Digestion
Original Paper: Bile
Continuous Monitoring of Nitric Oxide Release Induced by Cholecystokinin from the Choledochal Sphincter in Guinea PigsShima Y.a · Mori M.a · Takakura N.a · Tanaka N.a · Yokoi I.b · Kabuto H.b · Yamazato T.caFirst Department of Surgery and bDepartment of Neuroscience, Institute of Molecular and Cellular Medicine, Okayama University Medical School, Okayama, cDepartment of Food and Nutrition, Okayama Women’s College, Kurashiki, Japan
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Article / Publication Details
Published online: February 25, 2000
Issue release date: 2000
Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 1
ISSN: 0012-2823 (Print)
eISSN: 1421-9867 (Online)
For additional information: https://www.karger.com/DIG
Abstract
Background/Aims: Many in vitro studies in the choledochoduodenal junction of the guinea pig have shown that cholecystokinin (CCK) contracts the sphincter of Oddi (SO). This study, using the choledochal sphincter of the guinea pig as the SO, evaluates the hypothesis that effects of CCK on the SO were mediated by nitric oxide (NO). Methods: Spontaneous motility and effects of CCK on the choledochal sphincter were recorded using a constant-perfusion technique, and direct measurement of NO release using a specific NO sensor was performed at the same time. Results: CCK-8 decreased the phasic wave amplitude of the choledochal sphincter, and increased NO release. NG-L-arginine-methyl-ester (L-NAME), an inhibitor of NO synthase, increased the spontaneous motility and converted the CCK-induced inhibitory response into an excitatory response. L-NAME also reduced NO release and abolished the increase of NO that had been caused by CCK-8. These effects were reduced by treatment with L-arginine (L-Arg). L-Arg application enhanced NO release, and recovered the increase of NO by CCK-8. Conclusion: These studies demonstrate that CCK relaxes the choledochal sphincter and this relaxant response is mediated by NO.
© 2000 S. Karger AG, Basel
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Article / Publication Details
Published online: February 25, 2000
Issue release date: 2000
Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 1
ISSN: 0012-2823 (Print)
eISSN: 1421-9867 (Online)
For additional information: https://www.karger.com/DIG
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