Antigenicity and Immunocrossreactivity of Orange Tree Pollen and Orange Fruit Allergenic ExtractsIrañeta S.G.a · Seoane M.A.a · Laucella S.A.c · Apicella C.b · Alonso A.a · Duschak V.G.c
aCentro de Alergia, Hospital de Clínicas, bIDEHU, Facultad de Farmacia y Bioquímica, UBA, cInstituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’, ANLIS, Ministerio de Salud y Ambiente, Buenos Aires, Argentina
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Article / Publication Details
Background: It is important to study the crossreactivity between orange tree pollen (OTPE) and orange fruit (OFE) due to the high incidence of pollen/food-related allergies worldwide. The aim of the present study was to determine the antigenic relationship between OTPE and OFE. Methods: OTPE and OFErabbit antisera as well as sera from patients allergic to OTPE and OFE were comparatively applied in IgG- and/or IgE-specific ELISA inhibition, crossover or inhibition immunoblotting assays using OTPE and OFE allergenic extracts as solid phase. Periodate and proteinase K treatments were used for carbohydrate and protein depletion, respectively. Results: The antigenicity of OTPE and the presence of common structures between OTPE and OFE extracts were demonstrated by rabbit IgG-specific ELISA inhibition and crossover immunoblotting assays. A 30-kDa protein, common target of the IgE response on OTPE, OFE and mandarin extract, but absent in lemon extract, was identified by ELISA and immunoblot inhibition assays in patients suffering from primary sensitization to OTPE in the context of occupational exposure. Moreover, biochemical treatments showed that antigenic epitopes on the 30-kDa protein contain polypeptidic but no carbohydrate moieties. Conclusions: The antigenicity of OTPE, the presence of common antigenic determinants between pollen and citrus fruits and an IgE-specific crossreactive protein band of 30 kDa sharing carbohydrate-free epitopes were described. After isolation and purification, this common antigen might be useful for allergen immunotherapy in pollen/fruit-related allergic patients.
© 2005 S. Karger AG, Basel
- Woolcock AJ: Inhaled drugs in prevention of asthma. Am J Respir Dis1977;115:191–194.
- Pham NH, Baldo BA, Bass DJ: Cypress pollen allergy. Identification of pollen allergens and cross-reactivity between divergent species. Clin Exp Allergy1994;24:558–565.
- Suphioglu C: What are the important allergens in grass pollen that are linked to human allergic disease. Clin Exp Allergy 2000;30:1335–1341.
- Pauli G: Evolution in the understanding of cross-reactivities of respiratory allergens: The role of recombinant allergens. Int Arch Allergy Immunol 2000;123:183–195.
- Kazemi-Shirazi L, Pauli G, Purohit A, Spitzauer S, Froschl R, Hoffmann-Sommergruber K, Breiteneder H, Scheiner O, Kraft D, Valenta R: Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for many forms of plant food allergy. J Allergy Clin Immunol 2000;105:116–125.
- Caballero T, Martín-Esteban M: Association between pollen hypersensitivity and edible vegetable allergy: A review. Invest Allergol Clin Immunol 1998;8:6–16.
- Ortolani C, Ispano M, Pastorello E, Bigi A, Ansalomi R: The oral allergy syndrome. Ann Allergy 1988;61:47–52.
- Vieths S, Scheurer S, Ballmer-Weber B: Current understanding of cross-reactivity of food allergens and pollen. Ann NY Acad Sci 2002;964:47–68.
- Wellhausen A, Schoning B, Pettersen A, Vieths S: IgE binding to a new cross-reactive structure: A 35 kDa in birch pollen, exotic fruit and other plant foods. Z Ernahrungswiss 1996;35:348–355.
- Karamloo FA, Wangorsch A, Kasahara H, Davin L, Haustein D, Lewis NG, Vieths S: Phenylcoumaran benzylic ether and isoflavonoid reductases are a new class of cross-reactive allergens in birch pollen, fruits and vegetables. Eur J Biochem 2001;268:5310–5320.
- Halmepuro L, Vuontela K, Kalimo K, Bjorksten F: Crossreactivity and IgE antibodies with allergens in birch pollen fruits and vegetables. Int Arch Appl Immunol 1984;74:235–240.
- Alonso A, Seoane MA, Irañeta S: Induction of anti-orange antibodies in rabbits. Allergol Immunopathol 1989;17:307–311.
- Alonso A, Seoane MA, Irañeta S: A citrus fruit-exclusion diet in sensitive patients and its influence on specific antibodies. J Invest Allergol Clin Immunol 1994;4:146–148.
Frugoni C: Preparación de extractos alergénicos; in Hansen K, Wemer M (eds): Alergia Clínica. Barcelona, Salvat, 1970, pp 508–586.
Irañeta SG, Duschak VG, Rodriguez SM, Seoane MA, Albonico JF, Alonso A: Proteinase and gelatinolytic activities of house dust mite and cockroach extracts. J Invest Allergol Clin Immunol 1999;4:235–240.
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–254.
- Owhashi M, Horii Y, Ishii A: Eosinophil chemotactic factor in schistosome eggs: A comparative study of eosinophil chemotactic factors in the eggs of Schistosoma japonicum and S. mansoni in vitro. Am J Trop Med Hyg 1983;32:359–366.
- Duschak VG, Riarte A, Segura EL, Laucella SA: Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease. Immunol Lett 2001;78:135–142.
- Ouchterloni O: Diffusion-in-gel methods for immunological analysis. Prog Allergy 1958;5:1–16.
- Boyden SV: The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med 1951;93:107–112.
Aas K, Belin L: Standardization of diagnostic work in allergy. Acta Allergologica 1972;27:439–468.
- Ceska M, Eriksson R, Varga JM: Radioallergosorbent assay of allergens. J Allergy Clin Immunol 1972;49:1–9.
- Johansson SGO, Bennich H, Berg T: In vitro diagnostic of atopy allergy. Quantitative estimation of circulating IgE antibodies by the radio allergosorbent test. Int Arch Allergy Appl Immunol 1971;41:443–451.
- De Filippi I, Yman L, Schoder H: Clinical accuracy of updated version of the Phadebas RAST test. Ann Allergy 1981,46:249–255.
- Sridahara S, Singh BP, Kumar L, Verma J, Gaur SN, Gangal SV: Antigenic and allergenic relationships among grass pollens in India. Ann Allergy Asthma Immunol 1995;75:73–79.
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970;27:680–685.
- Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350–4354.
- McGadey A: A tetrazolium method for non-specific alkaline phosphatase. Histochimie 1970;23:180–184.
Chakraborty P, Gupta-Bhatacharya S, Chanda S: Comparative aerobiology, allergenicity and biochemistry of three common palm pollen from Calcuta, India. Aerobiologia1996;12:47–50.
- Mohapatra SS: Is cross-reactivity a real or an imaginary concept? J Allergy Clin Immunol 1997;99:724.
- Wadee AA, Boting LA, Rabson AR: Fruit allergy: Demonstration of IgE antibodies to a 30 kDa protein present in several fruits. J Allergy Clin Immunol 1990;85:801–807.
Lavaud F, Prevost A, Cossart C, Guerin L, Bernard J, Kochman S: Allergens, IgE, mediators, inflammatory mechanisms. Allergy to latex, avocado pear, and banana: Evidence for a 30 kDa antigen in immunoblotting. J Allergy Clin Immunol 1994;95:558–563.
- King TP: Immunochemical properties of some atopic allergens. J Allergy Clin Immunol 1979;64:159–163.
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.