Original Paper: Stomach
Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of RatsGunin A.G. · Nikolaev D.V.
Department of Histology, Medical Institute Chuvash State University, Cheboksary, Russia
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Background/Aims: It has been shown that acute or short-term treatments with glucocorticoids lead to a marked decrease in proliferation in the stomach and large intestine. The effects of more prolonged glucocorticoid treatment on cell renewal in these organs are not known. The present work was therefore undertaken to examine the proliferative activity in the stomach and colon during 2 months of glucocorticoid treatment in comparison with shorter treatments. Methods: Rats were treated with either the glucocorticoid triamcinolone acetonide or vehicle for 63, 33 or 3 days. Proliferation was assessed in the glandular epithelium of the fundal part of the stomach and in the epithelium of the colonic crypts using three criteria: the mitotic index; the bromodeoxyuridinelabelling index, and the proliferating cell nuclear antigen-labelling index (percentage of mitotic or labelled cells). Results: Treatment with glucocorticoid for 63 days resulted in a very significant increase in all proliferative parameters tested in the gastric mucosa and the colonic crypts. On the contrary, treatments with glucocorticoid for 3 or 33 days had a marked inhibitory influence on proliferation in these tissues. Conclusion: As opposed to treatments for 3 or 33 days, glucocorticoid treatment for 2 months leads to an increase in the number of cycling cells in the gastric and colonic mucosae.
© 2000 S. Karger AG, Basel
- Berrada, A, Denis MG, Blanchardie P, Chadeneau C, Lustenberger P: Glucocorticoid effects and receptors in two rat colon carcinoma cell lines differing by their tumorigenicity. J Steroid Biochem Mol Biol 1990;37:223–230.
- Braunschweiger PG, Ting HL, Schiffer LM: Correlation between glucocorticoid receptor content and the antiproliferative effect of dexamethasone in experimental solid tumors. Cancer Res 1983;43:4757–4761.
- Cha HH, Cram EJ, Wang EC, Huang AJ, Kasler HG, Firestone GL: Glucocorticoids stimualte p21 gene expression by targeting multiple transcriptional elements within a steroid responsive region of the p21waf1/cip1 promoter in rat hepatoma cells. J Biol Chem 1998;273:1998–2007.
- Denis MG, Chadeneau C, Blanchardie P, Lustenberger P: Biological effects of glucocorticoid hormones on two rat colon adenocarcinoma cell lines. J Steroid Biochem Mol Biol 1992;41:739–745.
- Eastwood GL, Quimby GF, Laferriere JR: Effects of chronic steroid ingestion on gastroduodenal epithelial renewal in the rat. Cell Tissue Kinet 1981;14:405–411.
- Fath RB Jr, Deschner EE, Winawer SJ, Dworkin BM: Degraded carrageenan-induced colitis in CF1 mice. A clinical, histopathological and kinetic analysis. Digestion 1984;29:197–203.
- Gama P, Alvares E: Corticosterone treatment inhibits cell proliferation in the gastric epithelium of suckling rats. J Gastroenterol 1998;33:32–38.
- Hatakeyama S, Suzuki A, Yoshizumi N, Sato M, Nishiya I: Glucocorticoid-induced G1 arrest and the release effect of epidermal growth factor on the human salivary gland adenocarcinoma cell. Cell Biol Int Rep 1991;15:55–65.
- Ichinose M, Tsukada S, Fujimitsu Y, Tatematsu M, Matsubara Y, Yahagi N, Oka M, Suzuki T, Shimizu Y, Yonezawa S, Kageyama T, Miki K, Fukamachi H: Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts. Dev Growth Differ 1997;39:635–642.
Kuwayama H, Matsuo Y, Eastwood GL: Effects of sucralfate, lansoprazole, and cimetidine on the delayed healing by hydrocortisone sodium phosphate of chronic gastric ulcers in the rat. Am J Med 1991;91(suppl):15–19.
- Li J, Johnson TA, Hanson LA, Beer DG: Loss of glucocorticoid-dependent growth inhibition in transformed mouse lung cells. Mol Carcinog 1996;16:213–220.
- Magami Y, Azuma T, Sasaki Z, Inokuchi H, Kawai K: The effect of solfacone on the kinetics of the generative cells and superficial epithelial cells in mouse gastric mucosa. Nippon Yakurigaku Zasshi 1989;94:363–369.
- Sanchez I, Goya L, Vallerga AK, Firestone GL: Glucocorticoids reversibly arrest rat hepatoma cell growth by inducing an early G1 block in cell cycle progression. Cell Growth Differ 1993;4:215–225.
- Schneider A, Grosch G, Stange EF, Ditschuneit H: Influence of triamcinolone and somatostatin on morphometric parameters of cultured intestinal mucosa. In Vitro Cell Dev Biol 1986;22:647–652.
- Tsukada S, Ichinose M, Yahagi N, Matsubara Y, Yonezawa S, Shiokawa K, Furihata C, Miki K, Fukamachi H: Induction of prococious pepsinogen synthesis by glucocorticoids in fetal rat gastric epithelium in organ culture: Importance of mesenchyme for epithelial differentiation. Differentiation 1998;62:239–247.
- Wright NA, Al Dewachi HS, Appleton DR, Watson AJ: The effect of single and of multiple doses of prednisolone tertiary butyl acetate on cell population kinetics in the small bowel mucosa of the rat. Virchows Arch B Cell Pathol 1978;28:339–350.
- Burrin DG, Wester TJ, Davis TA, Fiorotto ML, Chang X: Dexamethasone inhibits small intestinal growth via increased protein catabolism in neonatal pigs. Am J Physiol 1999;276:E269–E277.
- Martinsson T: Ropivacaine inhibits serum-induced proliferation of colon adenocarcinoma cells in vitro. J Pharmacol Exp Ther 1999;288:660–664.
- Berkovitz GD, Carter KM, Migeon CJ, Brown TR: Down-regulation of the glucocorticoid receptor by dexamethasone in cultured human skin fibroblasts: Implications for the regulation of aromatase activity. J Clin Endocrinol Metab 1988;66:1029–1036.
- Sapolsky RM: Stress, glucocorticoids, and damage to the nervous system: The current state of confusion. Stress 1996;1:1–19.
- Schmidt TJ, Meyer AS: Autoregulation of corticosteroid receptors. How, when, where, and why? Receptor 1994;4:229–257.
- Ray D: Molecular mechanisms of glucocorticoid resistance. J Endocrinol 1996;149:1–5.
- Gunin A: Effect of long-term glucocorticoid treatment on oestradiol-induced proliferation in the uterus of ovariectomized rats. J Endocrinol 1998;157:481–488.
- Mikhail G, Sweet L, Mellinger R: Long-term intramuscular administration of triamcinolone acetonide. Arch Dermatol 1977;113:111–114.
- Lypka A, Lazowski J, Szczudlik A, Wasek Z: The depot-form of glucocorticoid decreases cortisol but not ACTH and beta-endorphin levels in human plasma. Horm Metab Res 1983;15:104–106.
- Sawtell RM, Rew DA, Stradling RN, Wilson GD: Pan cycle expression of proliferating cell nuclear antigen in human colorectal and its proliferative correlations. Cytometry 1995;22:190–199.
- Woods AL, Hall PA, Shepherd NA, Hanby AM, Waseem NH, Lane DP, Levison DA: The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. Histopathology 1991;19:21–27.
- Buts JP, De Meyer R, Kolanowski J: Ontogeny of cell proliferation and DNA synthesis in rat colon: Role of glucocorticoids. Am J Physiol 1983;244:G469–G474.
- Castrup HJ: Regeneration of the gastric and intestinal mucosas. Fortschr Med 1979;97:877–880.
Rogatsky I, Trowbridge JM, Garabedian MJ: Glucocorticoid receptor-mediated cell cycle arrest is achieved through distinct cell-specific transcriptional regulatory mechanisms. Mol Cell Biol 1997;17:181–193.
- King KL, Cidlowski JA: Cell cycle regulation and apoptosis. Annu Rev Physiol 1998;60:601–617.
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.