Cytogenetic and Genome Research
Comparative Genomics and Molecular Evolution
Tandem insertions of Alu elementsEl-Sawy M. · Deininger P.Tulane Cancer Center, Department of Environmental Health Sciences, Tulane University Health Sciences Center, New Orleans, LA (USA)
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Article / Publication Details
Received: September 05, 2003
Accepted: November 25, 2003
Published online: November 12, 2004
Issue release date: November 2004
Number of Print Pages: 5
Number of Figures: 2
Number of Tables: 2
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
Abstract
Alu elements are non-autonomous, non-LTR retroposons that represent the most abundant mobile elements in the human genome (1.1 × 106 copies/genome). They preferentially insert adjacent to existing Alu elements. It has been proposed that Alu elements utilize LINE-1 machinery for their retroposition. The LINE-1 endonuclease cleaves at a loose consensus sequence. We have utilized a bioinformatics approach to show the order of insertion of pairs of young (Y) and old (S or J) Alu subfamily members. Our data suggest that the consensus LINE-1 endonuclease cleavage site used for insertion of the old Alu elements can be reused for integration of the younger ones inserting adjacent to them. However, there is also a preference at the 3′ end of Alu into a non-ideal cleavage site that may represent unique properties of the A-tail for integration. Alu elements inserting adjacent to one another may suggest the saturation of the optimal integration sites with existing Alu elements, rather than any innate preference for Alu elements to integrate adjacent to other Alus.
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References
- Batzer MA, Deininger PL, Hellmann-Blumberg U, Jurka J, Labuda D, Rubin CM, Schmid CW, Zietkiewicz E, Zuckerkandl E: Standardized nomenclature for Alu repeats. J Mol Evol 42:3–6 (1996).
-
Cost GJ, Boeke JD: Targeting of human retrotransposon integration is directed by the specificity of the L1 endonuclease for regions of unusual DNA structure. Biochemistry 37:18081–18093 (1998).
- Cost GJ, Golding A, Schlissel MS, Boeke JD: Target DNA chromatinization modulates nicking by L1 endonuclease. Nucl Acids Res 29:573–577 (2001).
- Cost GJ, Feng Q, Jacquier A, Boeke JD: Human L1 element target-primed reverse transcription in vitro. EMBO J 21:5899–5910 (2002).
-
Deininger PL, Batzer MA: Mammalian retroelements. Genome Res 12:1455–1465 (2002).
External Resources
-
Deininger P, Daniels G: The recent evolution of mammalian repetitive DNA elements. Trends Genet 2:76–80 (1986).
External Resources
- Dewannieux M, Esnault C, Heidmann T: LINE-mediated retrotransposition of marked Alu sequences. Nat Genet 35:41–48 (2003).
-
Jurka J: Sequence patterns indicate an enzymatic involvement in integration of mammalian retroposons. Proc Natl Acad Sci USA 94:1872–1877 (1997).
- Kajikawa M, Okada N: LINEs mobilize SINEs in the eel through a shared 3′ sequence. Cell 111:433–444 (2002).
-
Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC et al: Initial sequencing and analysis of the human genome. International Human Genome Sequencing Consortium. Nature 409:860–921 (2001).
- Ostertag EM, Kazazian HH Jr: Biology of L1 retrotransposons. Ann Rev Genet 35:501–538 (2001).
- Rogers JH: The origin and evolution of retroposons. Int Rev Cytol 93:187–279 (1985).
- Roy-Engel AM, Carroll ML, El-Sawy M, Salem A-H, Garber RK, Nguyen SV, Deininger PL, Batzer MA: Non-traditional Alu evolution and primate genomic diversity. J Mol Biol 316:1033–1040 (2002).
- Salem AH, Kilroy GE, Watkins WS, Jorde LB, Batzer MA: Recently integrated Alu elements and human genomic diversity. Mol Biol Evol 20:1349–1361 (2003).
- Shen M, Batzer M, Deininger P: Evolution of the master Alu gene(s). J Mol Evol 33:311–320 (1991).
- Slagel V, Flemington E, Traina-Dorge V, Bradshaw H, Deininger P: Clustering and sub-family relationships of the Alu family in the human genome. Mol Biol Evol 4:19–29 (1987).
- Weiner A, Deininger P, Efstradiatis A: The reverse flow of genetic information: pseudogenes and transposable elements derived from nonviral cellular RNA. Ann Rev Biochem 55:631–661 (1986).
- Xing J, Salem AH, Hedges DJ, Kilroy GE, Watkins WS, Schienman JE, Stewart C-B, Jurka J, Jorde LB, Batzer MA: Comprehensive analysis of two Alu Yd subfamilies. J Mol Evol 57 (suppl 1):S76–89 (2003).
Article / Publication Details
Received: September 05, 2003
Accepted: November 25, 2003
Published online: November 12, 2004
Issue release date: November 2004
Number of Print Pages: 5
Number of Figures: 2
Number of Tables: 2
ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)
For additional information: https://www.karger.com/CGR
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