Ophthalmic Research

Original Paper

Maintaining the Redox-Balance Intact: Gosha-Jinki-Gan but Not Insulin Activates Retinal Soluble Guanylate Cyclase in Diabetic Rats

Cameron-Schaefer S.a-c · Kondo K.b · Ishige A.a · Tsuyama S.d · Uchida K.e · Hanawa T.c · Suematsu M.b · Watanabe K.a

Author affiliations

Departments of aOriental Medicine and bBiochemistry and Integrative Medical Biology, Keio University School of Medicine, and cOriental Medicine Research Center of the Kitasato Institute, Tokyo, dDepartment of Veterinary Science, Osaka Prefecture University, Osaka, eLaboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya, Japan

Keywords: Diabetic retinopathyJapanese phytotherapy (Kampo)Gosha-jinki-ganPeroxynitriteLipid peroxideSoluble guanylate cyclase

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Ophthalmic Res 2006;38:95–104
https://doi.org/10.1159/000090451

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 09, 2005
Accepted: August 29, 2005
Published online: March 01, 2006
Issue release date: February 2006

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 0

ISSN: 0030-3747 (Print)
eISSN: 1423-0259 (Online)

For additional information: https://www.karger.com/ORE

Abstract

Strategies to prevent hyperglycemia-induced cytotoxic reactive oxygen species in the retina include the prevention of free radical production, activation of radical-scavenging capacities and inhibition of aldose reductase. This study examined the effect of the standardized Japanese herbal extract product gosha-jinki-gan (GJG) in comparison to insulin treatment in the rat retina. Diabetes was induced in male Wistar rats by single injection of streptozotocin (50 mg/kg i.p.). At 6 and 12 weeks, eyecups were removed for immunohistochemistry. At 12 weeks, lipid peroxidation (tested with the antiacrolein antibody, Ab5F6) was enhanced significantly in the untreated diabetic group. This effect was absent in both treatment groups, notably in the outer retina. A similar result was obtained for nitrotyrosine overproduction. As an early treatment effect, GJG – but not insulin – enhanced soluble guanylate cyclase (sGC) activation (using the function-sensing antibody, MoAb 3221). GJG not only reduces nitroxidative stress and lipid peroxidation in the retina, it also ameliorates glucose metabolism within the cells. We propose that the high glucose turnover in the insulin-treated model disturbs the intracellular redox equilibrium, one result of which might be the impaired sGC activation.

© 2006 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: June 09, 2005
Accepted: August 29, 2005
Published online: March 01, 2006
Issue release date: February 2006

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 0

ISSN: 0030-3747 (Print)
eISSN: 1423-0259 (Online)

For additional information: https://www.karger.com/ORE

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