: Methylene blue as an inhibitor of superoxide generation by xanthine oxidase: A potential new drug for the attenuation of ischemia/reperfusion injury. Biochem Pharmacol 1991;42:499–506.
-
Kluger Y, Weinbroum A, Ben-Avraham R, Galili Y, Klausner J, Rabau M: Reduction in formation of peritoneal adhesions by methylene blue in rats: A dose response study. Eur J Surg 2000;166:568–571.
External Resources
Article / Publication Details
Received: October 31, 2003
Accepted: December 09, 2003
Published online: May 13, 2004
Issue release date: May 2004
Number of Print Pages: 5
Number of Figures: 1
Number of Tables: 1
ISSN: 0378-7346 (Print)
eISSN: 1423-002X (Online)
For additional information: https://www.karger.com/GOI
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