Login to MyKarger

New to MyKarger? Click here to sign up.



Login with Facebook

Forgot your password?

Authors, Editors, Reviewers

For Manuscript Submission, Check or Review Login please go to Submission Websites List.

Submission Websites List

Institutional Login
(Shibboleth or Open Athens)

For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.

Pathways of post-transcriptional gene regulation in mammalian germ cell development

Author affiliations

Institute of Human Genetics, University of Newcastle upon Tyne, Newcastle (England)

Related Articles for ""

Cytogenet Genome Res 103:210–216 (2003)

Do you have an account?

Login Information





Contact Information












By signing up for MyKarger you will automatically participate in our year-End raffle.
If you Then Do Not wish To participate, please uncheck the following box.

Yes, I wish To participate In the year-End raffle And Get the chance To win some Of our most interesting books, And other attractive prizes.


I have read the Karger Terms and Conditions and agree.



Login Information





Contact Information












By signing up for MyKarger you will automatically participate in our year-End raffle.
If you Then Do Not wish To participate, please uncheck the following box.

Yes, I wish To participate In the year-End raffle And Get the chance To win some Of our most interesting books, And other attractive prizes.


I have read the Karger Terms and Conditions and agree.



To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.


Save over 20% compared to the individual article price.
Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00


Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Paper

Received: September 11, 2003
Accepted: November 14, 2003
Published online: March 30, 2004
Issue release date: 2003

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Male germ cell development is orchestrated by complex and disparate patterns of gene expression operating in different cell types. The mechanisms of gene expression underlying these have been dissected in the mouse because of its readily available genetics. These analyses have shown that as well as the traditional transcriptional mechanisms, post-transcriptional regulatory pathways of gene expression are essential for mouse spermatogenesis. Proteins essential for germ cell development have been identified which operate at different points throughout the life cycle of RNA from pre-mRNA splicing to translation and RNA decay in the cytoplasm. Recent data suggests that these post-transcriptional pathways respond to environmental cues via signalling pathways.   

© 2003 S. Karger AG, Basel


References

  1. Akagi T, Kamei D, Tsuchiya N, Nishina Y, Horiguchi H, Matsui M, Kamma H, Yamada M: Molecular characterization of a mouse heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its tissue-specific expression.Gene 245:267–273 (2000).
  2. Beck AR, Medley QG, O’Brien S, Anderson P, Streuli M: Structure, tissue distribution and genomic organization of the murine RRM-type RNA binding proteins TIA-1 and TIAR. Nucl Acids Res 24:3829–3835 (1996).
  3. Beck AR, Miller IJ, Anderson P, Streuli M:RNA-binding protein TIAR is essential for primordial germ cell development. Proc natl Acad Sci, USA 95:2331–2336 (1998).
  4. Chen MS, Hurov J, White LS, Woodford-Thomas T, Piwnica-Worms H: Absence of apparent phenotype in mice lacking Cdc25C protein phosphatase. Mol Cell Biol 21:3853–3861 (2001).
  5. Cooke HJ, Lee M, Kerr S, Ruggiu M: A murine homologue of the human DAZ gene is autosomal and expressed only in male and female gonads. Hum molec Genet 5:513–516 (1996).
  6. Dass B, McMahon KW, Jenkins NA, Gilbert DJ, Copeland NG, MacDonald CC: The gene for a variant form of the polyadenylation protein CstF-64 is on chromosome 19 and is expressed in pachytene spermatocytes in mice. J biol Chem 276:8044–8050 (2001).
  7. Delbridge ML, Lingenfelter PA, Disteche CM, Graves JA: The candidate spermatogenesis gene RBMY has a homologue on the human X chromosome. Nature Genet 22:223–224 (1999).
  8. Deng W, Lin H: miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell 2:819–830 (2002).
  9. Elliott DJ, Cooke HJ: The molecular genetics of male infertility. Bioessays 19:801–809 (1997).
  10. Elliott DJ, Ma K, Kerr SM, Thakrar R, Speed R, Chandley AC, Cooke H: An RBM homologue maps to the mouse Y chromosome and is expressed in germ cells. Hum molec Genet 5:869–874 (1996).
  11. Elliott DJ, Bourgeois CF, Klink A, Stevenin J, Cooke HJ: A mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection. Proc natl Acad Sci, USA 97:5717–5722 (2000a).
  12. Elliott DJ, Venables JP, Newton CS, Lawson D, Boyle S, Eperon IC, Cooke HJ: An evolutionarily conserved germ cell-specific hnRNP is encoded by a retrotransposed gene. Hum molec Genet 9:2117–2124 (2000b).
  13. Feng LX, Chen Y, Dettin L, Pera RA, Herr JC, Goldberg E, Dym M: Generation and in vitro differentiation of a spermatogonial cell line. Science 297:392–395 (2002).
  14. Forch P, Valcarcel J: Molecular mechanisms of gene expression regulation by the apoptosis-promoting protein TIA-1. Apoptosis 6:463–468 (2001).
  15. Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J: The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing. Mol Cell 6:1089–1098 (2000).
  16. Forch P, Puig O, Martinez C, Seraphin B, Valcarcel J: The splicing regulator TIA-1 interacts with U1-C to promote U1 snRNP recruitment to 5′ splice sites. Embo J 21:6882–6892 (2002).
  17. Foulkes NS, Mellstrom B, Benusiglio E, Sassone-Corsi P: Developmental switch of CREM function during spermatogenesis: from antagonist to activator. Nature 355:80–84 (1992).
  18. Foulkes NS, Schlotter F, Pevet P, Sassone-Corsi P: Pituitary hormone FSH directs the CREM functional switch during spermatogenesis. Nature 362:264–267 (1993).
  19. Gebauer F, Hentze MW: Fertility facts: male and female germ cell development requires translational control by CPEB. Mol Cell 8:247–249 (2001).
  20. Giorgini F, Davies HG, Braun RE: MSY2 and MSY4 bind a conserved sequence in the 3′ untranslated region of protamine 1 mRNA in vitro and in vivo. Mol Cell Biol 21:7010–7019 (2001).
  21. Giorgini F, Davies HG, Braun RE: Translational repression by MSY4 inhibits spermatid differentiation in mice. Development 129:3669–3679 (2002).
  22. Hanamura A, Caceres JF, Mayeda A, Franza BR Jr, Krainer AR: Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. Rna 4:430–444 (1998).
  23. Kamma H, Portman DS, Dreyfuss G: Cell type-specific expression of hnRNP proteins. Exp Cell Res 221:187–196 (1995).
  24. Kamma H, Horiguchi H, Wan L, Matsui M, Fujiwara M, Fujimoto M, Yazawa T, Dreyfuss G: Molecular characterization of the hnRNP A2/B1 proteins: tissue-specific expression and novel isoforms. Exp Cell Res 246:399–411 (1999).
  25. Kashiwabara S, Zhuang T, Yamagata K, Noguchi J, Fukamizu A, Baba T: Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells. Dev Biol 228:106–115 (2000).
  26. Kashiwabara S, Noguchi J, Zhuang T, Ohmura K, Honda A, Sugiura S, Miyamoto K, Takahashi S, Inoue K, Ogura A, et al: Regulation of spermatogenesis by testis-specific, cytoplasmic poly(A) polymerase TPAP. Science 298:1999–2002 (2002).
  27. Kuroda M, Sok J, Webb L, Baechtold H, Urano F, Yin Y, Chung P, de Rooij DG, Akhmedov A, Ashley T, et al: Male sterility and enhanced radiation sensitivity in TLS(–/–) mice. EMBO J 19:453–462 (2000).
  28. Le Guiner C, Lejeune F, Galiana D, Kister L, Breathnach R, Stevenin J, Del Gatto-Konczak F: TIA-1 and TIAR activate splicing of alternative exons with weak 5′ splice sites followed by a U-rich stretch on their own pre-mRNAs. J biol Chem 276:40638–40646 (2001).
  29. Lee K, Fajardo MA, Braun RE: A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor. Mol Cell Biol 16:3023–3034 (1996).
  30. Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F: Identification of an RNA binding specificity for the potential splicing factor TLS. J biol Chem 276:6807–6816 (2001).
  31. Mahadevaiah SK, Odorisio T, Elliott DJ, Rattigan A, Szot M, Laval SH, Washburn LL, McCarrey JR, Cattanach BM, Lovell-Badge R, et al: Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum molec Genet 7:715–727 (1998).
    External Resources
  32. Maines JZ, Wasserman SA: Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the Dazl orthologue Boule. Nature Cell Biol 1:171–174 (1999).
  33. Maniatis T, Reed R: An extensive network of coupling among gene expression machines. Nature 416:499–506 (2002).
  34. Maniatis T, Tasic B: Alternative pre-mRNA splicing and proteome expansion in metazoans. Nature 418:236–243 (2002).
  35. Matter N, Herrlich P, Konig H: Signal-dependent regulation of splicing via phosphorylation of Sam68. Nature 420:691–695 (2002).
  36. Mazeyrat S, Saut N, Grigoriev V, Mahadevaiah SK, Ojarikre OA, Rattigan A, Bishop C, Eicher EM, Mitchell MJ, Burgoyne PS: A Y-encoded subunit of the translation initiation factor Eif2 is essential for mouse spermatogenesis. Nature Genet 29:49–53 (2001).
  37. Meissner M, Lopato S, Gotzmann J, Sauermann G, Barta A: Proto-oncoprotein TLS/FUS is associated to the nuclear matrix and complexed with splicing factors PTB, SRm160, and SR proteins. Exp Cell Res 283:184–195 (2003).
  38. Misteli T, Caceres JF, Clement JQ, Krainer AR, Wilkinson MF, Spector DL: Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. J Cell Biol 143:297–307 (1998).
  39. Mitchell P, Tollervey D: mRNA turnover. Curr Opin Cell Biol 13:320–325 (2001).
  40. Moore FL, Jaruzelska J, Fox MS, Urano J, Firpo MT, Turek PJ, Dorfman DM, Pera RA: Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in Azoospermia) and DAZ-like proteins. Proc natl Acad Sci, USA 100:538–543 (2003).
  41. Nantel F, Monaco L, Foulkes NS, Masquilier D, LeMeur M, Henriksen K, Dierich A, Parvinen M, Sassone-Corsi P: Spermiogenesis deficiency and germ-cell apoptosis in CREM-mutant mice. Nature 380:159–162 (1996).
  42. Nasim MT, Chernova TK, Chowdhury HM, Yue BG, Eperon IC: HnRNP G and Tra2beta: opposite effects on splicing matched by antagonism in RNA binding. Hum molec Genet 12:1337–1348 (2003).
  43. Okazaki Y, Furuno M, Kasukawa T, Adachi J, Bono H, Kondo S, Nikaido I, Osato N, Saito R, Suzuki H, et al: Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature 420:563–573 (2002).
  44. Papoutsopoulou S, Nikolakaki E, Chalepakis G, Kruft V, Chevaillier P, Giannakouros T: SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1. Nucl Acids Res 27:2972–2980 (1999).
  45. Perrotti D, Bonatti S, Trotta R, Martinez R, Skorski T, Salomoni P, Grassilli E, Lozzo RV, Cooper DR, Calabretta B: TLS/FUS, a pro-oncogene involved in multiple chromosomal translocations, is a novel regulator of BCR/ABL-mediated leukemogenesis. EMBO J 17:4442–4455 (1998).
  46. Powledge TM: Bear market slashes at human genome. The dropping guesses about the number of human genes challenges researchers to explain human complexity with so few genes. EMBO Rep 1:212–214 (2000).
  47. Roberts GC, Smith CW: Alternative splicing: combinatorial output from the genome. Curr Opin Chem Biol 6:375–383 (2002).
  48. Ruggiu M, Speed R, Taggart M, McKay SJ, Kilanowski F, Saunders P, Dorin J, Cooke HJ: The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis. Nature 389:73–77 (1997).
  49. Ruggiu M, Saunders PT, Cooke HJ: Dynamic subcellular distribution of the DAZL protein is confined to primate male germ cells. J Androl 21:470–477 (2000).
  50. Sassone-Corsi P: Transcriptional checkpoints determining the fate of male germ cells. Cell 88:163–166 (1997).
  51. Schumacher JM, Lee K, Edelhoff S, Braun RE: Distribution of Tenr, an RNA-binding protein, in a lattice-like network within the spermatid nucleus in the mouse. Biol Reprod 52:1274–1283 (1995).
  52. Schumacher JM, Lee K, Edelhoff S, Braun RE: Spnr, a murine RNA-binding protein that is localized to cytoplasmic microtubules. J Cell Biol 129:1023–1032 (1995).
  53. Shatkin AJ, Manley JL: The ends of the affair: capping and polyadenylation. Nature Struct Biol 7:838–842 (2000).
  54. Slee R, Grimes B, Speed RM, Taggart M, Maguire SM, Ross A, McGill NI, Saunders PT, Cooke HJ: A human DAZ transgene confers partial rescue of the mouse Dazl null phenotype. Proc natl Acad Sci, USA 96:8040–8045 (1999).
  55. Smith CW, Valcarcel J: Alternative pre-mRNA splicing: the logic of combinatorial control. Trends Biochem Sci 25:381–388 (2000).
  56. Stoss O, Olbrich M, Hartmann AM, Konig H, Memmott J, Andreadis A, Stamm S: The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites. J biol Chem 276:8665–8673 (2001).
  57. Tanaka SS, Toyooka Y, Akasu R, Katoh-Fukui Y, Nakahara Y, Suzuki R, Yokoyama M, Noce T: The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev 14:841–853 (2000).
  58. Tay J, Richter JD: Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice. Dev Cell 1:201–213 (2001).
  59. Toyooka Y, Tsunekawa N, Takahashi Y, Matsui Y, Satoh M, Noce T: Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development. Mech Dev 93:139–149 (2000).
  60. Venables JP: Alternative splicing in the testes. Curr Opin Genet Dev 12:615–619 (2002).
  61. Venables JP, Cooke HJ: Lessons from knockout and transgenic mice for infertility in men. J Endocrinol Invest 23:584–591 (2000).
  62. Venables JP, Vernet C, Chew SL, Elliott DJ, Cowmeadow RB, Wu J, Cooke HJ, Artzt K, Eperon IC: T-STAR/ETOILE: a novel relative of SAM68 that interacts with an RNA-binding protein implicated in spermatogenesis. Hum molec Genet 8:959–969 (1999).
  63. Venables JP, Ruggiu M, Cooke HJ: The RNA-binding specificity of the mouse Dazl protein. Nucl Acids Res 29:2479–2483 (2001).
  64. Vogel T, Speed RM, Ross A, Cooke HJ: Partial rescue of the Dazl knockout mouse by the human DAZL gene. Mol Hum Reprod 8:797–804 (2002).
  65. Wallace AM, Dass B, Ravnik SE, Tonk V, Jenkins NA, Gilbert DJ, Copeland NG, MacDonald CC: Two distinct forms of the 64,000 Mr protein of the cleavage stimulation factor are expressed in mouse male germ cells. Proc natl Acad Sci, USA 96:6763–6768 (1999).
  66. Xu EY, Moore FL, Pera RA: A gene family required for human germ cell development evolved from an ancient meiotic gene conserved in metazoans. Proc natl Acad Sci, USA 98:7414–7419 (2001).
  67. Xu EY, Lee DF, Klebes A, Turek PJ, Kornberg TB, Reijo Pera RA: Human BOULE gene rescues meiotic defects in infertile flies. Hum molec Genet 12:169–175 (2003).
  68. Zhao GQ, Garbers DL: Male germ cell specification and differentiation. Dev Cell 2:537–547 (2002).
  69. Zhong J, Peters AH, Lee K, Braun RE: A double-stranded RNA binding protein required for activation of repressed messages in mammalian germ cells. Nature Genet 22:171–174 (1999).
  70. Zhong J, Peters AH, Kafer K, Braun RE: A highly conserved sequence essential for translational repression of the protamine 1 messenger RNA in murine spermatids. Biol Reprod 64:1784–1789 (2001).

Article / Publication Details

First-Page Preview
Abstract of Paper

Received: September 11, 2003
Accepted: November 14, 2003
Published online: March 30, 2004
Issue release date: 2003

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.