Pharmacology

Original Paper

Delta-Sleep-Inducing Peptide Potentiates Anticonvulsive Activity of Valproate against Metaphit-Provoked Audiogenic Seizure in Rats

Hrnčić D.a · Stanojlović O.a · Živanović D.a · Šušić V.b

Author affiliations

aLaboratory of Neurophysiology, Department of Physiology, School of Medicine, University of Belgrade, and bDepartment of Medical Sciences, Serbian Academy of Science and Arts, Belgrade, Serbia and Montenegro

Keywords: Audiogenic seizuresMetaphitValproateDelta-sleep-inducing peptide

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Pharmacology 2006;77:78–84
https://doi.org/10.1159/000093001

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 15, 2006
Accepted: February 24, 2006
Published online: June 02, 2006
Issue release date: May 2006

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: https://www.karger.com/PHA

Abstract

The effect of delta-sleep-inducing peptide (DSIP) on the anticonvulsive activity of a nonprotective valproate (VPA) dose in a metaphit model of generalized, reflex audiogenic seizures in adult Wistar rats was studied. The animals that received metaphit (10 mg/kg) were exposed to audiogenic stimulation (100 ± 3 dB, 60 s) at hourly intervals. Metaphit-treated rats displaying seizures in 8 previous tests were i.p. injected with VPA (50 mg/kg) or DSIP (1.0 mg/kg) or their combination. Latency to seizure was behaviorally assessed. The EEGs and power spectra were recorded and analyzed. Neurotoxicity was evaluated by the chimney test. DSIP or VPA alone expressed no significant effect on the latency duration, but their combination significantly prolonged latency to seizure during 6 h after injection, while inducing no significant motor impairment. Neither the applied drugs nor their combination abolished metaphit-provoked EEG epileptiform activity. The results show that DSIP potentiated anticonvulsive effects of a nonprotective VPA dose in a metaphit model of audiogenic seizures without influencing its neurotoxicity.

© 2006 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 15, 2006
Accepted: February 24, 2006
Published online: June 02, 2006
Issue release date: May 2006

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: https://www.karger.com/PHA

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2006, Vol.77, No. 2

May 2006

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