Circulating Oxidized Low-Density Lipoprotein and Paraoxonase Activity in PreeclampsiaUzun H.a · Benian A.b · Madazlı R.b · Topçuoğlu M.A.c · Aydın S.a · Albayrak M.b
Departments of aBiochemistry, and bObstetrics and Gynecology, Istanbul University, Cerrahpaşa Medical Faculty, Istanbul, and cDepartment of Obstetrics and Gynecology, Abant İzzet Baysal University, Medical Faculty, Abant, Turkey
Do you have an account?
- Rent for 48h to view
- Buy Cloud Access for unlimited viewing via different devices
- Synchronizing in the ReadCube Cloud
- Printing and saving restrictions apply
Rental: USD 8.50
Cloud: USD 20.00
Article / Publication Details
Preeclampsia is one of the most frequent complications of pregnancy, however, little is known about its etiology. The objective of this study was to investigate the association of oxidized low-density lipoprotein (oxLDL) and paraoxonase (PON1) activity in women with either preeclampsia or normotensive (NT) pregnancy. The study groups included 41 pregnant women with preeclampsia and 33 normotensive pregnant women. In all patients maternal serum total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TGs) were measured using enzymatic methods. Serum PON1 activities and malondialdehyde (MDA) concentrations were measured by spectrophotometric methods, and oxLDL was measured by enzyme-linked immunoassay (ELISA). Serum concentrations of lipid parameters (TC, LDL, VLDL, and TGs) were significantly higher in preeclampsia compared with NT controls (p < 0.001, p < 0.05, p < 0.05, and p < 0.001, respectively). Serum concentrations of MDA and oxLDL were significantly higher, while PON1 activity was significantly lower in preeclampsia compared with NT controls (p < 0.001, p < 0.001, and p < 0.001, respectively). A positive correlation was detected between oxLDL and MDA (r = 0.876), and a negative correlation was detected between both MDA and oxLDL and PON1 (r = –0.837 and r = –0.759, respectively). Our data demonstrate that preeclampsia is associated with increased oxLDL and decreased PON1 activity. Elevated oxidative stress, oxLDL, dyslipidemia and decreased PON1 activities may cause vascular endothelial damage and contribute to the pathophysiology of preeclampsia.
© 2005 S. Karger AG, Basel
- Roberts JM, Cooper DW: Pathogenesis and genetics of pre-eclampsia. Lancet 2001;357:53–56.
- Redman CW, Sargent IL: The pathogenesis of pre-eclampsia. Gynecol Obstet Fertil 2001;29:518–522.
- Hubel CA: Oxidative stress in the pathogenesis of preeclampsia. Proc Soc Exp Biol Med 1999;222:222–235.
- Orhan H, Önderoglu L, Yücel A, Sahin G: Circulating biomarkers of oxidative stress in complicated pregnancies. Arch Gynecol Obstet 2003;267:189–195.
- Kumar CA, Das UN: Oxidant stress in preeclampsia and eesential hypertension. J Assoc Physicians India 2002;50:1372–1375.
Gurer OH, Özgüneş H, Beksaç MS: Correlation between plasma malondialdehyde and ceruloplasmin activity values in preeclamptic pregnancies. Clin Biochem 2000;34:505–506.
- Kumar CA, Das UN: Lipid peroxides, anti-oxidants and nitric oxide in patients with pre-eclampsia and essential hypertension. Med Sci Monit 2000;6:901–907.
- Steinberg D: Oxidative modification of LDL and atherogenesis. Circulation 1997;95:1062–1071.
- Li D, Mehta JL: 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors protect against oxidized low-density lipoprotein induced endothelial dysfunction. Endothelium 2003;10:17–21.
- Bonnefont-Rousselot D, Therond P, Beaudeux JL, Peynet J, Legrand A, Delattre J: High density lipoproteins (HDL) and oxidative hypothesis of atherosclerosis. Clin Chem Lab Med 1999;37:938–948.
- Roberts JM, Lain KY: Recent insights into the pathogenesis of pre-eclampsia. Placenta 2002;23:359–372.
- Mackness Ml, Mackness B, Durrington PN, Connelly PW, Hegele RA: Paraoxonase: biochemistry, genetics and relationship to p1asma lipoproteins. Curr Opin Lipidol 1996;7:69–76.
- Masse J, Giguere Y, Kharfi A, Girouard J, Forest JC: Pathophysiology and maternal biologic markers of preeclampsia. Endocrine 2002;19:113–125.
American College of Obstetricians and Gynecologists: Hypertension in pregnancy. ACOG Tech Bull 1996;219:1–8.
- Buege JA, Aust SD: Microsomal lipid peroxidation. Methods Enzymol 1978;52:302–310.
- Hasselwander O, Savage DA, McMaster D, Loughrey CM, McNamee PT, Middleton D, Nicholls DP, Maxwell AP, Young IS: Paraoxonase polymorphisms are not associated with cardiovascular risk in renal transplants recipients. Kidney Int 1999;56:289–298.
- Saha N, Roy AC, Teo SH, Tay JS, Ratnam SS: Influence of serum paraoxonase polymorphism on serum lipids and apolipoproteins. Clin Genet 1991;40:277–282.
- Ness RB, Roberts JM: Heterogenous causes constituting the single syndrome of pre-eclampsia, a hypothesis and in implications. Am J Obstet Gynecol 1996;175:1365–1370.
- Madazlı R, Benian A, Gümüştaş K, Uzun H, Ocak V, Aksu F: Lipid peroxidation and antioxidant in preeclampsia. Eur J Obstet Gynecol Reprod Biol 1999;85:205–208.
- McCord JM: The evolution of free radicals and oxidative stress. Am J Med 2000;108:652–659.
- Mackness MI, Mackness B, Durrington PN: Paraoxonase and coronary heart disease. Atheroscler Suppl 2002;3:49–55.
- Mackness B, Durrington PN, Mackness MI: Polymorphisms of paraoxonase genes and low-density lipoprotein lipid peroxidation. Lancet 1999;353:468–469.
- Mackness MI, Arrol S, Mackness B, Durrington PN: Alloenzymes of paraoxonase and effectiveness of high-density lipoproteins in protecting low-density lipoprotein against lipid peroxidation. Lancet 1997;349:851–852.
Roberts JM, Taylor RN, Musci TJ, Rodgers GM, Habel CA, McLaughlin MK: Preeclampsia: an endothelial cell disorder. Am J Obstet Gynecol 1990;163:1365–1366.
- McCarthy AL, Woolfson RG, Raju SK, Poston L: Abnormal endothelial cell function of resistance arteries from women with preeclampsia. Am J Obstet Gynecol 1993;168:1323–1330.
- Uddin M, Yang H, Shi M, Polley-Mandal M, Guo Z: Elevation of oxidative stress in the aorta of genetically hypertensive mice. Mech Ageing Dev 2003;124:811–817.
- Somova LI, Nadar A, Gregory M, Khan N: Antioxidant status of the hypertrophic heart of Dahl hypertensive rat as a model for evaluation of antioxidant. Methods Find Exp Clin Pharmacol 2001;23:5–12.
- Williams MA, Woelk GB, King IB, Jenkins L, Mahomed K: Plasma carotenoids, retinol, tocopherols and lipoproteins in preeclamptic and normotensive pregnant Zimbabwean woman. Am J Hypertens 2003;16:665–672.
- Ware-Jauregui S, Sanchez SE, Zhang C, Laraburre G, King IB, Williams MA: Plasma lipid concentrations in pre-eclampsia and normotensive Peruvian women. Int J Gynecol Obstet 1999;67:147–155.
Gratacos E, Casals E, Gömez O, Llurba E, Mercader I, Cararach V, Cabero L: Increased susceptibility to low density lipoprotein oxidation in women a history of pre-eclampsia. Int J Obstet Gynecol 2003;110:400–404.
- Branch DW, Mitchell MD, Miller E, Palinski W, Witztum JL: Preeclampsia and serum antibodies to oxidised low-density lipoprotein. Lancet 1994;343:645–646.
- Sağol S, Özkınay E, Özşener S: Impaired antioxidant activity in women with pre-eclampsia. Int J Gynecol Obstet 1999;64:121–127.
- Mertens A, Holvoet P: Oxidized LDL and HDL: antagonists in atherothrombosis. FASEB J 2001;15:2073–2084.
- Durrington PN, Mackness B, Mackness MI: Paraoxanase and atherosclerosis. Atherosclor Thromb Vasc Biol 2001;21:473–480.
- Jarvik GP, Tsai NT, McKinstry LA, Wani R, Brophy VH, Richter RJ, Schellenberg GD, Heagerty PJ, Hatsukami TS, Furlong CE: Vitamin C and E intake associated with increased paraoxonase activity. Arterioscler Thromb Vasc Biol 2002;22:1329–1333.
- Kumru S, Aydin S, Gursu MF, Ozcan Z: Changes of serum paraoxonase (an HDL-cholesterol-associated lipophilic antioxidant) and arylesterase activities in severe preeclamptic women. Eur J Obstet Gynecol Reprod Biol 2004;114:177–181.
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.