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Original Paper

The Potential Allergenicity of Two 2S Albumins from Soybean (Glycine max): A Protein Microarray Approach

Lin J.a · Shewry P.R.b · Archer D.B.c · Beyer K.e · Niggemann B.e · Haas H.f · Wilson P.d · Alcocer M.J.C.a

Author affiliations

aSchool of Biosciences, Division of Nutritional Sciences, University of Nottingham, Loughborough, bRothamsted Research, Harpenden, cSchool of Biology, University of Nottingham, Nottingham, and dRegional Immunology Department, Saint Mary’s Hospital, Manchester, UK; eKlinik für Pädiatrie m.S. Pneumologie und Immunologie, Charité-Universitätsmedizin Berlin, Berlin, and fZelluläre Allergologie, Forschungszentrum Borstel, Borstel, Germany

Related Articles for ""

Int Arch Allergy Immunol 2006;141:91–102

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 19, 2005
Accepted: March 28, 2006
Published online: September 20, 2006
Issue release date: September 2006

Number of Print Pages: 12
Number of Figures: 3
Number of Tables: 3

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA

Abstract

Background: The 2S albumins are a group of storage proteins that occur widely in seeds of dicotyledonous plants. The widespread distribution and stability to digestion of allergenic 2S albumins raise the question of why some members of this family present in important food sources, such as soybean, are not regarded as major allergens. Methods: The pepsinolytic stability of two 2S albumins from soybean seed was determined using simulated gastric fluid. Using a new protein microarray system, IgE binding to these soybean 2S albumins was studied with the sera from 23 European individuals allergic to soybean. In order to validate the microarray result, two of the sera were selected and further tested using the micro-ELISA and UniCAP system. Results: Both albumins exhibited high stability to digestion similar to other allergenic members of the 2S albumin, trypsin/amylase inhibitor and lipid transfer protein superfamily. None of the patients was found to have IgE specific to soybean 2S albumins by the microarray system, and this result was in agreement with the results from the micro-ELISA and UniCAP system.Conclusions: The results from microarray, micro-ELISA and UniCAP system suggested that the 2S albumins from soybean are not major allergens within the patient population analyzed.

© 2006 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 19, 2005
Accepted: March 28, 2006
Published online: September 20, 2006
Issue release date: September 2006

Number of Print Pages: 12
Number of Figures: 3
Number of Tables: 3

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: https://www.karger.com/IAA


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