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Special Applications

Towards many colors in FISH on 3D-preserved interphase nuclei

Walter J.b · Joffe B.a · Bolzer A.a · Albiez H.a · Benedetti P.A.d · Müller S.a · Speicher M.R.c · Cremer T.a · Cremer M.a · Solovei I.a

Author affiliations

aTILL I.D. GmbH, c\o BioImaging Zentrum, Martinsried; bChair of Anthropology and Human Genetics, Department of Biology II, Ludwig-Maximilians-University, Martinsried (Germany); cIPCF-CNR, Pisa (Italy); dTechnical University and GSF, Institute of Human Genetics, Munich (Germany)

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Cytogenet Genome Res 114:367–378 (2006)

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Article / Publication Details

First-Page Preview
Abstract of Special Applications

Published online: September 01, 2006
Issue release date: August 2006

Number of Print Pages: 12
Number of Figures: 4
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome – one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested.

© 2006 S. Karger AG, Basel


References

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Article / Publication Details

First-Page Preview
Abstract of Special Applications

Published online: September 01, 2006
Issue release date: August 2006

Number of Print Pages: 12
Number of Figures: 4
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR


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