Login to MyKarger

New to MyKarger? Click here to sign up.



Login with Facebook

Forgot your password?

Authors, Editors, Reviewers

For Manuscript Submission, Check or Review Login please go to Submission Websites List.

Submission Websites List

Institutional Login
(Shibboleth or Open Athens)

For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.

Research Paper

Adenovirus-Mediated Gene Transfer of Superoxide Dismutase and Catalase Decreases Restenosis after Balloon Angioplasty

Durand E. · Al Haj Zen A. · Addad F. · Brasselet C. · Caligiuri G. · Vinchon F. · Lemarchand P. · Desnos M. · Bruneval P. · Lafont A.

Author affiliations

European Georges Pompidou Hospital, AP-HP, INSERM E00-16, Faculté de Médecine Paris V, Université René Descartes, Paris, France

Related Articles for ""

J Vasc Res 2005;42:255–265

Do you have an account?

Login Information





Contact Information











I have read the Karger Terms and Conditions and agree.



Login Information





Contact Information











I have read the Karger Terms and Conditions and agree.



To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.


Save over 20% compared to the individual article price.
Learn more

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00


Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: December 30, 2004
Accepted: March 19, 2005
Published online: June 03, 2005
Issue release date: May – June

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR

Abstract

Background: Reactive oxygen species (ROS) production increases after injury and potentially contributes to restenosis after angioplasty. We therefore evaluated the effect of adenovirus-mediated gene transfer (Ad) of superoxide dismutase (SOD) and catalase (CAT) on ROS production and restenosis after balloon angioplasty. Methods: O2 and H2O2 production was quantified in cultured cells after incubation with either LPS or CuSO4. Angioplasty and gene transfer were performed in rabbit atherosclerotic iliac arteries. One artery was injected with AdSOD and AdCAT, while the contralateral artery was injected with an adenovirus carrying no transgene, and served as control. Results: ROS production was significantly decreased after adenovirus-mediated gene transfer of SOD and CAT as compared with control. Treated arteries showed less restenosis (32 ± 27 vs. 63 ± 19%, p = 0.003) and less constrictive remodeling (1.2 ± 0.3 vs. 0.9 ± 0.2, p = 0.02) than control arteries. Arteries injected with AdSOD and AdCAT showed better vasoreactivity to acetylcholine (11 ± 4 vs. –1 ± 6%, p < 0.05), lower collagen density (43 ± 16 vs. 53 ± 23%, p = 0.03), and lower inflammatory cell infiltration (22 ± 6 vs. 36 ± 11%, p = 0.04) than control arteries. Conclusions: Our data suggest that adenovirus-mediated gene transfer of SOD and CAT reduced oxidative stress, restenosis, collagen accumulation, and inflammation and improved endothelial function after angioplasty.

© 2005 S. Karger AG, Basel


References

  1. Griendling KK, Fitzgerald GA: Oxidative stress and cardiovascular injury. II. Animal and human studies. Circulation 2003;108:2034–2440.
  2. Azevedo LC, Pedro MA, Souza LC, de Souza HP, Janiszewski M, da Luz PL, Laurindo FR: Oxidative stress as a signaling mechanism of the vascular response to injury: The redox hypothesis of restenosis. Cardiovasc Res 2000;47:436–445.
  3. Pollman MJ, Hall JL, Gibbons GH: Determinants of vascular smooth muscle cell apoptosis after balloon angioplasty injury. Influence of redox state and cell phenotype. Circ Res 1999;84:113–121.
  4. Szocs K, Lassegue B, Sorescu D, Hilenski LL, Valppu L, Couse TL, Wilcox JN, Quinn MT, Lambeth JD, Griendling KK: Upregulation of Nox-based NAD(P)H oxidases in restenosis after carotid injury. Arterioscler Thromb Vasc Biol 2002;22:21–27.
  5. Jacobson GM, Dourron HM, Liu J, Carretero OA, Reddy DJ, Andrzejewski T, Pagano PJ: Novel NAD(P)H oxidase inhibitor suppresses angioplasty-induced superoxide and neointimal hyperplasia of rat carotid artery. Circ Res 2003;92:637–643.
  6. Miller FJ Jr, Gutterman DD, Rios CD, Heistad DD, Davidson BL: Superoxide production in vascular smooth muscle contributes to oxidative stress and impaired relaxation in atherosclerosis. Circ Res 1998;82:1298–1305.
  7. Ruef J, Hu ZY, Yin LY, Wu Y, Hanson SR, Kelly AB, Harker LA, Rao GN, Runge MS, Patterson C: Induction of vascular endothelial growth factor in balloon-injured baboon arteries. A novel role for reactive oxygen species in atherosclerosis. Circ Res 1997;81:24–33.
  8. Liao F, Andalibi A, Qiao JH, Allayee H, Fogelman AM, Lusis AJ: Genetic evidence for a common pathway mediating oxidative stress, inflammatory gene induction, and aortic fatty streak formation in mice. J Clin Invest 1994;94:777–884.
    External Resources
  9. Lafont A, Guzman LA, Whitlow PL, Goormastic M, Cornhill JF, Chisolm GM: Restenosis after experimental angioplasty: Intimal, medial, and adventitial changes associated with constrictive remodeling. Circ Res 1995;76:996–1003.
  10. Lafont A, Durand E, Samuel JL, Besse B, Addad F, Levy BI, Desnos M, Guerot C, Boulanger CM: Endothelial dysfunction and collagen accumulation: Two independent factors for restenosis and constrictive remodeling. Circulation 1999;100:1109–1115.
  11. Lafont AM, Chai YC, Cornhill JF, Whitlow PL, Howe PH, Chisolm GMA, et al: Effect of alpha-tocopherol on restenosis after angioplasty in a model of experimental atherosclerosis. J Clin Invest 1995;95:1018–1025.
  12. Nunes GL, Sgoutas DS, Redden RA, Sigman SR, Gravanis MB, King SB 3rd, Berk BC: Combination of vitamins C and E alters the response to coronary balloon injury in the pig. Arterioscler Thromb Vasc Biol 1995;15:156–165.
  13. Cote G, Tardif JC, Lesperance J, Lambert J, Bourassa M, Bonan R, Gosselin G, Joyal M, Tanguay JF, Nattel S, Gallo R, Crepeau J: Multivitamins and Probucol Study Group. Effect of probucol on vascular remodeling after coronary angioplasty. Circulation 1999;99:30–35.
  14. Griendling KK, Fitzgerald GA: Oxidative stress and cardiovascular injury. I. Mechanisms and in vivo monitoring of ROS. Circulation 2003;108:1912–1916.
  15. Rutanen J, Puhakka H, Yla-Herttuala S: Post-intervention vessel remodeling. Gene Ther 2002;92:1487–1491.
  16. Erzurum SC, Lemarchand P, Rosenfeld MA, Yoo JH, Crystal RG: Protection of human endothelial cells from oxidant injury by adenovirus-mediated transfer of human catalase cDNA. Nucleic Acids Res 1993;21:1607–1612.
  17. Danel C, Erzurum SC, Prayssac P, Eissa NT, Crystal RG, Herve P, Baudet B, Mazmanian M, Lemarchand P: Gene therapy for oxidant injury-related diseases: Adenovirus-mediated transfer of superoxide dismutase and catalase cDNA protects against hyperoxia but not against ischemia-reperfusion lung injury. Hum Gene Ther 1998;9:1487–1496.
  18. Rodriguez-Campos A, Ruiz-Enriquez P, Faraudo S, Badimon L: Mitogen-induced p53 downregulation precedes vascular smooth muscle cell migration from healthy tunica media and proliferation. Arterioscler Thromb Vasc Biol 2001;21:214–219.
  19. Kakuta T, Currier JW, Haudenschild CC, Ryan TJ, Faxon DP: Differences in compensatory vessel enlargement, not intimal formation, account for restenosis after angioplasty in the hypercholesterolemic rabbit model. Circulation 1994;89:2809–2815.
  20. Varenne O, Pislaru S, Gillijns H, Van Pelt N, Gerard RD, Zoldhelyi P, Van de Werf F, Collen D, Janssens SP: Local adenovirus-mediated transfer of human endothelial nitric oxide synthase reduces luminal narrowing after coronary angioplasty in pigs. Circulation 1998;98:919–926.
  21. Strauss BH, Wilson RA, van Houten R, van Suylen RJ, Murphy ES, Escaned J, Verdouw PD, Serruys PW, van der Giessen WJ: Late effects of locally delivered mitomycin C on formation of neointima and on vasomotor response to acetylcholine. Coron Artery Dis 1994;5:633–641.
  22. Ward MR, Kanellakis P, Ramsey D, Funder J, Bobik A: Eplerenone suppresses constrictive remodeling and collagen accumulation after angioplasty in porcine coronary arteries. Circulation 2001;104:467–472.
  23. Boykiw R, Sciore P, Reno C, Marchuk L, Frank CB, Hart DA: Altered levels of extracellular matrix molecule mRNA in healing rabbit ligaments. Matrix Biol 1998;17:371–378.
  24. Braga PC, dal Sasso M, Bovio C, Sgaragli GP: Effect of butylated hydroxyanisole and some of its derivatives on human neutrophil oxidative burst: Chemiluminescence evaluation. Pharmacology 2003;68:9–16.
  25. Durand E, Mallat Z, Addad F, Vilde F, Desnos M, Guerot C, Tedgui A, Lafont A: Time courses of apoptosis and cell proliferation and their relationship to arterial remodeling and restenosis after angioplasty in an atherosclerotic rabbit model. J Am Coll Cardiol 2002;39:1680–1685.
  26. Li PF, Dietz R, von Harsdorf R: Differential effect of hydrogen peroxide and superoxide anion on apoptosis and proliferation of vascular smooth muscle cells. Circulation 1997;96:3602–3609.
  27. Rao GN, Berk BC: Active oxygen species stimulate vascular smooth muscle cell growth and proto-oncogene expression. Circ Res 1992;18:775–794.
  28. Brown MR, Miller FJ Jr, Li WG, Ellingson AN, Mozena JD, Chatterjee P, Engelhardt JF, Zwacka RM, Oberley LW, Spector AA, Weintraub NL: Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells. Circ Res 1999;85:524–533.
  29. Wykretowicz A, Dziarmaga M, Szczpanik A, Guzik P, Wysocki H: Prospective evaluation of hydroperoxide plasma levels and stable nitric oxide end products in patients subjected to angioplasty for coronary artery disease. Int J Cardiol 2003;89:173–178.
  30. Laukkanen MO, Kivela A, Rissanen T, Rutanen J, Karkkainen MK, Leppanen O, Brasen JH, Yla-Herttuala S: Adenovirus-mediated extracellular superoxide dismutase gene therapy reduces neointima formation in balloon-denuded rabbit aorta. Circulation 2002;106:1999–2003.
  31. Six I, Van Belle E, Bordet R, Corseaux D, Callebert J, Dupuis B, Bauters C, Bertrand ME: L-Arginine and L-NAME have no effects on the reendothelialization process after arterial balloon injury. Cardiovasc Res 1999;43:731–738.
  32. Lau AK, Leichtweis SB, Hume P, Mashima R, Hou JY, Chaufour X, Wilkinson B, Hunt NH, Celermajer DS, Stocker R: Probucol promotes functional reendothelialization in balloon-injured rabbit aortas. Circulation 2003;107:2031–2036.
  33. Shimokawa H, Aarhus L, Vanhoutte PM: Porcine coronary arteries with regenerated endothelium have a reduced endothelium-dependent responsiveness to aggregating platelets and serotonin. Circ Res 1987;61:256–270.
  34. Heitzer T, Schlinzig T, Krohn K, Meinertz T, Munzel T: Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease. Circulation 2001;104:2673–2678.
  35. Zanetti M, Sato J, Katusic ZS, O’Brien T: Gene transfer of superoxide dismutase isoforms reverses endothelial dysfunction in diabetic rabbit aorta. Am J Physiol Heart Circ Physiol 2001;280:H2516–H2523.
  36. Gryglewski RJ, Palmer RMJ, Moncada S: Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor. Nature 1986;320:454–456.
  37. Zanetti M, Katusic ZS, O’Brien T: Adenoviral-mediated overexpression of catalase inhibits endothelial cell proliferation. Am J Physiol Heart Circ Physiol 2002;283:H2626–H2626.
  38. Strauss BH, Chisholm RJ, Keeley FW, Gotlieb AI, Logan RA, Armstrong PW: Extracellular matrix remodeling after balloon angioplasty injury in a rabbit model of restenosis. Circ Res 1994;75:650–658.
  39. Nieto N, Friedman SL, Greenwel P, Cederbaum AI: CYP2E1-mediated oxidative stress induces collagen type 1 expression in rat hepatic cells. Hepatology 1999;30:987–996.
  40. Kinugawa S, Tsutsui H, Hayashidani S, Ide T, Suematsu N, Satoh S, Utsumi H, Takeshita A: Treatment with dimethylthiourea prevents left ventricular remodeling and failure after experimental myocardial infarction in mice: Role of oxidative stress. Circ Res 2000;87:392–398.
  41. Mattana J, Margiloff L, Chaplia L: Oxidation of extracellular matrix modulates susceptibility to degradation by the mesangial matrix metalloproteinase-2. Free Radic Biol Med 1999;27:315–321.
  42. Yamasaki K, Asai T, Shimizu M, Aoki M, Hashiya N, Sakonjo H, Makino H, Kaneda Y, Ogihara T, Morishita R: Inhibition of NFkappaB activation using cis-element ‘decoy’ of NFkappaB binding site reduces neointimal formation in porcine balloon-injured coronary artery model. Gene Ther 2003;10:356–364.
  43. Wasserman MA, Sundell CL, Kunsch C, Edwards D, Meng CQ, Medford RM: Chemistry and pharmacology of vascular protectants: A novel approach to the treatment of atherosclerosis and coronary artery disease. Am J Cardiol 2003;91:34A–40A.
    External Resources
  44. Newman KD, Dunn PF, Owens JW, Schulick AH, Virmani R, Sukhova G, Libby P, Dichek DA: Adenovirus-mediated gene transfer into normal rabbit arteries results in prolonged vascular cell activation, inflammation, and neointimal hyperplasia. J Clin Invest 1995;96:2955–2965.
  45. Lafont A, Durand E, Vilde F, Moreyra C, Drechsel S, Mirshahi SS, Mirshahi M, Guerot C, Lemarchand P: Thrombus generation after adenovirus-mediated gene transfer into atherosclerotic arteries. Hum Gene Ther 1998;9:2795–2800.

Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: December 30, 2004
Accepted: March 19, 2005
Published online: June 03, 2005
Issue release date: May – June

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.