Cytogenetic and Genome Research
Histone H1t is not replaced by H1.1 or H1.2 in pachytene spermatocytes or spermatids of H1t-deficient miceaZentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie I, und bGöttinger Zentrum für Molekulare Biowissenschaften (GZMB), Abteilung Entwicklungsbiologie, Georg-August-Universität Göttingen, Göttingen; cBiozentrum der Universität Würzburg, Lehrstuhl für Zell- und Entwicklungsbiologie, Würzburg (Germany)
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Cytogenet Genome Res 103:307–313 (2003)
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Article / Publication Details
First-Page Preview
Received: September 10, 2003
Accepted: October 20, 2003
Published online: March 30, 2004
Issue release date: 2003
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
Abstract
The linker histone gene H1t is exclusively expressed in the mammalian testis. In former experiments we have shown that H1.1 and H1.2 histone gene expression is significantly enhanced in testis of adult H1t deficient mice. In this report we have quantified the mRNA of different H1 genes in 9-day- and 20-day-old wild type and H1t knock out mice. In addition, we have analysed the distribution of H1.1 and H1.2 protein by immunofluorescent staining in spread male germ cells. The aim of this work was to answer the question whether H1t can be replaced during spermatogenesis by H1.1 or H1.2. In our experiments we could not detect elevated levels of H1.1 or H1.2 in pachytene spermatocytes or haploid cells of H1t deficient testis. Therefore, in these cells, H1t seems not to be replaced by H1.1 or H1.2.
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Article / Publication Details
First-Page Preview
Received: September 10, 2003
Accepted: October 20, 2003
Published online: March 30, 2004
Issue release date: 2003
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
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