Brain, Behavior and Evolution
Original Paper
Myelinogenesis in the Brachial and Lumbosacral Enlargements of the Spinal Cord of the Opossum Monodelphis domesticaLamoureux S.a · Gingras J.b · Cabana T.aaDépartement de sciences biologiques, Université de Montréal, bResearch Institute of the McGill University Health Center, Montréal, Canada
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Brain Behav Evol 2005;65:143–156
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Article / Publication Details
First-Page Preview
Received: March 25, 2004
Accepted: July 12, 2004
Published online: March 07, 2005
Issue release date: March 2005
Number of Print Pages: 14
Number of Figures: 6
Number of Tables: 1
ISSN: 0006-8977 (Print)
eISSN: 1421-9743 (Online)
For additional information: https://www.karger.com/BBE
Abstract
Using immunohistochemistry in light microscopy, the myelin basic protein and proteolipid protein were localized on sections of the spinal cord enlargements of opossums, Monodelphis domestica, to determine the timecourse of myelinogenesis therein and compare it with other events of motor systems development. Additional tissue not processed for immunohistochemistry was prepared for transmission electron microscopy. No immunolabeling for either protein occurred on spinal sections from the newborn opossum, but in electron microscopy occasional fibers surrounded by loose, irregular membranous rings were seen on the outskirts of the ventral horn. Immunolabeling was detected first in the brachial enlargement during the second week, presumably on motoneuronal, vestibular and reticular axons. The areas of the dorsal columns, other spino-encephalic, reticulospinal and propriospinal projections became labeled in the third week, and the area of rubrospinal axons at 4 weeks. In the brachial gray matter, immunolabeling appeared along ventrodorsal and lateromedial gradients from the fourth to seventh weeks. Labeling developed similarly in the white and gray matter of the lumbosacral enlargement, but 3–5 days later than at brachial levels. Labeling intensity in the white and gray matter increased until at least 4 months, but remained light in laminae I–III. Thus, myelinogenesis in the spinal cord enlargements of the opossum is protracted and follows general rostrocaudal, ventrodorsal and lateromedial sequences. It occurs later than synaptogenesis at comparable levels of the cord, but earlier than myelinogenesis in the corresponding ventral and dorsal roots. Spinal myelinogenesis correlates with the development of sensorimotor reflexes, weight support and quadrupedal locomotion.
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Article / Publication Details
First-Page Preview
Received: March 25, 2004
Accepted: July 12, 2004
Published online: March 07, 2005
Issue release date: March 2005
Number of Print Pages: 14
Number of Figures: 6
Number of Tables: 1
ISSN: 0006-8977 (Print)
eISSN: 1421-9743 (Online)
For additional information: https://www.karger.com/BBE
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