Journal of Vascular Research

Research Paper

Pathogen Burden, Inflammation, Proliferation and Apoptosis in Human In-Stent Restenosis

Tissue Characteristics Compared to Primary Atherosclerosis

Skowasch D.a · Jabs A.a · Andrié R.a · Dinkelbach S.a · Schiele T.M.b · Wernert N.c · Lüderitz B.a · Bauriedel G.a

Author affiliations

aDepartment of Cardiology, University of Bonn, Bonn; bCardiology Division, University of Munich, Munich, and cInstitute of Pathology, University of Bonn, Bonn, Germany

Keywords: ApoptosisInfectionInflammationProliferationIn-stent restenosisStents

Related Articles for ""
J Vasc Res 2004;41:525–534
https://doi.org/10.1159/000081809

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: July 02, 2004
Accepted: September 10, 2004
Published online: December 03, 2004
Issue release date: November – December

Number of Print Pages: 10
Number of Figures: 4
Number of Tables: 2

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR

Abstract

Pathogenic events leading to in-stent restenosis (ISR) are still incompletely understood. Among others, inflammation, immune reactions, deregulated cell death and growth have been suggested. Therefore, atherectomy probes from 21 patients with symptomatic ISR were analyzed by immunohistochemistry for pathogen burden and compared to primary target lesions from 20 stable angina patients. While cytomegalovirus, herpes simplex virus, Epstein-Barr virus and Helicobacter pylori were not found in ISR, acute and/or persistent chlamydial infection were present in 6/21 of these lesions (29%). Expression of human heat shock protein 60 was found in 8/21 of probes (38%). Indicated by distinct signals of CD68, CD40 and CRP, inflammation was present in 5/21 (24%), 3/21 (14%) and 2/21 (10%) of ISR cases. Cell density of ISR was significantly higher than that of primary lesions (977 ± 315 vs. 431 ± 148 cells/mm2; p < 0.001). There was no replicating cell as shown by Ki67 or PCNA. TUNEL+ cells indicating apoptosis were seen in 6/21 of ISR specimens (29%). Quantitative analysis revealed lower expression levels for each intimal determinant in ISR compared to primary atheroma (all p < 0.05). In summary, human ISR at the time of clinical presentation is characterized by low frequency of pathogen burden and inflammation, but pronounced hypercellularity, low apoptosis and absence of proliferation.

© 2004 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: July 02, 2004
Accepted: September 10, 2004
Published online: December 03, 2004
Issue release date: November – December

Number of Print Pages: 10
Number of Figures: 4
Number of Tables: 2

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR

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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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2004, Vol.41, No. 6

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