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Original Article

The canine sarcoglycan delta gene: BAC clone contig assembly, chromosome assignment and interrogation as a candidate gene for dilated cardiomyopathy in Dobermann dogs

Stabej P.a · Leegwater P.A.J.a · Imholz S.a · Versteeg S.A.a · Zijlstra C.b · Stokhof A.A.a · Domanjko-Petriè A.d · van Oost B.A.c

Author affiliations

Departments of aClinical Sciences of Companion Animals, bBiochemistry and Cell Biology and cAnimals, Science and Society, Faculty of Veterinary Medicine, Utrecht University, Utrecht (The Netherlands); dVeterinary Faculty, Clinic for Surgery and Small Animal Medicine, University of Ljubljana, Ljubljana (Slovenia)

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Cytogenet Genome Res 111:140–146 (2005)

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Published online: August 18, 2005
Issue release date: August 2005

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 3

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Dilated cardiomyopathy (DCM) is a common disease of the myocardium recognized in human, dog and experimental animals. Genetic factors are responsible for a large proportion of cases in humans, and 17 genes with DCM causing mutations have been identified. The genetic origin of DCM in the Dobermann dogs has been suggested, but no disease genes have been identified to date. In this paper, we describe the characterization and evaluation of the canine sarcoglycan delta (SGCD), a gene implicated in DCM in human and hamster. Bacterial artificial chromosomes (BACs) containing the canine SGCD gene were isolated with probes for exon 3 and exons 4–8 and were characterized by Southern blot analysis. BAC end sequences were obtained for four BACs. Three of the BACs overlapped and could be ordered relative to each other and the end sequences of all four BACs could be anchored on the preliminary assembly of the dog genome sequence (www. ensembl.org). One of the BACs of the partial contig was localized by fluorescent in situ hybridization to canine chromosome 4q22, in agreement with the dog genome sequence. Two highly informative polymorphic microsatellite markers in intron 7 of the SGCD gene were identified. In 25 DCM-affected and 13 non DCM-affected dogs seven different haplotypes could be distinguished. However, no association between any of the SGCD variants and the disease locus was apparent.    

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Published online: August 18, 2005
Issue release date: August 2005

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 3

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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