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Original Paper

Validation of Ultrasonography to Evaluate Murine Orthotopic Oral Cavity Tumors

Pezold J.C.a · Zinn K.b · Talbert M.A.a · Desmond R.b · Rosenthal E.L.a

Author affiliations

aDepartment of Surgery, Division of Otolaryngology – Head and Neck Surgery, and bComprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, Ala., USA

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ORL 2006;68:159–163

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: January 28, 2005
Accepted: September 21, 2005
Published online: April 20, 2006
Issue release date: April 2006

Number of Print Pages: 5
Number of Figures: 5
Number of Tables: 0

ISSN: 0301-1569 (Print)
eISSN: 1423-0275 (Online)

For additional information: https://www.karger.com/ORL

Abstract

Background: The murine orthotopic oral cavity tumor model allows evaluation of tumor growth and invasion. Currently, serial measurements of tissue growth are difficult to obtain since invasive procedures or animal sacrifice is necessary to evaluate tumor size. High-resolution ultrasound was evaluated as a noninvasive method to monitor tumor size in vivo. Methods: Sixteen immunodeficient mice, age 9 weeks, were injected transcervically with a human squamous cell carcinoma cell line into the tongue, and tumor volume was assessed by high-frequency ultrasound at 11 days. The animals were subsequently sacrificed and the tumors processed for histology. Tumor size was then calculated by caliper measurement in two dimensions. Results: Tumor dimensions obtained using ultrasound were found to significantly correlate with the histologic measurements (Spearman coefficient 0.90, p < 0.0001). Tumor dimensions were on average larger using ultrasound versus histologic measurements, although this was not significantly different than zero (95% confidence interval –13.96 to 62.37 mm2). Conclusions: High-resolution ultrasound accurately measures tumor volume in the murine orthotopic oral cavity tumor model without sacrifice.

© 2006 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: January 28, 2005
Accepted: September 21, 2005
Published online: April 20, 2006
Issue release date: April 2006

Number of Print Pages: 5
Number of Figures: 5
Number of Tables: 0

ISSN: 0301-1569 (Print)
eISSN: 1423-0275 (Online)

For additional information: https://www.karger.com/ORL


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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