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Original Paper

Dendritic Cells in Atopic Dermatitis: Expression of FcεRI on Two Distinct Inflammation-Associated Subsets

Stary G. · Bangert C. · Stingl G. · Kopp T.

Author affiliations

Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, Austria

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Int Arch Allergy Immunol 2005;138:278–290

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 09, 2005
Accepted: July 07, 2005
Published online: November 18, 2005
Issue release date: December 2005

Number of Print Pages: 13
Number of Figures: 6
Number of Tables: 4

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: Dendritic cells (DCs) represent a major portion within the infiltrate of atopic dermatitis (AD) lesions. As antigen-presenting cells they have the ability to regulate both the quantity and quality of T-cell responses and, thus, are likely to play a key role in the pathogenesis of T-cell-dominated skin diseases such as AD. Thus we sought to identify the DC repertoire occurring in AD patients. Methods: For this purpose, we phenotypically analyzed various defined DC subsets of AD patients and healthy controls in skin biopsies and peripheral blood by immunofluorescence staining. Results: In AD lesions, two inflammation-associated DC subsets with varying expression of costimulatory molecules occurred besides epidermal Langerhans cells (LCs) and dermal myeloid DCs (dmDCs) indigenously residing in normal skin: (1) CD1a+/CD1c+/FcεRI+/IgE+/CD207– myeloid DCs (mDCs) in the epidermis and dermis and (2) CD123+/BDCA-2+/CD45RA+/CD68+ plasmacytoid DCs (pDCs) in the dermis. In the peripheral blood of the patients, these cells exhibited an immature phenotype. Interestingly, we found FcεRI and cell-bound IgE to be expressed not only on myeloid, but also on plasmacytoid DCs from both the skin and peripheral blood of AD patients. Conclusions: It is tempting to speculate that the disease-regulating role of inflammatory DCs in AD is influenced by both FcεRI occupancy and their degree of maturity.

© 2005 S. Karger AG, Basel


References

  1. Schultz Larsen F: Atopic dermatitis: a genetic-epidemiologic study in a population-based twin sample. J Am Acad Dermatol 1993;28:719–723.
  2. Schultz Larsen F, Diepgen T, Svensson A: The occurrence of atopic dermatitis in north Europe: an international questionnaire study. J Am Acad Dermatol 1996;34:760–764.
  3. Banchereau J, Steinman RM: Dendritic cells and the control of the immunity. Nature 1998;392:245–252.
  4. Soumelis V, Reche PA, Kanzler H, Yuan W, Edward G, Homey B, Gilliet M, Ho S, Antonenko S, Lauerma A, Smith K, Gorman D, Zurawski S, Abrams J, Menon S, McClanahan T, de Waal-Malefyt Rd R, Bazan F, Kastelein RA, Liu YJ: Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol 2002;3:673–680.
  5. Meunier L, Gonzalez-Ramos A, Cooper KD: Heterogeneous populations of class II MHC+ cells in human dermal cell suspensions. Identification of a small subset responsible for potent dermal antigen-presenting cell activity with features analogous to Langerhans cells. J Immunol 1993;151:4067–4080.
  6. Wollenberg A, Kraft S, Hanau D, Bieber T: Immunomorphological and ultrastructural characterization of Langerhans cells and a novel, inflammatory dendritic epidermal cell (IDEC) population in lesional skin of atopic eczema. J Invest Dermatol 1996;106:446–453.
  7. Zou W, Borvak J, Wei S, Isaeva T, Curiel DT, Curiel TJ: Reciprocal regulation of plasmacytoid dendritic cells and monocytes during viral infection. Eur J Immunol 2001;31:3833–3839.
  8. Zou W, Machelon V, Coulomb-L’Hermin A, Borvak J, Nome F, Isaeva T, Wei S, Krzysiek R, Durand-Gasselin I, Gordon A, Pustilnik T, Curiel DT, Galanaud P, Capron F, Emilie D, Curiel TJ: Stromal-derived factor-1 in human tumors recruits and alters the function of plasmacytoid precursor dendritic cells. Nat Med 2001;7:1339–1346.
  9. Pashenkov M, Teleshova N, Kouwenhoven M, Smirnova T, Jin YP, Kostulas V, Huang YM, Pinegin B, Boiko A, Link H: Recruitment of dendritic cells to the cerebrospinal fluid in bacterial neuroinfections. J Neuroimmunol 2002;122:106–116.
  10. Farkas L, Beiske K, Lund-Johansen F, Brandtzaeg P, Jahnsen FL: Plasmacytoid dendritic cells (natural interferon-alpha/beta-producing cells) accumulate in cutaneous lupus erythematosus lesions. Am J Pathol 2001;159:237–243.
  11. Jahnsen FL, Lund-Johansen F, Dunne JF, Farkas L, Haye R, Brandtzaeg P: Experimentally induced recruitment of plasmacytoid (CD123high) dendritic cells in human nasal allergy. J Immunol 2000;165:4062–4068.
  12. Wollenberg A, Wagner M, Gunther S, Towarowski A, Tuma E, Moderer M, Rothenfusser S, Wetzel S, Endres S, Hartmann G: Plasmacytoid dendritic cells: a new cutaneous dendritic cell subset with distinct role in inflammatory skin diseases. J Invest Dermatol 2002;119:1096–1102.
  13. Bangert C, Friedl J, Stary G, Stingl G, Kopp T: Immunopathologic features of allergic contact dermatitis in humans: participation of plasmacytoid dendritic cells in the pathogenesis of the disease? J Invest Dermatol 2003;121:1409–1418.
  14. Cella M, Sallusto F, Lanzavecchia A: Origin, maturation and antigen presenting function of dendritic cells. Curr Opin Immunol 1997;9:10–16.
  15. Fonteneau JF, Gilliet M, Larsson M, Dasilva I, Munz C, Liu YJ, Bhardwaj N: Activation of influenza virus-specific CD4+ and CD8+ T cells: a new role for plasmacytoid dendritic cells in adaptive immunity. Blood 2003;101:3520–3526.
  16. Hanifin JM, Rajka G: Diagnostic features of atopic dermatitis. Acta Derm Venereol Suppl (Stockh) 1980;92:44–47.
  17. Ishizaka T, Ishizaka K: Mechanisms of passive sensitization. IV. Dissociation of IgE molecules from basophil receptors at acid pH. J Immunol 1974;112:1078–1084.
  18. Gilliet M, Conrad C, Geiges M, Cozzio A, Thurlimann W, Burg G, Nestle FO, Dummer R: Psoriasis triggered by toll-like receptor 7 agonist imiquimod in the presence of dermal plasmacytoid dendritic cell precursors. Arch Dermatol 2004;140:1490–1495.
  19. Simon A, Fah J, Haller O, Staeheli P: Interferon-regulated Mx genes are not responsive to interleukin-1, tumor necrosis factor, and other cytokines. J Virol 1991;65:968–971.
  20. Wang B, Rieger A, Kilgus O, Ochiai K, Maurer D, Födinger D, Kinet JP, Stingl G: Epidermal Langerhans cells from normal human skin bind monomeric IgE via Fc epsi RI. J Exp Med 1992;175:1353–1365.
  21. Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J: BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 2000;165:6037–6046.
  22. Pruzansky JJ, Grammer LC, Patterson R, Roberts M: Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release. J Immunol 1983;131:1949–1953.
  23. Fithian E, Kung P, Goldstein G, Rubenfeld M, Fenoglio C, Edelson R: Reactivity of Langerhans cells with hybridoma antibody. Proc Natl Acad Sci USA 1981;78:2541–2544.
  24. Bauer J, Bahmer FA, Worl J, Neuhuber W, Schuler G, Fartasch M: A strikingly constant ratio exists between Langerhans cells and other epidermal cells in human skin. A stereologic study using the optical disector method and the confocal laser scanning microscope. J Invest Dermatol 2001;116:313–318.
  25. Ghannadan M, Hauswirth AW, Schernthaner GH, Muller MR, Klepetko W, Schatzl G, Sperr WR, Buhring HJ, Valent P: Detection of novel CD antigens on the surface of human mast cells and basophils. Int Arch Allergy Immunol 2002;127:299–307.
  26. Maurer D, Fiebiger S, Ebner C, Reininger B, Fischer GF, Wichlas S, Jouvin MH, Schmitt-Egenolf M, Kraft D, Kinet JP, Stingl G: Peripheral blood dendritic cells express Fc epsi RI as a complex composed of Fc epsi RI alpha- and Fc epsi RI gamma-chains and can use this receptor for IgE-mediated allergen presentation. J Immunol 1996;157:607–616.
  27. Fuhlbrigge RC, King SL, Dimitroff CJ, Kupper TS, Sackstein R: Direct real-time observation of E- and P-selectin-mediated rolling on cutaneous lymphocyte-associated antigen immobilized on Western blots. J Immunol 2002;168:5645–5651.
  28. Merad M, Hoffmann P, Ranheim E, Slaymaker S, Manz MG, Lira SA, Charo I, Cook DN, Weissman IL, Strober S, Engleman EG: Depletion of host Langerhans cells before transplantation of donor alloreactive T cells prevents skin graft-versus-host disease. Nat Med 2004;10:510–517.
  29. Strunk D, Egger C, Leitner G, Hanau D, Stingl G: A skin homing molecule defines the Langerhans cell progenitor in human peripheral blood. J Exp Med 1997;185:1131–1136.
  30. Robert C, Fuhlbrigge RC, Kieffer JD, Ayehunie S, Hynes RO, Cheng G, Grabbe S, von Andrian UH, Kupper TS: Interaction of dendritic cells with skin endothelium: A new perspective on immunosurveillance. J Exp Med 1999;189:627–636.
  31. Charbonnier AS, Kohrgruber N, Kriehuber E, Stingl G, Rot A, Maurer D: Macrophage inflammatory protein 3alpha is involved in the constitutive trafficking of epidermal Langerhans cells. J Exp Med 1999;190:1755–1768.
  32. Larregina AT, Morelli AE, Spencer LA, Logar AJ, Watkins SC, Thomson AW, Falo LD Jr: Dermal-resident CD14± cells differentiate into Langerhans cells. Nat Immunol 2001;2:1151–1158.
  33. Valladeau J, Duvert-Frances V, Pin JJ, Dezutter-Dambuyant C, Vincent C, Massacrier C, Vincent J, Yoneda K, Banchereau J, Caux C, Davoust J, Saeland S: The monoclonal antibody DCGM4 recognizes langerin, a protein specific of Langerhans cells, and is rapidly internalized from the cell surface. Eur J Immunol 1999;29:2695–2704.
  34. Teunissen MB, Wormmeester J, Krieg SR, Peters PJ, Vogels IM, Kapsenberg ML, Bos JD: Human epidermal Langerhans cells undergo profound morphologic and phenotypical changes during in vitro culture. J Invest Dermatol 1990;94:166–173.
  35. Wollenberg A, Haberstok J, Teichmann B, Wen SP, Bieber T: Demonstration of the low-affinity IgE receptor Fc epsiRII/CD23 in psoriatic epidermis: inflammatory dendritic epidermal cells (IDEC) but not Langerhans cells are the relevant CD1a-positive cell population. Arch Dermatol Res 1998;290:517–521.
  36. Uchida Y, Kurasawa K, Nakajima H, Nakagawa N, Tanabe E, Sueishi M, Saito Y, Iwamoto I: Increase of dendritic cells of type 2 (DC2) by altered response to IL-4 in atopic patients. J Allergy Clin Immunol 2001;108:1005–1011.
  37. Matsuda H, Suda T, Hashizume H, Yokomura K, Asada K, Suzuki K, Chida K, Nakamura H: Alteration of balance between myeloid dendritic cells and plasmacytoid dendritic cells in peripheral blood of patients with asthma. Am J Respir Crit Care Med 2002;166:1050–1054.
  38. Reider N, Reider D, Ebner S, Holzmann S, Herold M, Fritsch P, Romani N: Dendritic cells contribute to the development of atopy by an insufficiency in IL-12 production. J Allergy Clin Immunol 2002;109:89–95.
  39. Kohrgruber N, Halanek N, Groger M, Winter D, Rappersberger K, Schmitt-Egenolf M, Stingl G, Maurer D: Survival, maturation, and function of CD11c– and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines. J Immunol 1999;163:3250–3259.
  40. Rissoan MC, Soumelis V, Kadowaki N, Grouard G, Briere F, de Waal Malefyt R, Liu YJ: Reciprocal control of T helper cell and dendritic cell differentiation. Science 1999;283:1183–1186.
  41. Siegal FP, Kadowaki N, Shodell M, Fitzgerald-Bocarsly PA, Shah K, Ho S, Antonenko S, Liu YJ: The nature of the principal type 1 interferon-producing cells in human blood. Science 1999;284:1835–1837.
  42. Cella M, Facchetti F, Lanzavecchia A, Colonna M: Plasmacytoid dendritic cells activated by influenza virus and CD40L drive a potent TH1 polarization. Nat Immunol 2000;1:305–310.
  43. Grewe M, Bruijnzeel-Koomen CA, Schopf E, Thepen T, Langeveld-Wildschut AG, Ruzicka T, Krutmann J: A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis. Immunol Today 1998;19:359–361.
  44. Hashizume H, Horibe T, Yagi H, Seo N, Takigawa M: Compartmental imbalance and aberrant immune function of blood CD123+ (plasmacytoid) and CD11c+ (myeloid) dendritic cells in atopic dermatitis. J Immunol 2005:174:2396–2403.
  45. Maurer D, Ebner C, Reininger B, Fiebiger E, Kraft D, Kinet JP, Stingl G: The high affinity IgE receptor (Fc epsi RI) mediates IgE-dependent allergen presentation. J Immunol 1995;154:6285–6290.
  46. Foster B, Metcalfe DD, Prussin C: Human dendritic cell 1 and dendritic cell 2 subsets express FcepsiRI: correlation with serum IgE and allergic asthma. J Allergy Clin Immunol 2003;112:1132–1138.
  47. Novak N, Allam JP, Hagemann T, Jenneck C, Laffer S, Valenta R, Kochan J, Bieber T: Characterization of FcepsiRI-bearing CD123 blood dendritic cell antigen-2 plasmacytoid dendritic cells in atopic dermatitis. J Allergy Clin Immunol 2004;114:364–370.
  48. Mahnke K, Schmitt E, Bonifaz L, Enk AH, Jonuleit H: Immature, but not inactive: the tolerogenic function of immature dendritic cells. Immunol Cell Biol 2002;80:477–483.
  49. Farkas L, Kvale EO, Johansen FE, Jahnsen FL, Lund-Johansen F: Plasmacytoid dendritic cells activate allergen-specific TH2 memory cells: modulation by CpG oligodeoxynucleotides. J Allergy Clin Immunol 2004;114:436–443.
  50. Gilliet M, Liu YJ: Human plasmacytoid-derived dendritic cells and the induction of T-regulatory cells. Hum Immunol 2002;63:1149–1155.
  51. Jonuleit H, Schmitt E, Steinbrink K, Enk AH: Dendritic cells as a tool to induce anergic and regulatory T cells. Trends Immunol 2001;22:394–400.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: March 09, 2005
Accepted: July 07, 2005
Published online: November 18, 2005
Issue release date: December 2005

Number of Print Pages: 13
Number of Figures: 6
Number of Tables: 4

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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