Hormone Research in Paediatrics

Original Paper

Effects of Phytoestrogen Extracts Isolated from Rye, Green and Yellow Pea Seeds on Hormone Production and Proliferation of Trophoblast Tumor Cells Jeg3

Matscheski A.a · Richter D.-U.c · Hartmann A.-M.a · Effmert U.a · Jeschke U.b · Kupka M.S.b · Abarzua S.a · Briese V.c · Ruth W.d · Kragl U.d · Piechulla B.a

Author affiliations

aDepartment of Biological Sciences, University of Rostock, Rostock, bFirst Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich, Departments of cObstetrics and Gynaecology and dTechnical Chemistry, University of Rostock, Rostock, Germany

Keywords: Jeg3RyeGreen and yellow peaEstradiolProgesterone5-Bromo-2′-deoxy-Uridine

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Horm Res 2006;65:276–288
https://doi.org/10.1159/000092591

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 13, 2005
Accepted: February 20, 2006
Published online: June 09, 2006
Issue release date: June 2006

Number of Print Pages: 13
Number of Figures: 11
Number of Tables: 0

ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)

For additional information: https://www.karger.com/HRP

Abstract

Background: Phytoestrogens are a diverse group of non-steroidal plant compounds. Because they have chemical structures similar to estrogens they are able to bind on estrogen receptors in humans. Objectives: In this study, we tested the effects of crude phytoestrogen extracts from rye (Secale cereale), green pea (Pisum sativum) and yellow pea seeds (Pisum sativum cv.) on cell proliferation and the production of progesterone in trophoblast tumor cells of the cell line Jeg3. Methods: Isoflavone extracts from green and yellow pea seeds and lignan extracts from rye seeds were obtained, using different extraction methods. Isolated extracts were incubated in different concentrations with trophoblast tumor cells. Untreated cells were used as controls. At designated times, aliquots were removed and tested for estradiol and progesterone production. In addition, we tested the effects of the phytoestrogen extracts on cell proliferation. Results: Cell proliferation is significantly inhibited by potential phytoestrogens isolated from rye, green and yellow pea seeds in trophoblast tumor cells of the cell line Jeg3. We found a correlation between the effects of proliferation and production of estradiol in isoflavone extracts from green and yellow pea seeds in Jeg3 cells. In addition, higher concentrations of isoflavones isolated from green pea seeds and lignans from rye showed also a inhibition of progesterone production whereas higher concentrations of rye lignans elevated estradiol production in Jeg3 cells. Conclusion: A useful indicator test system for potential phytoestrogens could be established. Based on the obtained results it is proposed that green and yellow pea seeds contain measurable concentrations of isoflavones and rye seeds contain lignans which can be isolated and used for special human diet programs.

© 2006 S. Karger AG, Basel



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References

  1. Setchell KD: Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. Am J Clin Nutr 1998;68:1333S–1346S.
  2. Price KR, Fenwick GR: Naturally occurring oestrogens in foods – a review. Food Addit Contam 1985;2:73–106.
    External Resources
  3. Manas ES, Xu ZB, Unwalla RJ, Somers WS: Understanding the selectivity of genistein for human estrogen receptor-beta using X-ray crystallography and computational methods. Structure (Camb) 2004;12:2197–2207.
    External Resources
  4. Messina MJ, Persky V, Setchell KD, Barnes S: Soy intake and cancer risk: a review of the in vitro and in vivo data. Nutr Cancer 1994;21:113–131.
    External Resources
  5. Park D, Huang T, Frishman WH: Phytoestrogens as cardioprotective agents. Cardiol Rev 2005;13:13–17.
    External Resources
  6. Begum AN, Nicolle C, Mila I, Lapierre C, Nagano K, Fukushima K, Heinonen SM, Adlercreutz H, Remesy C, Scalbert A: Dietary lignins are precursors of mammalian lignans in rats. J Nutr 2004;134:120–127.
    External Resources
  7. Adlercreutz H: Phytoestrogens: epidemiology and a possible role in cancer protection. Environ Health Perspect 1995;7(suppl):103–112.
    External Resources
  8. Fournier DB, Erdman JW Jr, Gordon GB: Soy, its components, and cancer prevention: a review of the in vitro, animal, and human data. Cancer Epidemiol Biomarkers Prev 1998;7:1055–1065.
    External Resources
  9. Wei H, Wei L, Frenkel K, Bowen R, Barnes S: Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein. Nutr Cancer 1993;20:1–12.
    External Resources
  10. Glazier MG, Bowman MA: A review of the evidence for the use of phytoestrogens as a replacement for traditional estrogen replacement therapy. Arch Intern Med 2001;161:1161–1172.
    External Resources
  11. Akiyama T, Ogawara H: Use and specificity of genistein as inhibitor of protein-tyrosine kinases. Methods Enzymol 1991;201:362–370.
    External Resources
  12. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, Fukami Y: Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 1987;262:5592–5595.
    External Resources
  13. Su SJ, Yeh TM, Chuang WJ, Ho CL, Chang KL, Cheng HL, Liu HS, Hsu PY, Chow NH: The novel targets for anti-angiogenesis of genistein on human cancer cells. Biochem Pharmacol 2005;69:307–318.
    External Resources
  14. Kaplanski O, Shemesh M, Berman A: Effects of phyto-oestrogens on progesterone synthesis by isolated bovine granulosa cells. J Endocrinol 1981;89:343–348.
    External Resources
  15. Whitehead SA, Cross JE, Burden C, Lacey M: Acute and chronic effects of genistein, tyrphostin and lavendustin A on steroid synthesis in luteinized human granulosa cells. Hum Reprod 2002;17:589–594.
    External Resources
  16. Basly JP, Lavier MC: Dietary phytoestrogens: potential selective estrogen enzyme modulators? Planta Med 2005;71:287–294.
    External Resources
  17. Chen S, Zhang F, Sherman MA, Kijima I, Cho M, Yuan YC, Toma Y, Osawa Y, Zhou D, Eng ET: Structure-function studies of aromatase and its inhibitors: a progress report. J Steroid Biochem Mol Biol 2003;86:231–237.
    External Resources
  18. Morrissey C, Watson RW: Phytoestrogens and prostate cancer. Curr Drug Targets 2003;4:231–241.
    External Resources
  19. Papiez M, Gancarczyk M, Bilinska B: The compounds from the hollyhock extract (Althaea rosea Cav. var. nigra) affect the aromatization in rat testicular cells in vivo and in vitro. Folia Histochem Cytobiol 2002;40:353–359.
    External Resources
  20. Almstrup K, Fernandez MF, Petersen JH, Olea N, Skakkebaek NE, Leffers H: Dual effects of phytoestrogens result in u-shaped dose-response curves. Environ Health Perspect 2002;110:743–748.
    External Resources
  21. Eng ET, Williams D, Mandava U, Kirma N, Tekmal RR, Chen S: Suppression of aromatase (estrogen synthetase) by red wine phytochemicals. Breast Cancer Res Treat 2001;67:133–146.
    External Resources
  22. Zhao J, Mak P, Tchoudakova A, Callard G, Chen S: Different catalytic properties and inhibitor responses of the goldfish brain and ovary aromatase isozymes. Gen Comp Endocrinol 2001;123:180–191.
    External Resources
  23. Kirk CJ, Harris RM, Wood DM, Waring RH, Hughes PJ: Do dietary phytoestrogens influence susceptibility to hormone-dependent cancer by disrupting the metabolism of endogenous oestrogens? Biochem Soc Trans 2001;29:209–216.
    External Resources
  24. Krazeisen A, Breitling R, Moller G, Adamski J: Phytoestrogens inhibit human 17beta-hydroxysteroid dehydrogenase type 5. Mol Cell Endocrinol 2001;171:151–162.
    External Resources
  25. Chen S: Aromatase and breast cancer. Front Biosci 1998;3:d922–d933.
  26. Kao YC, Zhou C, Sherman M, Laughton CA, Chen S: Molecular basis of the inhibition of human aromatase (estrogen synthetase) by flavone and isoflavone phytoestrogens: a site-directed mutagenesis study. Environ Health Perspect 1998;106:85–92.
    External Resources
  27. Adlercreutz H, Bannwart C, Wahala K, Makela T, Brunow G, Hase T, Arosemena PJ, Kellis JT Jr, Vickery LE: Inhibition of human aromatase by mammalian lignans and isoflavonoid phytoestrogens. J Steroid Biochem Mol Biol 1993;44:147–153.
    External Resources
  28. Plessow D, Waldschlager J, Richter DU, Jeschke U, Bruer G, Briese V, Friese K: Effects of phytoestrogens on the trophoblast tumour cell lines BeWo and Jeg3. Anticancer Res 2003;23:1081–1086.
    External Resources
  29. Waldschlager J, Bergemann C, Ruth W, Effmert U, Jeschke U, Richter DU, Kragl U, Piechulla B, Briese V: Flax-seed extracts with phytoestrogenic effects on a hormone receptor-positive tumour cell line. Anticancer Res 2005;25:1817–1822.
    External Resources
  30. Mylonas I, Jeschke U, Makovitzky J, Winkler L, Richter DU, Friese K, Briese V: Immunohistochemical expression of steroid receptors and glycodelin A in isolated proliferative human endometrial glandular cells after stimulation with tamoxifen and phytoestrogens (genistein and daidzein). Anticancer Res 2003;23:1119–1125.
    External Resources
  31. Franz G KH: Allgemeine Nachweismethoden für Flavonoide in Drogen; in Franz G KH (ed): Drogen und Naturstoffe. Grundlagen und Praxis der chemischen Analyse.Berlin, Heidelberg, Springer Verlag, 1992, p 129.
  32. Matscheski A: Untersuchungen zur Wirkung von Pflanzenextrakten aus Linum usitatissimum, Secale sereale und Pisum sativum auf die Zellproliferation und Progesteronproduktion von humanen Trophoblasttumorzellen; in Fachbereich Biowissenschaften, Biochemie. Rostock, Universität Rostock, 2003.
  33. Luyengi L SN, Fong HHS, Pezzuto JM, Kinghorn AD: A lignan and four terpenoids from Brucea javanica that induce differentiation with cultured HL-60 promeolytic leukemia cells. Phytochemistry 1996;43:409–412.
    External Resources
  34. Adlercreutz H, Mazur W: Phyto-oestrogens and Western diseases. Ann Med 1997;29:95–120.
    External Resources
  35. Anderson JJ, Garner SC: Phytoestrogens and bone. Baillieres Clin Endocrinol Metab 1998;12:543–557.
    External Resources
  36. An J, Tzagarakis-Foster C, Scharschmidt TC, Lomri N, Leitman DC: Estrogen receptor beta-selective transcriptional activity and recruitment of coregulators by phytoestrogens. J Biol Chem 2001;276:17808–17814.
    External Resources
  37. Kuiper GG, Lemmen JG, Carlsson B, Corton JC, Safe SH, van der Saag PT, van der Burg B, Gustafsson JA: Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology 1998;139:4252–4263.
    External Resources
  38. Barnes S: The chemopreventive properties of soy isoflavonoids in animal models of breast cancer. Breast Cancer Res Treat 1997;46:169–179.
    External Resources
  39. Lamartiniere CA, Cotroneo MS, Fritz WA, Wang J, Mentor-Marcel R, Elgavish A: Genistein chemoprevention: timing and mechanisms of action in murine mammary and prostate. J Nutr 2002;132:552S–558S.
  40. Lamartiniere CA, Zhang JX, Cotroneo MS: Genistein studies in rats: potential for breast cancer prevention and reproductive and developmental toxicity. Am J Clin Nutr 1998;68:1400S–1405S.
  41. Allred CD, Allred KF, Ju YH, Goeppinger TS, Doerge DR, Helferich WG: Soy processing influences growth of estrogen-dependent breast cancer tumors. Carcinogenesis 2004;25:1649–1657.
    External Resources
  42. Allred CD, Allred KF, Ju YH, Virant SM, Helferich WG: Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent (MCF-7) tumors in a dose-dependent manner. Cancer Res 2001;61:5045–5050.
    External Resources
  43. Sathyamoorthy N, Wang TT: Differential effects of dietary phyto-oestrogens daidzein and equol on human breast cancer MCF-7 cells. Eur J Cancer 1997;33:2384–2389.
    External Resources
  44. Zaizen Y, Higuchi Y, Matsuo N, Shirabe K, Tokuda H, Takeshita M: Antitumor effects of soybean hypocotyls and soybeans on the mammary tumor induction by N-methyl-n-nitrosourea in F344 rats. Anticancer Res 2000;20:1439–1444.
    External Resources
  45. Ho CK, Chi CW, Yu KJ, Wang SY: Tamoxifen-mediated anti-cellular effect against a choriocarcinoma cell line. Int J Oncol 1998;12:1171–1176.
    External Resources
  46. Jiang SW, Lloyd RV, Jin L, Eberhardt NL: Estrogen receptor expression and growth-promoting function in human choriocarcinoma cells. DNA Cell Biol 1997;16:969–977.
    External Resources

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 13, 2005
Accepted: February 20, 2006
Published online: June 09, 2006
Issue release date: June 2006

Number of Print Pages: 13
Number of Figures: 11
Number of Tables: 0

ISSN: 1663-2818 (Print)
eISSN: 1663-2826 (Online)

For additional information: https://www.karger.com/HRP

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2006, Vol.65, No. 6

June 2006

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A.M. and D.-U.R. contributed equally to this work.
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